Efficient inhibition of hepatitis B virus replication by small interfering RNAs targeted to the viral X gene in mice

Virus Research

Volumn 119, Issue 2, 2006, Pages 146-153

  Shin, Duckhyang ^a   Kim, Soo In ^a   Kim, Meehyein ^a   Park, Mahnhoon ^a  

^a Mogam Biotechnology Research Institute   (South Korea)

Author keywords

Chronic infection; HBV; Hydrodynamic injection; RNAi; siRNA

Indexed keywords

HEPATITIS B VIRUS X PROTEIN; MESSENGER RNA; SMALL INTERFERING RNA;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTIVIRAL ACTIVITY; ARTICLE; CELL CULTURE; CONTROLLED STUDY; DRUG TARGETING; FEMALE; GENETIC TRANSFECTION; HEPATITIS B; HEPATITIS B VIRUS; HUMAN; HUMAN CELL; HYDRODYNAMICS; MOUSE; NONHUMAN; PRIORITY JOURNAL; VIRUS GENOME; VIRUS INHIBITION; VIRUS REPLICATION;

ANIMALS; BLOTTING, NORTHERN; CELL LINE; DISEASE MODELS, ANIMAL; ENZYME-LINKED IMMUNOSORBENT ASSAY; FEMALE; GENE THERAPY; HEPATITIS B; HEPATITIS B SURFACE ANTIGENS; HEPATITIS B VIRUS; HUMANS; MICE; MICE, INBRED C57BL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA, VIRAL; TRANS-ACTIVATORS; VIRUS REPLICATION;

ANIMALIA; HEPATITIS B VIRUS;

EID: 33646816447     PISSN: 01681702     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.virusres.2005.12.012     Document Type: Article

References (35)

1. Bergametti F., Prigent S., Luber B., Benoit A., Tiollais P., Sarasin A., and Transy C. The proapoptotic effect of hepatitis B virus HBx protein correlates with its transactivation activity in stably transfected cell lines. Oncogene 18 (1999) 2860-2871
2. Bosher J.M., and Labouesse M. RNA interference: genetic wand and genetic watchdog. Nat. Cell Biol. 2 (2000) E31-E36
3. Cromlish J.A. Hepatitis B virus-induced hepatocellular carcinoma: possible roles for HBx. Trends Microbiol. 4 (1996) 270-274
4. Dienstag J.L., Cianciara J., Karayalcin S., Kowdley K.V., Willems B., Plisek S., Woessner M., Gardner S., and Schiff E. Durability of serologic response after lamivudine treatment of chronic hepatitis B. Hepatology 37 (2003) 748-755
5. Elbashir S.M., Harborth J., Lendeckel W., Yalcin A., Weber K., and Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 411 (2001) 494-498
6. Elbashir S.M., Lendeckel W., and Tuschl T. RNA interference is mediated by 21- and 22-nucleotide RNAs. Genes Dev. 15 (2001) 188-200
7. Fujiyama A., Miyanohara A., Nozaki C., Yoneyama T., Ohtomo N., and Matsubara K. Cloning and structural analyses of hepatitis B virus DNAs, subtype adr. Nucleic Acids Res. 11 (1983) 4601-4610
8. Ganem D., and Varmus H.E. The molecular biology of the hepatitis B viruses. Annu. Rev. Biochem. 56 (1987) 651-693
9. Giladi H., Ketzinel-Gilad M., Rivkin L., Felig Y., Nussbaum O., and Galun E. Small interfering RNA inhibits hepatitis B virus replication in mice. Mol. Ther. 8 (2003) 769-776
10. Gitlin L., Karelsky S., and Andino R. Short interfering RNA confers intracellular antiviral immunity in human cells. Nature 418 (2002) 430-434
11. Guidotti L.G., Matzke B., Schaller H., and Chisari F.V. High-level hepatitis B virus replication in transgenic mice. J. Virol. 69 (1995) 6158-6169
12. Hammond S.M., Bernstein E., Beach D., and Hannon G.J. An RNA-directed nuclease mediates post-transcriptional gene silencing in Drosophila cells. Nature 404 (2000) 293-296
13. Hannon G.J. RNA interference. Nature 418 (2002) 244-251
14. Jaboli M.F., Fabbri C., Liva S., Azzaroli F., Nigro G., Giovanelli S., Ferrara F., Miracolo A., Marchetto S., Montagnani M., Colecchia A., Festi D., Reggiani L.B., Roda E., and Mazzella G. Long-term alpha interferon and lamivudine combination therapy in non-responder patients with anti-HBe-positive chronic hepatitis B: results of an open, controlled trial. World J. Gastroenterol. 9 (2003) 1491-1495
15. Kekule A.S., Lauer U., Weiss L., Luber B., and Hofschneider P.H. Hepatitis B virus transactivator HBx uses a tumour promoter signalling pathway. Nature 361 (1993) 742-745
16. Kim D.H., Behlke M.A., Rose S.D., Chang M.S., Choi S., and Rossi J.J. Synthetic dsRNA Dicer substrates enhance RNAi potency and efficacy. Nat. Biotechnol. 23 (2005) 222-226
17. Klein C., Bock C.T., Wedemeyer H., Wustefeld T., Locarnini S., Dienes H.P., Kubicka S., Manns M.P., and Trautwein C. Inhibition of hepatitis B virus replication in vivo by nucleoside analogues and siRNA. Gastroenterology 125 (2003) 9-18
18. Lee W.M. Hepatitis B virus infection. N. Engl. J. Med. 337 (1997) 1733-1745
19. Lin O.S., and Keeffe E.B. Current treatment strategies for chronic hepatitis B and C. Annu. Rev. Med. 52 (2001) 29-49
20. Liu F., Song Y., and Liu D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther. 6 (1999) 1258-1266
21. McCaffrey A.P., Nakai H., Pandey K., Huang Z., Salazar F.H., Xu H., Wieland S.F., Marion P.L., and Kay M.A. Inhibition of hepatitis B virus in mice by RNA interference. Nat. Biotechnol. 21 (2003) 639-644
22. Morrissey D.V., Lockridge J.A., Shaw L., Blanchard K., Jensen K., Breen W., Hartsough K., Machemer L., Radka S., Jadhav V., Vaish N., Zinnen S., Vargeese C., Bowman K., Shaffer C.S., Jeffs L.B., Judge A., MacLachlan I., and Polisky B. Potent and persistent in vivo anti-HBV activity of chemically modified siRNAs. Nat. Biotechnol. 23 (2005) 1002-1007
23. Nakatake H., Chisaka O., Yamamoto S., Matsubara K., and Koshy R. Effect of X protein on transactivation of hepatitis B virus promoters and on viral replication. Virology 195 (1993) 305-314
24. Novina C.D., Murray M.F., Dykxhoorn D.M., Beresford P.J., Riess J., Lee S.K., Collman R.G., Lieberman J., Shankar P., and Sharp P.A. siRNA-directed inhibition of HIV-1 infection. Nat. Med. 8 (2002) 681-686
25. Nykanen A., Haley B., and Zamore P.D. ATP requirements and small interfering RNA structure in the RNA interference pathway. Cell 107 (2001) 309-321
26. Paterlini P., Poussin K., Kew M., Franco D., and Brechot C. Selective accumulation of the X transcript of hepatitis B virus in patients negative for hepatitis B surface antigen with hepatocellular carcinoma. Hepatology 21 (1995) 313-321
27. Reynolds A., Leake D., Boese Q., Scaringe S., Marshall W.S., and Khvorova A. Rational siRNA design for RNA interference. Nat. Biotechnol. 22 (2004) 326-330
28. Soutschek J., Akinc A., Bramlage B., Charisse K., Constien R., Donoghue M., Elbashir S., Geick A., Hadwiger P., Harborth J., John M., Kesavan V., Lavine G., Pandey R.K., Racie T., Rajeev K.G., Rohl I., Toudjarska I., Wang G., Wuschko S., Bumcrot D., Koteliansky V., Limmer S., Manoharan M., and Vornlocher H.P. Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs. Nature 432 (2004) 173-178
29. Spandau D.F., and Lee C.H. Trans-activation of viral enhancers by the hepatitis B virus X protein. J. Virol. 62 (1988) 427-434
30. Uprichard S.L., Boyd B., Althage A., and Chisari F.V. Clearance of hepatitis B virus from the liver of transgenic mice by short hairpin RNAs. Proc. Natl. Acad. Sci. U.S.A. 102 (2005) 773-778
31. Wang W.L., London W.T., and Feitelson M.A. Hepatitis B x antigen in hepatitis B virus carrier patients with liver cancer. Cancer Res. 51 (1991) 4971-4977
32. Wilson J.A., Jayasena S., Khvorova A., Sabatinos S., Rodrigue-Gervais I.G., Arya S., Sarangi F., Harris-Brandts M., Beaulieu S., and Richardson C.D. RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells. Proc. Natl. Acad. Sci. U.S.A. 100 (2003) 2783-2788
33. Wu H.L., Huang L.R., Huang C.C., Lai H.L., Liu C.J., Huang Y.T., Hsu Y.W., Lu C.Y., Chen D.S., and Chen P.J. RNA interference-mediated control of hepatitis B virus and emergence of resistant mutant. Gastroenterology 128 (2005) 708-716
34. Xu Z., Yen T.S., Wu L., Madden C.R., Tan W., Slagle B.L., and Ou J.H. Enhancement of hepatitis B virus replication by its X protein in transgenic mice. J. Virol. 76 (2002) 2579-2584
35. Zheng L., Liu J., Batalov S., Zhou D., Orth A., Ding S., and Schultz P.G. An approach to genomewide screens of expressed small interfering RNAs in mammalian cells. Proc. Natl. Acad. Sci. U.S.A. 101 (2004) 135-140