Transfected HepG2 cells for evaluation of catechin effects on alcohol-induced CYP2E1 cytotoxicity

Journal of Microbiology and Biotechnology

Volumn 15, Issue 6, 2005, Pages 1310-1316

  Lee, Yoo Hyun ^a   Ho, Jin Nyoung ^a   Dong, Mi Sook ^a   Park, Chang Hwan ^b   Kim, Hye Kyung ^c   Hong, Bumshik ^a   Shin, Dong Hoon ^a   Cho, Hong Yon ^a  

^a Korea University   (South Korea)

^b Hanyang University   (South Korea)

^c Hanseo University   (South Korea)

Author keywords

Catechins; Cytochrome P450 2E1; Ethanol; HepG2 cells

Indexed keywords

3 (4,5 DIMETHYL 2 THIAZOLYL) 2,5 DIPHENYLTETRAZOLIUM BROMIDE; ALCOHOL; ANTIOXIDANT; CATECHIN; CATECHIN GALLATE; CYTOCHROME P450 2E1; EPICATECHIN; EPICATECHIN GALLATE; EPIGALLOCATECHIN; EPIGALLOCATECHIN GALLATE; GALLOCATECHIN; GREEN TEA EXTRACT; LACTATE DEHYDROGENASE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANTIOXIDANT ACTIVITY; ARTICLE; CELL DEATH; CELL PROTECTION; CELL STRAIN HEPG2; CELL STRUCTURE; CONCENTRATION RESPONSE; CYTOTOXICITY; GENETIC TRANSDUCTION; HUMAN; HUMAN CELL; OXIDATIVE STRESS; PROTEIN EXPRESSION;

EID: 33646736133     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (37)

1. Aguilar, C. N., M. Cruz, R. Rodriguez, G. Gutierrenz-Sanchez, A. Ramirez-Coronel, and C. Augur. 2004. Catechin degradation by several fungal strains isolated from Mexican desert. J. Microbiol. Biotechnol. 14: 426-429.
2. Bailey, S. M. and C. C. Cunningham. 1999. Acute and chronic ethanol increases reactive oxygen species generation and decreases viability in fresh, isolated rat hepatocytes. Hepatology 28: 1318-1326.
3. Balentine, D. A. and I. Paetau-Robinson. 1998. Tea as a source of dietary antioxidants with a potential role in prevention of chronic diseases, pp. 265-287. In G. Mazza and B. D. Oomah (eds.), Herbs, Botanicals, and Teas. CRC Press LLC, Florida.
4. Cameron, R. G., M. G. Neuman, N. H. Shear, G. Katz, S. Bellentani, and C. Tiribelli. 1998. Modulation of liver-specific cellular response to ethanol in vitro in HepG2 cells. Toxicol. In Vitro 12: 111-122.
5. Caro, A. A. and A. I. Cederbaum. 2004. Oxidative stress, toxicology, and pharmacology of CYP2E1. Annu. Rev. Pharmacol. Toxicol. 44: 27-42.
6. Castilla, R., R. González, D. Fouad, E. Fraga, and J. Muntané. 2004. Dual effect of ethanol on cell death in primary culture of human and rat hepatocytes. Alcohol Alcohol. 39: 290-296.
7. Cederbaum, A. I. 2003. Iron and CYP2E1-dependent oxidative stress and toxicity. Alcohol 30: 115-120.
8. Chen, L., X. Yang, H. Jiao, and B. Zhao. 2002. Tea catechins protect against lead-induced cytotoxicity, lipid peroxidation, and membrane fluidity in HepG2 cells. Toxicol. Sci. 69: 249-256.
9. Chen, Q. and A. I. Cederbaum. 1998. Cytotoxicity and apoptosis produced by cytochrome P450 2E1 in HepG2 cells. Mol. Pharmacol. 53: 638-648.
10. Dai, Y., J. Rashba-Step, and A. I. Cederbaum. 1993. Stable expression of human P4502E1 in HepG2 cells: Characterization of catalytic activities and production of reactive oxygen intermediates. Biochemistry 32: 6928-6937.
11. Donohue, T. M., D. L. Clemens, Jr., A. Galli, D. Crabb, N. Nieto, J. Kato, and S. S. Barve. 2001. Use of cultured cells in assessing ethanol toxicity and ethanol-related metabolism. Alcohol Clin. Exp. Res. 25: 87S-93S.
12. Eysseric, H., B. Gonthier, A. Soubeyran, M. J. Richard, D. Daveloose, and L. Barret. 2000. Effects of chronic ethanol exposure on acetaldehyde and free radical production by astrocytes in culture. Alcohol 21: 117-125.
13. Ikeda, I., M. Kobayashi, T. Hamada, K. Tsuda, H. Goto, K. Imaizumi, A. Nozawa, A. Sugimoto, and T. Kakuda. 2003. Heat-epimerized tea catechins rich in gallocatechin gallate and catechins gallate are more effective to inhibit cholesterol absorption than tea catechins rich in epigallocatechin gallate and epicatechin gallate. J. Agric. Food Chem. 51: 7303-7307.
14. Jamenez-Lopez, J. M. and A. I. Cederbaum. 2003. Green tea polyphenol epigallocatechin-3-gallate protects HepG2 cells against CYP2E1-dependent toxicity. Free Radic. Biol. Med. 36: 359-370.
15. Kagaya, N., Y. Tagawa, H. Nagashima, R. Saijo, M. Kawase, and K. Yaki. 2002. Suppression of cytotoxin-induced cell death in isolated hepatocytes by tea catechins. Eur. J. Pharmacol. 450: 231-236.
16. Kim, J.-H., H.-J. Kim, J.-H. Son, H.-N. Chun, J.-O. Yang, S.-J. Choi, N.-S. Paek, G.-H. Choi, and S.-K. Kim. 2003. Effect of Lactobacillus fermentum MG 590 on alcohol metabolism and liver function in rats. J. Microbiol. Biotechnol. 13: 919-925.
17. Kim, S., J. S. Kang, D. D. Jang, K. Lee, S. A. Kim, B. S. Han, and Y. I. Park. 2004. Differential gene expression in estradiol-3-benzoate-treated liver and chemically-induced hepatocellular carcinoma. J. Microbiol. Biotechnol. 14: 1286-1294.
18. Kurose, I., H. Higuchi, S. Miura, H. Saito, N. Watanabe, R. Hokari, M. Hirokawa, M. Takaishi, S. Zeki, T. Nakamura, H. Ebinuma, S. Kato, and H. Ishii. 1997. Oxidative stress-mediated apoptosis of hepatocytes exposed to acute ethanol intoxication. Hepatology 25: 368-378.
19. Lamarche, F., B. Gonthier, N. Signorini, H. Eysseric, and L. Baret. 2003. Acute exposure of cultured neurons to ethanol results in reversible DNA single-stranded breaks, whereas chronic exposure causes loss of cell viability. Alcohol Alcohol. 38: 550-558.
20. Lee, S.-K., C.-Y. Choi, J.-S. Ahn, J.-Y. Cho, C.-S. Park, and Y. J. Yoon. 2004. Identification of a cytochrome P450 hydroxylation gene involved in rifamycin biosynthesis by Amycolatopsis mediterranei S699. J. Microbiol. Biotechnol. 14: 356-362.
21. Lee, H. J. and M. B. Gu. 2003. Effect of benzo[a]pyrene on genes related to the cell cycle and cytochrome P450 of Saccharomyces cerevisiae. J. Microbiol. Biotechnol. 13: 624-627.
22. Li, Y., D. W. Walker, and M. A. King. 2001. Peroxide mediates ethanol-induced cytotoxicity in PC12 cells. Free Radic. Biol. Med. 30: 389-392.
23. Lieber, C. S. 2000. Alcohol: Its metabolism and interaction with nutrients. Annu. Rev. Nutr. 20: 395-430.
24. Mapoles, J., F. Berthou, A. Alexander, F. Simon, and J. F. Menez. 1993. Mammalian PC-12 cell genetically engineered for human cytochrome P450 2E1 expression. Eur. J. Biochem. 214: 735-745.
25. Muto, S., K. Fujita, Y. Yamazak, and T. Kamatak. 2001. Inhibition by green tea catechins of metabolic activation of procarcinogens by human cytochrome P450. Mutat. Res. 479: 197-206.
26. Nakagawa, T., M. Sawada, F. J. Gonzalez, T. Yokoi, and T. Kamaraki. 1996. Stable expression of human CYP2E1 in Chinese hamster cells: High sensitivity to N,N-dimethylnitrosamine in cytotoxicity testing. Mutat. Res. 360: 181-186.
27. Neuman, M. G., G. Koren, and C. Tribelli. 1993. In vitro assessment of the ethanol-induced hepatotoxicity in HepG2 cell line. Biochem. Biophys. Res. Commun. 197: 932-941.
28. Nouso, K., S. S. Thorgeirsson, and N. Battula. 1992. Stable expression of human cytochrome P450IIE1 in mammalian cells: Metabolic activation of nitrosodimethylamine and formation of adducts with cellular DNA. Cancer Res. 52: 1796-1800.
29. Ory, D. S., B. A. Neugeboren, and R. C. Mulligan. 1996. A stable human-derived packaging cell line for production of high titer retrovirus/vesicular stomatitis virus G pseudotypes. Proc. Natl. Acad. Sci. USA 93: 11400-11406.
30. Ostrowska, J., W. Łuczaj, I. Kasacka, A. Rózański, and E. Skrzydlewska. 2004. Green tea protects against ethanol-induced lipid peroxidation in rat organs. Alcohol 32: 25-32.
31. Sun, A. Y., M. Ingelman-Sundberg, E. Neve, H. Matsumoto, Y. Nishitani, Y. Minowa, Y. Fukui, S. M. Bailey, V. B. Patel, C. C. Cunningham, T. Zima, L. Fialova, L. Mikulikova, P. Popov, I. Malbohan, M. Janebova, K. Nespor, and G. Y. Sun. 2001. Ethanol and oxidative stress. Alcohol Clin. Exp. Res. 25: 237S-243S.
32. Waxman, D. J., D. P. Lapenson, T. Aoyama, H. V. Gelboin, F. J. Gonzalez, and K. Korzekwa. 1991. Steroid hormone hydroxylase specificities of eleven cDNA-expressed human cytochrome P450s. Arch. Biochem. Biophys. 290: 160-166.
33. Wu, D. and A. I. Cederbaum. 1996. Ethanol cytotoxicity to a transfected HepG2 cell line expressing human cytochrome P4502E1. J. Biol. Chem. 271: 23914-23919.
34. Wu, D. and A. I. Cederbaum. 1999. Ethanol-induced apoptosis to stable HepG2 cell lines expressing human cytochrome P-4502E1. Alcohol Clin. Exp. Res. 23: 67-76.
35. Xu, Y., M. A. Leo, and C. S. Lieber. 2003. Lycopene attenuates arachidonic acid toxicity in HepG2 cells overexpressing CYP2E1. Biochem. Biophys. Res. Commun. 303: 745-750.
36. Xu, Y., M. A. Leo, and C. S. Lieber. 2003. Lycopene attenuates alcoholic apoptosis in HepG2 cells expressing CYP2E1. Biochem. Biophys. Res. Commun. 308: 614-618.
37. Zhuge, J., Y. Luo, and Y. N. Yu. 2003. Heterologous expression of human cytochrome P450 2E1 in HepG2 cell line. World J. Gastroentrol. 9: 2732-2736.