High-level expression and purification of human thymidine kinase 1: Quaternary structure, stability, and kinetics

Protein Expression and Purification

Volumn 47, Issue 2, 2006, Pages 506-515

  Birringer, Markus S ^a   Perozzo, Remo ^b   Kut, Elvan ^a   Stillhart, Corinne ^a   Surber, Wanda ^a   Scapozza, Leonardo ^b   Folkers, Gerd ^c  

^a ETH ZURICH   (Switzerland)

^b EPGL   (Switzerland)

^c UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

CD spectroscopy; Expression; Human thymidine kinase 1; Kinetics; Purification; Quaternary structure

Indexed keywords

DNAK PROTEIN, E COLI; ESCHERICHIA COLI PROTEIN; HEAT SHOCK PROTEIN 70; RECOMBINANT PROTEIN; THYMIDINE; THYMIDINE KINASE; THYMIDINE KINASE 1;

AMINO ACID SEQUENCE; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE DELETION; GENETICS; HEAT; HUMAN; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; PROTEIN QUATERNARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HEAT; HSP70 HEAT-SHOCK PROTEINS; HUMANS; KINETICS; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS; SEQUENCE DELETION; SUBSTRATE SPECIFICITY; THYMIDINE; THYMIDINE KINASE;

EID: 33646586729     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2006.01.001     Document Type: Article

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