Application of reverse transcriptase PCR-based T-RFLP to perform semi-quantitative analysis of metabolically active bacteria in dairy fermentations

Journal of Microbiological Methods

Volumn 65, Issue 2, 2006, Pages 268-277

  Sánchez, Jorge I ^a   Rossetti, Lia ^b   Martínez, Beatriz ^a   Rodríguez, Ana ^a   Giraffa, Giorgio ^b  

^a DEPARTMENT OF MICROBIOLOGY AND BIOCHEMISTRY OF DAIRY PRODUCTS   (Spain)

^b ISTITUTO NAZIONALE DELLA NUTRIZIONE   (Italy)

Author keywords

Dairy starters; Reverse transcriptase PCR; Semi quantification; T RFLP

Indexed keywords

BACTERIAL RNA; PRIMER RNA; RIBOSOME RNA; RNA 16S; SINGLE STRANDED RNA;

AMPLICON; ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; CHIMERA; CONTROLLED STUDY; DAIRY INDUSTRY; DNA DETERMINATION; FERMENTATION; LACTIC ACID BACTERIUM; LACTOCOCCUS LACTIS; LEUCONOSTOC; LEUCONOSTOC CITREUM; MICROBIAL ACTIVITY; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ANIMALS; CHEESE; CULTURE MEDIA; DAIRYING; DNA PRIMERS; DNA, BACTERIAL; FERMENTATION; LACTOCOCCUS LACTIS; LEUCONOSTOC; MILK; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S;

BACTERIA (MICROORGANISMS); LACTOCOCCUS LACTIS; LEUCONOSTOC; LEUCONOSTOC CITREUM; LEUCONOSTOC LACTIS;

EID: 33645969304     PISSN: 01677012     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.mimet.2005.07.018     Document Type: Article

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