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M.A. Carmell, Z. Xuan, M.Q. Zhang, and G.J. Hannon The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis Genes Dev 16 2002 2733 2742
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M.A. Carmell, and G.J. Hannon RNase III enzymes and the initiation of gene silencing Nat Struct Mol Biol 11 2004 214 218
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A. Lingel, B. Simon, E. Izaurralde, and M. Sattler Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain Nature 426 2003 465 469
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The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes
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J.J. Song, J. Liu, N.H. Tolia, J. Schneiderman, S.K. Smith, R.A. Martienssen, G.J. Hannon, and L. Joshua-Tor The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes Nat Struct Biol 10 2003 1026 1032
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K.S. Yan, S. Yan, A. Farooq, A. Han, L. Zeng, and M.M. Zhou Structure and conserved RNA binding of the PAZ domain Nature 426 2003 468 474
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A. Lingel, B. Simon, E. Izaurralde, and M. Sattler Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain Nat Struct Mol Biol 11 2004 576 577 This study describes the solution structure of a PAZ domain in complex with single-stranded oligonucleotides.
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J.B. Ma, K. Ye, and D.J. Patel Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain Nature 429 2004 318 322 The crystal structure of hAgo1 PAZ domain in complex with a 'mini-siRNA' showing the details of the interactions between the PAZ domain and the RNA, in particular the interactions with the 3'-overhang.
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J.J. Song, S.K. Smith, G.J. Hannon, and L. Joshua-Tor Crystal structure of Argonaute and its implications for RISC slicer activity Science 305 2004 1434 1437 The first crystal structure of a full-length Argonaute reveals its function as 'Slicer' - the catalytic component of RISC.
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Song, J.J.1
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J. Liu, M.A. Carmell, F.V. Rivas, C.G. Marsden, J.M. Thomson, J.J. Song, S.M. Hammond, L. Joshua-Tor, and G.J. Hannon Argonaute2 is the catalytic engine of mammalian RNAi Science 305 2004 1437 1441 This study shows that of the four human Argonautes, only Argonaute2 functions as Slicer.
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Liu, J.1
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Hannon, G.J.9
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Purified Argonaute2 and an siRNA form recombinant human RISC
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F.V. Rivas, N.H. Tolia, J.J. Song, J.P. Aragon, J. Liu, G.J. Hannon, and L. Joshua-Tor Purified Argonaute2 and an siRNA form recombinant human RISC Nat Struct Mol Biol 12 2005 340 349 The presence of human Argonaute2 with an siRNA is sufficient for slicer activity. This system is used to show which features of RISC activities reside in Argonaute and which must reside in a different component of RISC. The active site of Argonaute is shown here to have a 'DDH' motif.
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Nat Struct Mol Biol
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M. Nowotny, S.A. Gaidamakov, R.J. Crouch, and W. Yang Crystal structures of RNase H bound to an RNA/DNA hybrid: substrate specificity and metal-dependent catalysis Cell 121 2005 1005 1016 The first RNase H structure in complex with its substrate provides important insights that are relevant for Argonaute substrate recognition and slicing activity.
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Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity
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J.S. Parker, S.M. Roe, and D. Barford Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity EMBO J 23 2004 4727 4737
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Y.R. Yuan, Y. Pei, J.B. Ma, V. Kuryavyi, M. Zhadina, G. Meister, H.Y. Chen, Z. Dauter, T. Tuschl, and D.J. Patel Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage Mol Cell 19 2005 405 419 Crystal structure of another archaeal full-length Argonaute protein showing domain movement of the PAZ domain. It also shows it to have preference for DNA-RNA hybrid substrates.
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Mol Cell
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Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs
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N. Baumberger, and D.C. Baulcombe Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs Proc Natl Acad Sci USA 102 2005 11928 11933
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J.K. Kim, H.W. Gabel, R.S. Kamath, M. Tewari, A. Pasquinelli, J.F. Rual, S. Kennedy, M. Dybbs, N. Bertin, and J.M. Kaplan Functional genomic analysis of RNA interference in C. elegans Science 308 2005 1164 1167
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