Functional genomic analysis of RNA interference in C. elegans

Science

Volumn 308, Issue 5725, 2005, Pages 1164-1167

  Kim, John K ^a,b   Gabel, Harrison W ^a,b   Kamath, Ravi S ^a,b,d   Tewari, Muneesh ^b,c   Pasquinelli, Amy ^a,b,e   Rual, Jean François ^b,c   Kennedy, Scott ^a,b,f   Dybbs, Michael ^a,b   Bertin, Nicolas ^b,c   Kaplan, Joshua M ^a,b   Vidal, Marc ^b,c   Ruvkun, Gary ^a,b  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c DANA FARBER CANCER INSTITUTE   (United States)

^d BRIGHAM AND WOMEN S HOSPITAL   (United States)

^e UNIVERSITY OF CALIFORNIA   (United States)

^f UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; ENZYMES; GENES; SENSORS;

DNA RECOMBINATION; DOUBLE-STRANDED RNA (DSRNA); RNA INTERFERENCE (RNAI); RNAI SENSOR STRAIN;

RNA;

DEOXYRIBONUCLEOPROTEIN; DOUBLE STRANDED RNA; HELICASE; NUCLEAR FACTOR; NUCLEASE; PROTEIN; PROTEIN PAZ; PROTEIN PIWI; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

GENETICS;

ARTICLE; CAENORHABDITIS ELEGANS; CHROMATIN; FUNCTIONAL GENOMICS; GENE CONTROL; GENE IDENTIFICATION; GENE SILENCING; GENE TARGETING; GENETIC ANALYSIS; GENETIC ENGINEERING; GENETIC SCREENING; NONHUMAN; NUCLEAR IMPORT; PRIORITY JOURNAL; RNA BINDING; RNA INTERFERENCE; TRANSGENE;

AMINO ACID MOTIFS; ANIMALS; CAENORHABDITIS ELEGANS; CAENORHABDITIS ELEGANS PROTEINS; GENE LIBRARY; GENE SILENCING; GENES, HELMINTH; GENOME; GENOMICS; PROTEIN INTERACTION MAPPING; PROTEIN STRUCTURE, TERTIARY; RNA INTERFERENCE; RNA PRECURSORS; RNA, DOUBLE-STRANDED; RNA, HELMINTH; TRANSGENES;

CAENORHABDITIS ELEGANS;

EID: 21044446786     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1109267     Document Type: Article

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29. We thank C. C. Mello for sharing unpublished data regarding rde-5. We also thank J. S. Ahn, D. E. Hill, and T. Hirozane-Kishikawa for technical assistance; H. Y. Mak for the ttx-3::RFP plasmid; W. Kelly for providing the PD7271 strain; A. Frand for extensive comments on the manuscript; and P. Hu, B. Reinhardt, S. Fischer, D. Kim, S. Curran, and D. Parry for helpful discussions. We are grateful to W. Wong and J. Suen for pouring all of the RNAi plates used in this study. This research was supported by a Helen Hay Whitney Postdoctoral Fellowship (tJ.K.K.); a Damon-Runyon Postdoctoral Fellowship, a Howard Hughes Medical Institute Fellowship, and NIH grant K08-AG21613 (tM.T.); and NIH grant GM44619 (tG.R.).