Binding of 7-methoxy-4-(aminomethyl)-coumarin to wild-type and W128F mutant cytochrome P450 2D6 studied by time-resolved fluorescence spectroscopy

Biochemical Journal

Volumn 393, Issue 3, 2006, Pages 635-643

  Stortelder, Aike ^a   Keizers, Peter H J ^a   Oostenbrink, Chris ^a   De Graaf, Chris ^a   De Kruijf, Petra ^a   Vermeulen, Nico P E ^a   Gooijer, Cees ^a   Commandeur, Jan N M ^a   Van Der Zwan, Gert ^a  

^a VU UNIVERSITY AMSTERDAM   (Netherlands)

Author keywords

Cytochrome P450; Energy transfer; Enzyme structure; Substrate binding; Time resolved fluorescence

Indexed keywords

COMPUTER SIMULATION; ENERGY TRANSFER; ENZYMES; FLUORESCENCE; MOLECULAR DYNAMICS; MUTAGENESIS; QUENCHING; SPECTROSCOPIC ANALYSIS;

CYTOCHROME P450; ENZYME STRUCTURE; SUBSTRATE BINDING; TIME-RESOLVED FLUORESCENCE;

PIGMENTS;

7 METHOXY 4 (AMINOMETHYL)COUMARIN; ACRYLAMIDE; COUMARIN DERIVATIVE; CYTOCHROME P450 2D6; HEME; IODIDE; PHENYLALANINE; TRYPTOPHAN; UNCLASSIFIED DRUG;

ANISOTROPY; ARTICLE; CALCULATION; CATALYSIS; CHEMICAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; FLUORESCENCE; FLUORESCENCE SPECTROSCOPY; MOLECULAR DYNAMICS; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; SIMULATION; STEADY STATE; STRUCTURE ANALYSIS;

AMINO ACID SUBSTITUTION; ANISOTROPY; COMPUTER SIMULATION; COUMARINS; CYTOCHROME P-450 CYP2D6; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; MODELS, MOLECULAR; PROTEIN BINDING; PROTEIN CONFORMATION; SPECTROMETRY, FLUORESCENCE;

ESCHERICHIA COLI;

EID: 31544457588     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20051169     Document Type: Article

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