Molecular diagnosis of human granulocytic anaplasmosis

Expert Review of Molecular Diagnostics

Volumn 4, Issue 4, 2004, Pages 559-569

  Dumler, J Stephen ^a   Brouqui, Philippe ^b  

^a DEPARTMENT OF PATHOLOGY   (United States)

^b AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Anaplasmataceae; Anaplasmosis; Ehrlichia; Ehrlichiosis; Molecular diagnosis; PCR; rickertsia; Tick borne infection; Zoonosis

Indexed keywords

AMINOGLYCOSIDE; AMOXICILLIN; AZITHROMYCIN; CEFTRIAXONE; CHLORAMPHENICOL; CLARITHROMYCIN; COTRIMOXAZOLE; DOXYCYCLINE; ERYTHROMYCIN; LEVOFLOXACIN; QUINOLONE DERIVATIVE; RIFAMPICIN;

ANAPLASMA PHAGOCYTOPHILUM; ANAPLASMOSIS; ANTIBIOTIC THERAPY; BLOOD; DIAGNOSTIC APPROACH ROUTE; DIAGNOSTIC TEST; DIFFERENTIAL DIAGNOSIS; EARLY DIAGNOSIS; FATALITY; FEVER; GENE SEQUENCE; GENE TARGETING; HOSPITAL PATIENT; HUMAN; HUMAN GRANULOCYTIC ANAPLASMOSIS; MOLECULAR BIOLOGY; NEUTROPHIL; NONHUMAN; NUCLEIC ACID AMPLIFICATION; REVIEW;

ANAPLASMA PHAGOCYTOPHILUM; ANAPLASMOSIS; ANIMALS; ANTI-BACTERIAL AGENTS; GRANULOCYTES; HUMANS; IXODES; MOLECULAR DIAGNOSTIC TECHNIQUES; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEROLOGIC TESTS;

EID: 3142763815     PISSN: 14737159     EISSN: None     Source Type: Journal    
DOI: 10.1586/14737159.4.4.559     Document Type: Review

References (78)

1. Bakken JS, Dumler JS. Human granulocytic ehrlichiosis. Clin. Infect. Dis. 31, 554-560 (2000). Extensive review of the largest cohort of North American patients with human granulocytic ehrlichiosis (HGA).
2. MacLeod JR, Gordon WS. Studies in tick-borne fever of sheep. I. Transmission by the tick, Ixodes ricinus, with a description of the disease produced. Parasitology 25, 273-285 (1933).
3. Stannard AA, Gribble DH, Smith RS. Equine ehrlichiosis: a disease with similarities to tick-borne fever and bovine petechial fever. Vet. Rec. 84, 149-150 (1969).
4. Dumler JS, Barbet AF, Bekker CP et al. Reorganization of genera in the families Rickettsiaceae and Anaplasmataceae in the order Rickettsiales: unification of some species of Ehrlichia with Anaplasma, Cowdria with Ehrlichia and Ehrlichia with Neorickettsia, descriptions of six new species combinations and designation of Ehrlichia equi and 'HGE agent' as subjective synonyms of Ehrlichia phagocytophila. Int. J. Syst. Evol. Microbiol. 51, 2145-2165 (2001). Describes the new taxonomic classification of members of the family Anaplasmataceae.
5. Chen SM, Dumler JS, Bakken JS, Walker DH. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32, 589-595 (1994).
6. Bakken JS, Dumler JS, Chen SM, Eckman MR, Van Etta LL, Walker DH. Human granulocytic ehrlichiosis in the upper Midwest United States. A new species emerging? JAMA 272, 212-218 (1994).
7. Bakken JS, Krueth J, Wilson-Nordskog C, Tilden RL, Asanovich K, Dumler JS. Clinical and laboratory characteristics of human granulocytic ehrlichiosis. JAMA 275, 199-205 (1996).
8. Goodman JL, Nelson C, Vitale B et al. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N. Engl. J. Med. 334, 209-215 (1996).
9. Dumler JS, Asanovich KM, Bakken JS. Genetic identity of North American Anaplasma phagocytophilum strains by pulsed-field gel electrophoresis (PFGE). J. Clin. Microbiol. (2004) (In Press).
10. Massung RF, Lee K, Mauel M, Gusa A. Characterization of the rRNA genes of Ehrlichia chaffeensis and Anaplasma phagocytophila. DNA Cell Biol. 21(8), 587-596 (2002).
11. Sumner JW, Nicholson WL, Massung RF. PCR amplification and comparison of nucleotide sequences from the groESL heat shock operon of Ehrlichia species. J. Clin. Microbiol. 35, 2087-2092 (1997).
12. Taillardat-Bisch AV, Raoult D, Drancourt M. RNA polymerase β-subunit-based phylogeny of Ehrlichia spp., Anaplasma spp., Neorickettsia spp. and Wolbachia pipientis. Int. J. Syst. Evol. Microbiol. 53, 455-458 (2003).
13. Inokuma H, Brouqui P, Drancourt M, Raoult D. Citrate synthase gene sequence: a new tool for phylogenetic analysis and identification of Ehrlichia. J. Clin. Microbiol. 39, 3031-3039 (2001).
14. Lee KN, Padmalayam I, Baumstark B, Baker SL, Massung RF. Characterization of the ftsZ gene from Ehrlichia chaffeensis, Anaplasma phagocytophilum and Rickettsia rickettsii and use as a differential PCR target. DNA Cell Biol. 22, 179-186 (2003).
15. Murphy CI, Storey JR, Recchia J et al. Major antigenic proteins of the agent of human granulocytic ehrlichiosis are encoded by members of a multigene family. Infect. Immun. 66, 3711-3718 (1998).
16. Ijdo JW, Sun W, Zhang Y, Magnarelli LA, Fikrig E. Cloning of the gene encoding the 44-kilodalton antigen of the agent of human granulocytic ehrlichiosis and characterization of the humoral response. Infect. Immun. 66, 3264-3269 (1998).
17. Zhi N, Ohashi N, Rikihisa Y et al. Cloning and expression of the 44-kilodalton major outer membrane protein gene of the human granulocytic ehrlichiosis agent and application of the recombinant protein to serodiagnosis. J. Clin. Microbiol. 36, 1666-1673 (1998).
18. Caspersen K, Park JH, Patil S, Dumler JS. Genetic variability and stability of Anaplasma phagocytophila msp2 (p44). Infect. Immun. 70, 1230-1234 (2002).
19. Storey JR, Doros-Richert LA, Gingrich-Baker C et al. Molecular cloning and sequencing of three granulocytic Ehrlichia genes encoding high-molecular-weight immunoreactive proteins. Infect. Immun. 66, 1356-1363 (1998).
20. Caturegli P, Asanovich KM, Walls JJ et al. ankA: an Ehrlichia phagocytophila group gene encoding a cytoplasmic protein antigen with ankyrin repeats. Infect. Immun. 68, 5277-5283 (2000).
21. Telford SR III, Dawson JE, Katavolos P et al. Perpetuation of the agent of human granulocytic ehrlichiosis in a deer tick-rodent cycle. Proc. Natl Acad. Sci. USA 93, 6209-6214 (1996).
22. Richter PJ Jr, Kimsey RB, Madigan JE et al. Ixodes pacificus (Acari: Ixodidae) as a vector of Ehrlichia equi (Rickettsiales. Ehrlichieae). J. Med. Entomol. 33, 1-5 (1996).
23. Cao WC, Zhao QM, Zhang PH et al. Granulocytic Ehrlichiae in Ixodes persulcatus ticks from an area in China where Lyme disease is endemic. J. Clin. Microbiol. 38, 4208-4210 (2000).
24. Herron MJ, Nelson CM, Larson J et al. Intracellular parasitism by the human granulocytic ehrlichiosis bacterium through the P-selectin ligand, PSGL-1. Science 288, 1653-1656 (2000).
25. Webster P, Ijdo JW, Chicoine LM, Fikrig E. The agent of human granulocytic ehrlichiosis resides in an endosomal compartment. J. Clin. Invest. 101, 1932-1941 (1998).
26. Mott J, Barnewall RE, Rikihisa Y. Human granulocytic ehrlichiosis agent and Ehrlichia chaffeensis reside in different cytoplasmic compartments in HL-60 cells. Infect. Immun. 67, 1368-1378 (1999).
27. Lepidi H, Bunnell JE, Martin ME et al. Comparative pathology and immunohistology associated with clinical illness after Ehrlichia phagocytophila-group infections. Am. J. Trop. Med. Hyg. 62, 29-37 (2000).
28. Martin ME, Bunnell JE, Dumler JS. Pathology, immunohistology and cytokine responses in early phases of human granulocytic ehrlichiosis in a murine model. J. Infect. Dis. 181, 374-378 (2000).
29. Martin ME, Caspersen K, Dumler JS. Immunopathology and ehrlichial propagation are regulated by interferon-γ and interleukin-10 in a murine model of human granulocytic ehrlichiosis. Am. J. Pathol. 158, 1881-1888 (2001).
30. Banerjee R, Anguita J, Roos D, Fikrig E. Cutting edge: infection by the agent of human granulocytic ehrlichiosis prevents the respiratory burst by downregulating gp91phox. J. Immunol. 164, 3946-3949 (2000).
31. Bakken JS, Aguero-Rosenfeld ME, Tilden RL et al. Serial measurements of hematologic counts during the active phase of human granulocytic ehrlichiosis. Clin. Infect. Dis. 32, 862-870 (2001).
32. Case records of the Massachusetts General Hospital. Weekly clinicopathological exercises. Case 37-2001. A 76-year-old man with fever, dyspnea, pulmonary infiltrates, pleural effusions and confusion. N. Engl. J. Med. 345, 1627-1634 (2001).
33. Remy V, Hansmann Y, DeMartino S, Christmann D, Brouqui P. Human anaplasmosis presenting as atypical pneumonitis in France. Clin. Infect. Dis. 37, 846-848 (2003).
34. Hardalo CJ, Quagliarello V, Dumler JS. Human granulocytic ehrlichiosis in Connecticut: report of a fatal case. Clin. Infect. Dis. 21, 910-914 (1995).
35. Walker DH, Dumler JS. Human monocytic and granulocytic ehrlichioses. Discovery and diagnosis of emerging tick-borne infections and the critical role of the pathologist. Arch. Pathol. Lab. Med 121, 785-791 (1997).
36. Lotric-Furlan S, Petrovec M, Zupanc TA et al. Human granulocytic ehrlichiosis in Europe: clinical and laboratory findings for four patients from Slovenia. Clin. Infect. Dis. 27, 424-428 (1998).
37. Klein MB, Nelson CM, Goodman JL. Antibiotic susceptibility of the newly cultivated agent of human granulocytic ehrlichiosis: promising activity of quinolones and rifamycins. Antimicrob. Agents Chemother. 41, 76-79 (1997).
38. Horowitz HW, Hsieh TC, Aguero-Rosenfeld ME et al. Antimicrobial susceptibility of Ehrlichia phagocytophila. Antimicrob. Agents Chemother. 45, 786-788 (2001).
39. Maurin M, Bakken JS, Dumler JS. Antibiotic susceptibilities of Anaplasma (Ehrlichia) phagocytophilum strains from various geographic areas in the United States. Antimicrob. Agents Chemother. 47, 413-415 (2003).
40. Maurin M, Abergel C, Raoult D. DNA gyrase-mediated natural resistance to fluoroquinolones in Ehrlichia spp. Antimicrob. Agents Chemother. 45, 2098-2105 (2001).
41. Buitrago MI, Ijdo JW, Rinaudo P et al. Human granulocytic ehrlichiosis during pregnancy treated successfully with rifampin. Clin. Infect. Dis. 27, 213-215 (1998).
42. Walker DH. Diagnosing human ehrlichiosis: current status and recommendations. ASM News 66, 287-290 (2000). Describes the comprehensive attempt at standardization of diagnostic methods for forms of ehrlichiosis, including HGA.
43. Maeda K, Markowitz N, Hawley RC, Ristic M, Cox D, McDade JE. Human infection with Ehrlichia canis, a leukocytic rickettsia. N. Engl. J. Med. 316, 853-856 (1987).
44. McQuiston JH, Paddock CD, Holman RC, Childs JE. The human ehrlichioses in the United States. Emerg. Infect. Dis. 5, 635-642 (1999).
45. Bakken JS, Haller I, Riddell D, Walls JJ, Dumler JS. The serological response of patients infected with the agent of human granulocytic ehrlichiosis. Clin. Infect. Dis. 34, 22-27 (2002).
46. Walls JJ, Aguero-Rosenfeld M, Bakken JS et al. Inter- and intralaboratory comparison of Ehrlichia equi and human granulocytic ehrlichiosis (HGE) agent strains for serodiagnosis of HGE by the immunofluorescent-antibody test. J. Clin. Microbiol. 37, 2968-2973 (1999).
47. Brouqui P, Salvo E, Dumler JS, Raoult D. Diagnosis of granulocytic ehrlichiosis in humans by immunofluorescence assay. Clin. Diagn. Lab. Immunol. 8, 199-202 (2001).
48. Aguero-Rosenfeld ME, Donnarumma L, Zentmaier L et al. Seroprevalence of antibodies that react with Anaplasma phagocytophila, the agent of human granulocytic ehrlichiosis, in different populations in Westchester County, New York. J. Clin. Microbiol. 40(2), 612-615 (2002).
49. Massung RF, Slater KG. Comparison of PCR assays for detection of the agent of human granulocytic ehrlichiosis, Anaplasma phagocytophilum. J. Clin. Microbiol. 41, 717-722 (2003). Describes the comparative analytical sensitivity of many published PCR methods for the amplification of Anaplasma phagocytophilum nucleic acids. Provides important details of the reactions, attributes and deficiencies of the various approaches and reasonable recommendations for those considering such an application.
50. Kolbert C. Detection of the agent of human granulocytic ehrlichiosis by PCR. In: PCR Protocols for Emerging Infectious Diseases. Persing D (Ed.), ASM Press, WA, USA, 106-111 (1996).
51. Edelman DC, Dumler JS. Evaluation of an improved PCR diagnostic assay for human granulocytic ehrlichiosis. Mol. Diagn. 1, 41-49 (1996). Only prospective analysis of PCR for A. phagocytophilum published. Results are compared with serological testing.
52. Pancholi P, Kolbert CP, Mitchell PD et al. Ixodes dammini as a potential vector of human granulocytic ehrlichiosis. J. Infect. Dis. 172, 1007-1012 (1995).
53. Petrovec M, Lotric Furlan S, Zupanc TA et al. Human disease in Europe caused by a granulocytic Ehrlichia species. J. Clin. Microbiol. 35, 1556-1559 (1997).
54. Yeh MT, Mather TN, Coughlin RT, Gingrich-Baker C, Sumner JW, Massung RF. Serologic and molecular detection of granulocytic ehrlichiosis in Rhode Island. J. Clin. Microbiol. 35, 944-947 (1997).
55. Zhi N, Rikihisa Y, Kim HY, Wormser GP, Horowitz HW. Comparison of major antigenic proteins of six strains of the human granulocytic ehrlichiosis agent by Western immunoblot analysis. J. Clin. Microbiol. 35, 2606-2611 (1997).
56. Chu FK. Rapid and sensitive PCR-based detection and differentiation of aetiologic agents of human granulocytotropic and monocytotropic ehrlichiosis. Mol. Cell. Probes 12, 93-99 (1998).
57. Comer JA, Nicholson WL, Sumner JW, Olson JG, Childs JE. Diagnosis of human ehrlichiosis by PCR assay of acute-phase serum. J. Clin. Microbiol. 37, 31-34 (1999).
58. Lotric-Furlan S, Avsic-Zupanc T, Petrovec M et el. Clinical and serological follow-up of patients with human granulocytic ehrlichiosis in Slovenia. Clin. Diagn. Lab. Immunol. 8, 899-903 (2001).
59. Grzeszczuk A, Stanczak J, Kubica-Biernat B. Serological and molecular evidence of human granulocytic ehrlichiosis focus in the Bialowieza Primeval Forest (Puszcza Bialowieska), NorthEastern Poland. Eur. J. Clin. Microbiol. Infect. Dis. 21, 6-11 (2002).
60. Krause PJ, McKay K, Thompson CA et al. Deer-associated infection study group. disease-specific diagnosis of coinfecting tickborne zoonoses: babesiosis, human granulocytic ehrlichiosis and Lyme disease. Clin. Infect. Dis. 34, 1184-1191 (2002).
61. Bjoersdorff A, Wittesjo B, Berglun J, Massung RF, Eliasson I. Human granulocytic ehrlichiosis as a common cause of tick-associated fever in Southeast Sweden: report from a prospective clinical study. Scand. J. Infect. Dis. 34, 187-191 (2002).
62. Hulinska D, Votypka J, Plch J et al. Molecular and microscopical evidence of Ehrlichia spp. and Borrelia burgdorferi sensu lato in patients, animals and ticks in the Czech Republic. New Microbiol. 25, 437-448 (2002).
63. Arnez M, Petrovec M, Lotric-Furlan S, Zupanc TA, Strle F. First European pediatric case of human granulocytic ehrlichiosis. J. Clin. Microbiol. 39, 4591-4592 (2001).
64. Petrovec M, Bidovec A, Sumner JW et al. Infection with Anaplasma phagocytophila in cervids from Slovenia: evidence of two genotypic lineages. Wien. Klin. Wochenschr. 114, 641-647 (2002).
65. Walls JJ, Caturegli P, Bakken JS, Asanovich KM, Dumler JS. Improved sensitivity of PCR for diagnosis of human granulocytic ehrlichiosis using epank1 genes of Ehrlichia phagocytophila-group ehrlichiae. J. Clin. Microbiol. 38, 354-356 (2000).
66. Magnarelli LA, Ijdo JW, Stafford KC III, Fikrig E. Infections of granulocytic Ehrlichiae and Borrelia burgdorferi in white-tailed deer in Connecticut. J. Wildl. Dis. 35, 266-274 (1999).
67. Ijdo JW, Meek JI, Cartter ML et al. The emergence of another tickborne infection in the 12-town area around Lyme, Connecticut human granulocytic ehrlichiosis. J. Infect. Dis. 181, 1388-1393 (2000).
68. Aguero-Rosenfeld ME, Kalantarpour F, Baluch M et al. Serology of culture-confirmed cases of human granulocytic ehrlichiosis. J. Clin. Microbiol. 38, 635-638 (2000).
69. Molloy PJ, Persing DH, Berardi VE. False-positive results of PCR testing for Lyme disease. Clin. Infect Dis. 33, 412-413 (2001).
70. Aguero-Rosenfeld ME, Horowitz HW, Wormser GP et al. Human granulocytic ehrlichiosis: a case series from a medical center in New York State. Ann. Intern. Med. 125, 904-908 (1996).
71. des Vignes F, Piesman J, Heffernan R et al. Effect of tick removal on transmission of Borrelia burgdorferi and Ehrlichia phagocytophila by Ixodes scapularis nymphs. J. Infect. Dis. 183, 773-778 (2001).
72. Nadelman RB, Horowitz HW, Hsieh TC et al. Simultaneous human granulocytic ehrlichiosis and Lyme borreliosis. N. Engl. J. Med. 337, 27-30 (1997).
73. Hofmeister EK, Kolbert CP, Abdulkarim AS et al. Cosegregation of a novel Bartonella species with Borrelia burgdorferi and Babesia microti in Peromyscus leucopus. J. Infect. Dis. 177, 409-416 (1998).
74. Belongia EA, Reed KD, Mitchell PD et al. Tickhorne infections as a cause of nonspecific febrile illness in Wisconsin. Clin. Infect. Dis. 32, 1434-1439 (2001).
75. Buller RS, Arens M, Hmiel SP et al. Ehrlichia ewingii etvingii, a newly recognized agent of human ehrlichiosis. N. Engl. J. Med. 341, 148-155 (1999).
76. Courtney JW, Massung RF. Multiplex Taqman PCR assay for rapid detection of Anaplasma phagocytophila and Borrelia burgdorferi. Ann. NY Acad. Sci. 990, 369-370 (2003).
77. Kulesh DA, Baker RO, Loveless BM et al. Smallpox and pan-orthopox virus detection by real-time 3′-minor groove binder TaqMan assays on the Roche LightCycler and the Cepheid Smart Cycler platforms. J. Clin. Microbiol. 42, 601-609 (2004).
78. Boulos A, Rolain JM, Maurin M, Raoult D. Measurement of the antibiotic susceptibility of Coxiella burnetii using real time PCR. Int. J. Antimicrob. Agents 23, 169-174 (2004).