Improved sensitivity of PCR for diagnosis of human granulocytic ehrlichiosis using epank1 genes of Ehrlichia phagocytophila-group Ehrlichiae

Journal of Clinical Microbiology

Volumn 38, Issue 1, 2000, Pages 354-356

  Walls, Jennifer J ^a   Caturegli, Patrizio ^a   Bakken, Johan S ^b   Asanovich, Kristin M ^a   Dumler, J Stephen ^a,c  

^a JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b SMDC Health System   (United States)

^c DEPARTMENT OF PATHOLOGY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANKYRIN; BACTERIAL ANTIGEN; RIBOSOME RNA;

ANAPLASMA PHAGOCYTOPHILUM; ANTIGEN EXPRESSION; ARTICLE; BACTERIAL GENE; BLOOD SAMPLING; CLINICAL ARTICLE; CROSS REACTION; DIAGNOSTIC VALUE; DNA FLANKING REGION; EHRLICHIOSIS; HUMAN; IMMUNOFLUORESCENCE TEST; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SEROCONVERSION;

ANKYRIN REPEAT; ANKYRINS; CROSS REACTIONS; EHRLICHIA; EHRLICHIOSIS; FALSE POSITIVE REACTIONS; GENES, BACTERIAL; GRANULOCYTES; HUMANS; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY;

EID: 0033984292     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (13)

1. Aguero-Rosenfeld, M. E., H. W. Horowitz, G. P. Wormser, D. F. McKenna, J. Nowakowski, J. Munoz, and J. S. Dumler. 1996. Human granulocytic ehrlichiosis: a case series from a single medical center in New York State. Ann. Intern. Med. 125:904-908.
2. Bakken, J. S., J. Krueth, C. Wilson-Nordskog, R. L. Tilden, K. Asanovich, and J. S. Dumler. 1996. Clinical and laboratory characteristics of human granulocytic ehrlichiosis. JAMA 275:199-205.
3. Buller, R. S., M. Arens, S. P. Hmiel, C. D. Paddock, J. W. Sumner, Y. Rikhisa, A. Unver, M. Gaudreault-Keener, F. A. Manian, A. M. Liddell, N. Schmulewitz, and G. A. Storch. 1999. Ehrlichia ewingii, a newly recognized agent of human ehrlichiosis. N. Engl. J. Med. 341:148-155.
4. Chen, S.-M., J. S. Dumler, J. S. Bakken, and D. H. Walker. 1994. Identification of a granulocytotropic Ehrlichia species as the etiologic agent of human disease. J. Clin. Microbiol. 32:589-595.
5. Dumler, J. S., and J. S. Bakken. 1995. Ehrlichial diseases of humans: emerging tick-borne infections. Clin. Infect. Dis. 20:1102-1110.
6. Dumler, J. S., K. M. Asanovich, J. S. Bakken, P. Richter, R. Kimsey, and J. E. Madigan. 1995. Serologic cross-reactions among Ehrlichia equi, Ehrlichia phagocytophila, and human granulocytic ehrlichia. J. Clin. Microbiol. 33:1098-1103.
7. Edelman, D. C., and J. S. Dumler. 1996. Evaluation of an improved PCR diagnostic assay for human granulocytic ehrlichiosis. Mol. Diagn. 1:41-49.
8. Goodman, J. L., C. Nelson, V. Blaise, J. E. Madigan, J. S. Dumler, T. J. Kurtti, and U. G. Munderloh. 1996. Direct cultivation of the causative agent of human granulocytic ehrlichiosis. N. Engl. J. Med. 334:209-215.
9. Horowitz, H. W., M. E. Aguero-Rosenfeld, D. F. McKenna, D. Holmgren, T.-C. Hsieh, S. A. Varde, J. S. Dumler, J. M. Wu, I. Schwartz, Y. Rikihisa, and G. P. Wormser. 1998. Clinical and laboratory spectrum of culture-proven human granulocytic ehrlichiosis: comparison with culture-negative cases. Clin. Infect. Dis. 27:1314-1317.
10. Magnarelli, L. A., J. W. Ijdo, J. S. Dumler, R. Heimer, and E. Fikrig. 1998. Reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays. J. Infect. Dis. 178:1835-1838.
11. Storey, J. R., L. A. Doros-Richert, C. Gingrich-Baker, K. Munroe, T. N. Mather, R. T. Coughlin, G. A. Beltz, and C. I. Murphy. 1998. Molecular cloning and sequencing of three granulocytic Ehrlichia genes encoding high-molecular-weight immunoreactive proteins. Infect. Immun. 66:1356-1363.
12. Walker, D. H., and J. S. Dumler. 1996. Emergence of the ehrlichioses as human health problems. Emerg. Infect. Dis. 2:18-29.
13. Walls, J. J., M. Aguero-Rosenfeld, J. S. Bakken, J. L. Goodman, D. Hossain, R. C. Johnson, and J. S. Dumler. Inter- and intralaboratory comparison of Ehrlichia equi and human granulocytic ehrlichiosis (HGE) agent strains for serodiagnosis of HGE by the immunofluorescent-antibody test. J. Clin. Microbiol. 37:2968-2937.