Quantification of the expression and inducibility of 12 rat cytochrome P450 isoforms by quantitative RT-PCR

Journal of Biochemical and Molecular Toxicology

Volumn 19, Issue 6, 2006, Pages 368-378

  Caron, Etienne ^a   Rioux, Nathalie ^a   Nicolas, Olivier ^a   Lebel Talbot, Hugo ^a   Hamelin, Bettina A ^a,b,c  

^a Shire Canada   (Canada)

^b UNIVERSITÉ LAVAL   (Canada)

^c PFIZER INC   (United States)

Author keywords

CYP; Induction; Rat liver; RT PCR

Indexed keywords

3 METHYLCHOLANTHRENE; CYCLOPHILIN; CYTOCHROME P450; CYTOCHROME P450 1A1; CYTOCHROME P450 1A2; CYTOCHROME P450 1B1; CYTOCHROME P450 2B1; CYTOCHROME P450 2B2; CYTOCHROME P450 2C11; CYTOCHROME P450 2C13; CYTOCHROME P450 2C6; CYTOCHROME P450 2E1; CYTOCHROME P450 3A1; CYTOCHROME P450 3A2; DEXAMETHASONE; ISOENZYME; MESSENGER RNA; PHENOBARBITAL; PYRIDINE; RIFAMPICIN; UNCLASSIFIED DRUG; XENOBIOTIC AGENT;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DNA STRAND; ENZYME INDUCTION; FLUORESCENCE ANALYSIS; MALE; NONHUMAN; NUCLEOTIDE SEQUENCE; PICOGREEN FLUORESCENCE; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; RAT; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA STRAND; RNA STRUCTURE; SENSITIVITY AND SPECIFICITY;

ANIMALIA;

EID: 31344437157     PISSN: 10956670     EISSN: 10990461     Source Type: Journal    
DOI: 10.1002/jbt.20103     Document Type: Article

References (40)

1. Danielson PB. The cytochrome P450 superfamily: biochemistry, evolution and drug metabolism in humans. Curr Drug Metab 2002;3(6):561-597.
2. Yan Z, Caldwell GW. Metabolism profiling, and cytochrome P450 inhibition & induction in drug discovery. Curr Top Med Chem 2001;1(5):403-425.
3. Meadows M. Why drugs get pulled off the market. FDA Consum 2002;36(1):11-17.
4. Rushmore TH, Kong AN. Pharmacogenomics, regulation and signaling pathways of phase I and II drug metabolizing enzymes. Curr Drug Metab 2002;3(5):481-490.
5. Wrighton SA, Vandenbranden M, Stevens JC, Shipley LA, Ring BJ, Rettie AE, Cashman JR. In vitro methods for assessing human hepatic drug metabolism: their use in drug development. Drug Metab Rev 1993;25(4):453-484.
6. Rodriguez-Antona C, Jover R, Gomez-Lechon MJ, Castell JV. Quantitative RT-PCR measurement of human cytochrome P-450s: application to drug induction studies. Arch Biochem Biophys 2000;376(1):109-116.
7. Morris DL, Davila JC. Analysis of rat cytochrome P450 isoenzyme expression using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Biochem Pharmacol 1996;52(5):781-792.
8. Bustin SA. Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 2000;25(1):169-193.
9. Bowen WP, Carey JE, Miah A, McMurray HF, Munday PW, James RS, Coleman RA, Brown AM. Measurement of cytochrome P450 gene induction in human hepatocytes using quantitative real-time reverse transcriptase-polymerase chain reaction. Drug Metab Dispos 2000;28(7):781-788.
10. Freeman WM, Walker SJ, Vrana KE. Quantitative RT-PCR: pitfalls and potential. Biotechniques 1999;26(1):112-122, 124-115.
11. Henley WN, Schuebel KE, Nielsen DA. Limitations imposed by heteroduplex formation on quantitative RT-PCR. Biochem Biophys Res Commun 1996;226(1):113-117.
12. Mahnke A, Strotkamp D, Roos PH, Hanstein WG, Chabot GG, Nef P. Expression and inducibility of cytochrome P450 3A9 (CYP3A9) and other members of the CYP3A subfamily in rat liver. Arch Biochem Biophys 1997;337(1):62-68.
13. Kim H, Putt DA, Zangar RC, Wolf CR, Guengerich FP, Edwards RJ, Hollenberg PF, Novak RF. Differential induction of rat hepatic cytochromes P450 3A1, 3A2, 2B1, 2B2, and 2E1 in response to pyridine treatment. Drug Metab Dispos 2001;29(3):353-360.
14. Moorthy B. Persistent expression of 3-methylcholanthrene-inducible cytochromes P4501A in rat hepatic and extrahepatic tissues. J Pharmacol Exp Ther 2000;294(1):313-322.
15. Schilter B, Andersen MR, Acharya C, Omiecinski CJ. Activation of cytochrome P450 gene expression in the rat brain by phenobarbital-like inducers. J Pharmacol Exp Ther 2000;294(3):916-922.
16. Olatunde Farombi E, Akinloye O, Akinmoladun CO, Emerole GO. Hepatic drug metabolizing enzyme induction and serum triacylglycerol elevation in rats treated with chlordiazepoxide, griseofulvin, rifampicin and phenytoin. Clin Chim Acta 1999;289(1-2):1-10.
17. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E. Housekeeping genes as internal standards: use and limits. J Biotechnol 1999;75(2-3):291-295.
18. Piscaglia F, Knittel T, Kobold D, Barnikol-Watanabe S, Di Rocco P, Ramadori G. Cellular localization of hepatic cytochrome 1B1 expression and its regulation by aromatic hydrocarbons and inflammatory cytokines. Biochem Pharmacol 1999;58(1):157-165.
19. Badawi AF, Cavalieri EL, Rogan EG. Effect of chlorinated hydrocarbons on expression of cytochrome P450 1A1, 1A2 and 1B1 and 2- and 4-hydroxylation of 17beta-estradiol in female Sprague-Dawley rats. Carcinogenesis 2000;21(8):1593-1599.
20. Hatanaka N, Yamazaki H, Kizu R, Hayakawa K, Aoki Y, Iwanari M, Nakajima M, Yokoi T. Induction of cytochrome P450 1B1 in lung, liver and kidney of rats exposed to diesel exhaust. Carcinogenesis 2001;22(12):2033-2038.
21. Lin JH, Lu AY. Inhibition and induction of cytochrome P450 and the clinical implications. Clin Pharmacokinet 1998;35(5):361-390.
22. Kim H, Reddy S, Novak RF. 3-Methylcholanthrene and pyridine effects on CYP1A1 and CYP1A2 expression in rat renal tissue. Drug Metab Dispos 1995;23(8):818-824.
23. Jones EJ, Riddick DS. Regulation of constitutive rat hepatic cytochromes P450 by 3-methylcholanthrene. Xenobiotica 1996;26(10):995-1012.
24. Jones CR, Guengerich FP, Rice JM, Lubet RA. Induction of various cytochromes CYP2B, CYP2C and CYP3A by phenobarbitone in non-human primates. Pharmacogenetics 1992;2(4):160-172.
25. Sakuma T, Ohtake M, Katsurayama Y, Jarukamjorn K, Nemoto N. Induction of CYP1A2 by phenobarbital in the livers of aryl hydrocarbon-responsive and - nonresponsive mice. Drug Metab Dispos 1999;27(3):379-384.
26. Pan J, Xiang Q, Ball S. Use of a novel real-time quantitative reverse transcription-polymerase chain reaction method to study the effects of cytokines on cytochrome P450 mRNA expression in mouse liver. Drug Metab Dispos 2000;28(6):709-713.
27. Kim SG, Reddy SL, States JC, Novak RF. Pyridine effects on expression and molecular regulation of the cytochrome P450IA gene subfamily. Mol Pharmacol 1991;40(1):52-57.
28. Agrawal AK, Shapiro BH. Differential expression of gender-dependent hepatic isoforms of cytochrome P-450 by pulse signals in the circulating masculine episodic growth hormone profile of the rat. J Pharmacol Exp Ther 2000;292(1):228-237.
29. Timsit YE, Riddick DS. Interference with growth hormone stimulation of hepatic cytochrome P4502C11 expression in hypophysectomized male rats by 3-methylcholanthrene. Toxicol Appl Pharmacol 2000;163(2):105-114.
30. Shapiro BH, Agrawal AK, Pampori NA. Gender differences in drug metabolism regulated by growth hormone. Int J Biochem Cell Biol 1995;27(1):9-20.
31. Gerbal-Chaloin S, Pascussi JM, Pichard-Garcia L, Daujat M, Waechter F, Fabre JM, Carrere N, Maurel P. Induction of CYP2C genes in human hepatocytes in primary culture. Drug Metab Dispos 2001;29(3):242-251.
32. Novak RF, Woodcroft KJ. The alcohol-inducible form of cytochrome P450 (CYP 2E1): role in toxicology and regulation of expression. Arch Pharm Res 2000;23(4):267-282.
33. Sumida A, Fukuen S, Yamamoto I, Matsuda H, Naohara M, Azuma J. Quantitative analysis of constitutive and inducible CYPs mRNA expression in the HepG2 cell line using reverse transcription-competitive PCR. Biochem Biophys Res Commun 2000;267(3):756-760.
34. Jones SA, Moore LB, Shenk JL, Wisely GB, Hamilton GA, McKee DD, Tomkinson NC, LeCluyse EL, Lambert MH, Willson TM and others. The pregnane X receptor: a promiscuous xenobiotic receptor that has diverged during evolution. Mol Endocrinol 2000;14(1):27-39.
35. Sampol E, Mirrione A, Villard PH, Piccerelle P, Scoma H, Berbis P, Barra Y, Durand A, Lacarelle B. Evidence for a tissue-specific induction of cutaneous CYP2E1 by dexamethasone. Biochem Biophys Res Commun 1997;235(3):557-561.
36. Hoen PA, Commandeur JN, Vermeulen NP, Van Berkel TJ, Bijsterbosch MK. Selective induction of cytochrome P450 3A1 by dexamethasone in cultured rat hepatocytes: analysis with a novel reverse transcriptase-polymerase chain reaction assay section sign. Biochem Pharmacol 2000;60(10):1509-1518.
37. Waxman DJ. Rat hepatic cytochrome P-450 isoenzyme 2c. Identification as a male-specific, developmentally induced steroid 16 alpha-hydroxylase and comparison to a female-specific cytochrome P-450 isoenzyme. J Biol Chem 1984;259(24):15481-15490.
38. Yoshihara S, Nagata K, Wada I, Yoshimura H, Kuroki H, Masuda Y. A unique change of steroid metabolism in rat liver microsomes induced with highly toxic polychlorinated biphenyl (PCB) and polychlorinated dibenzofuran (PCDF). J Pharmacobiodyn 1982;5(12):994-1004.
39. LeCluyse EL. Pregnane X receptor: molecular basis for species differences in CYP3A induction by xenobiotics. Chem Biol Interact 2001;134(3):283-289.
40. Kocarek TA, Schuetz EG, Strom SC, Fisher RA, Guzelian PS. Comparative analysis of cytochrome P4503A induction in primary cultures of rat, rabbit, and human hepatocytes. Drug Metab Dispos 1995;23(3):415-421.