Characterization of human hepatocytes isolated from non-transplantable livers

Archivum Immunologiae et Therapiae Experimentalis

Volumn 53, Issue 5, 2005, Pages 442-453

  Łaba, Anna ^a   Ostrowska, Alina ^b   Patrzałek, Dariusz ^c   Paradowski, Leszek ^c   Lange, Andrzej ^a,d  

^a INSTITUTE OF IMMUNOLOGY AND EXPERIMENTAL THERAPY   (Poland)

^b 175 May St   (United States)

^c MEDICAL UNIVERSITY OF LUBLIN   (Poland)

^d Lower Silesian Centre for Cellular Transplantation   (Poland)

Author keywords

Cryopreservation; Hepatocyte isolation; Human; Non parenchymal cells

Indexed keywords

CD20 ANTIGEN; CD3 ANTIGEN; CD34 ANTIGEN; CD45RA ANTIGEN; CD45RO ANTIGEN; CD68 ANTIGEN; CHEMOKINE RECEPTOR CXCR4; CX3C CHEMOKINE; CYCLINE; FAS ANTIGEN; HLA DR ANTIGEN; KI 67 ANTIGEN; MONOCLONAL ANTIBODY; PROTEIN BCL 2; PROTEIN P53; STROMAL CELL DERIVED FACTOR 1;

ARTICLE; CELL DAMAGE; CELL FUNCTION; CELL ISOLATION; CELL LABELING; CELL METABOLISM; CELL STRUCTURE; CELL VIABILITY; CONTAMINATION; CONTROLLED STUDY; CRYOPRESERVATION; ENERGY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; IMMUNOGENICITY; LIVER CELL; LIVER TRANSPLANTATION; PERFUSION; PROTEIN CONTENT; THAWING; UREA CYCLE;

ADENOSINE TRIPHOSPHATE; ADULT; CELL SEPARATION; CRYOPRESERVATION; CYTOCHROME P-450 ENZYME SYSTEM; FEMALE; HEPATOCYTES; HUMANS; INFANT; MALE; MIDDLE AGED; RECEPTORS, CHEMOKINE; RECEPTORS, CXCR4;

EID: 30944435841     PISSN: 0004069X     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

1. Alexandre E., Cahn M., Abadie-Viollon C., Meyer N.B., Mantion G., Cinqualbre J., David P., Jaeck D. and Richerd L. (2002): Influence of pre-, intra-, and post-operative parameters of donor liver on the outcome of isolated human hepatocytes. Cell Tissue Bank, 3, 223-233.
2. Allen K. J. and Soriano H. E. (2001): Liver cell transplantation: the road to clinical application. J. Lab. Clin. Med., 138, 298-312.
3. Altman S. A., Randers L. and Rao G. (1993): Comparison of trypan blue dye and fluorometric assays for mammalian cell viability determinations. Biotechnol. Prog., 9, 671-674.
4. Baccarani U., Sanna A., Cariani A., Sainz-Barriga M., Adani G. L., Zambito A. M., Piccolo G., Risaliti A., Nani-Costa A., Ridolfi L., Scalamogna M., Bresadola F. and Donini A. (2003): Isolation of human hepatocytes from livers rejected for liver transplantation on a national basis: results of a 2-year experience. Liver Transplant., 9, 506-512.
5. Bilir B., Guinette D., Karrer F., Kumpe D.A., Krysl J., Stephens J. McGavran L., Ostrowska A. and Durham J. (2000): Hepatocyte transplantation in acute liver failure. Liver Transpl., 6, 32-40.
6. Bumgardner G. L., Li J., Heininger M., Ferguson R. M. and Orosz C. G. (1998): In vivo immunogenicity of purified allogeneic hepatocytes in a murine hepatocyte transplant model. Transplantation, 65, 47-52.
7. Dhawan A., Mitry R. R., Hughes R. D., Lehec S., Terry C., Bansal S., Arya R., Wade J. J., Verma A., Heaton N. D., Rela M. and Mieli-Vergani G. (2004): Hepatocyte transplantation for inherited factor VII deficiency. Transplantation, 78, 1812-1814.
8. Fisher R. A., Bu D., Thompson M., Tisnado J., Prasad U., Sterling R., Posner M. and Strom S. (2000): Defining hepatocellular chimerism in a liver failure patient bridged with hepatocyte infusion. Transplantation, 69, 303-307.
9. Fisher R. L., Gandolfi A. J. and Brendel K. (2001): Human liver quality is a dominant factor in the outcome of in vitro studies. Cell Biol. Toxicol., 17, 179-189.
10. Fox I. J. and Roy-Chowdhury J. (2004): Hepatocyte transplantation. J. Hepatol., 40, 878-886.
11. Fox I. J., Roy-Chowdhury J. R., Kaufman S. S., Goerttzen T. C., Roy-Chowdhury N. R., Warkentin P. I., Dorko K., Sauter B. V. and Stom C. S. (1998): Treatment of the Crigler-Najjar syndrome type I with hepatocyte transplantation. N. Engl. J. Med., 338, 1422-1426.
12. Fu T., Guo D., Hunag X., O'Gorman m.R., Huang I., Craford S. E. and Soriano H. E. (2001): Apoptosis occurs in isolated and banked primary mouse hepatocytes. Cell Transplant., 10, 59-66.
13. Garcia M., Rager J., Wang Q., Strab R., Hidalgo I. J., Owen A. and Li J. (2003): Cryopreserved human hepatocytes as alternative in vitro model for cytochrome p450 induction studies. In Vitro Cell Dev. Biol. Anim., 39, 283-287.
14. Gebhardt R., Hengstler J. G., Muller D., Glockner R., Buenning P., Laube B., Schmelzer E., Ullrich M., Utesch D., Hewitt N., Ringel M., Hilz B. R., Bader A., Langsch A., Koose T., Burger H. J., Maas J. and Oesch F. (2003): New hepatocyte in vitro systems for drug metabolism: metabolic capacity and recommendations for application in basic research and drug development, standard operating procedures. Drug Metab. Rev., 35, 145-213.
15. Gerlach J. C., Mutig K., Sauer I. M., Schrade P., Efimova E., Mieder T., Naumann G., Grunwald A., Pless G., Mass A., Bachmann S., Neuhaus P. and Zeilinger K. (2003): Use of primary human liver cells originating from discarded grafts in a bioreactor for liver support therapy and the prospects of culturing adult liver stem cells in bioreactors: a morphologic study. Transplantation, 76, 781-786.
16. Ghobrial I. M., Bone N. D., Stenson M. J., Novak A., Hedin K. E., Kay N. E. and Ansell S. M. (2004): Expression of the chemokine receptors CXCR4 and CCR7 and disease progression in B-cell chronic lymphocytic leukemia/small lymphocytic lymphoma. Mayo Clin. Proc., 79, 318-325.
17. Gomez-Lechon M. J., Donato M. T., Castell J. V. and Jover R. (2004): Human hepatocytes in primary culture: the choice to investigate drug metabolism in man. Curr. Drug Metab., 5, 443-462.
18. Grossmann J. (2002): Molecular mechanisms of "detachment-induced apoptosis - Anoikis". Apoptosis, 7, 247-260.
19. Gupta S. and Chowdhury J. R. (2002): Therapeutic potential of hepatocyte transplantation. Semin. Cell Dev. Biol., 13, 439-446.
20. Hengstler J. G., Utesch D., Steinberg P., Platt K. L., Diener B., Ringel M., Swales N., fischer T., Biefang K., Gerl M., Bottger T. and Oesch F. (2000): Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction. Drug Metab. Rev., 32, 81-118.
21. Horslen S. P., McCowan T. C., Goertzen T. C., Warkentin P. I., Cai H. B., Strom S. C. and Fox I. J. (2003): Isolated hepatocyte transplantation in an infant with a severe urea cycle disorder. Pediatrics, 111, 1262-1267.
22. Jauregui H. O., Chowdhury N. R., and Chowdhury J. R. (1996): Use of mammalian liver cells for artificial liver support. Cell Transplant., 5, 353-367.
23. Karrer F. M., Bilir B., Gill R., Ostrowska A., Stegall M., Wachs M. and Kam I. (1997): Cryopreserved hepatocytes are poor stimulators of in vitro cytotoxicity. Transplant. Proc., 29, 1111-1112.
24. Kobayashi N., Okitsu T. and Tanaka N. (2003): Cell choice for bioartificial livers. Keio J. Med., 52, 151-157.
25. Kollet O., Shivtiel S., Chen Y. Q., Suriawinata J., Thung S. N., Dabeva M. D., Kahn J., Spiegel A., Dar A., Samira S., Goichberg P., Kalinkovich A., Arenzana-Seisdedos F., Nagler A., Hardan I., Revel M., Shafritz D. A., and Lapidot T. (2003): HGF, SDF-1 and MMP-9 are involved in stress-induced human CD34+ stem cell recruitment to the liver. J. Clin. Invest., 112, 160-169.
26. Li A. P. (2004): In vitro approaches to evaluate ADMET drug properties. Curr. Top. Med. Chem., 4, 701-706.
27. Li A. P., Maurel P., Gomez-Lechon M. J., Cheng L. C. and Jurima-Romet M. (1997): Preclinical evaluation of drug-drug interactions: present status of the application of primary human hepatocytes in the evaluation of cytochrome P450 induction. Chem. Biol. Interact., 107, 5-16.
28. Matsubara T., Koike M., Touchi A., Tochino Y. and Sugeno K. (1976): Quantitative determination of cytochrome P-450 in rat liver homogenate. Anal. Biochem., 75, 596-603.
29. McKay G. C., Henderson C., Goldie E., Connel G., Westmorland C. and Grnat M. H. (2002): Cryopreservation of rat hepatocyte monolayers: cell viability and cytochrome P450 content in post-thaw cultures. Toxicol. In Vitro, 16, 71-79.
30. Mitry R. R., Dhawan A., Hughes R. D., Bansal S., Lehec S., Terry C., Heaton N. D., Karani J. B., Mieli-Vergani G. and Rela M. (2004): One liver, three recipients: segment IV from split-liver procedures as a source of hepatocytes for cell transplantation. Transplantation, 77, 1614-1616.
31. Mitry R. R., Hughes R. D., Aw M. M., Terry C., Mieli-Vergani G., Girlanda R., Muiesan P., Rela M., Heaton N. D. and Dhawan A. (2003): Human hepatocyte isolation and relationship of cell viability to early graft function. Cell Transplant., 12, 69-74.
32. Olinga P., Maring J. K., Merema M., Hof I. H., Slooff M., Meijer D. K. and Groothuis G. M. (2000): The capability of isolated hepatocytes and liver slices of donor livers to predict graft function after liver transplantation. Liver, 20, 374-380.
33. Olinga P., Merema M., Hof I. H., de Jong K. P., Slooff M., Meijer D. K. and Groothuis G. M. (1998): Effect of human liver source on the functionality of isolated hepatocytes and liver slices. Drug Metab. Dispos., 26, 5-11.
34. Olinga P., Merema M., Slooff M. J., Meijer D. K. and Groothuis G. M. (1997): Influence of 48 hours of cold storage in University of Wisconsin organ preservation solution on metabolic capacity of rat hepatocytes. J. Hepatol., 27, 738-743.
35. Olszewski W. L., Rudowska A. and Mecner B. (2002): Autologous transplanted hepatocytes: depletion of recipient leukocytes prevents in vivo lysis. Transplant. Proc., 34, 705-706.
36. Ostrowska A., Bode D. C., Pruss J., Bilir B., Smith G. D. and Zeisloft S. (2000): Investigation of functional and morphological integrity of freshly isolated and cryopreserved human hepatocytes. Cell Tissue Bank, 1, 55-68.
37. Ostrowska A., Karrer F. M. and Bilir B. M. (1999): Histological identification of purified and cryopreserved allogeneic hepatocytes following transplantation in a murine model without host immunosuppression. Transpl. Int., 12, 188-194.
38. Prince M. I. and Hudson M. (2002): Liver transplantation for chronic liver disease: advances and controversies in an era of organ shortages. Postgrad. Med. J., 78, 135-141.
39. Raucy J. L., Mueller L., Duan K., Allen S.W., Strom S. and Lasker J. M. (2002): Expression and induction of CYP2C P450 enzymes in primary cultures of human hepatocytes. J. Pharmacol. Exp. Ther., 302, 475-482.
40. Roymans D., Van Looveren C., Leone A., Parker J. B., McMillian M., Johnson M. D., Koganti A., Gilissen R., Silber P., Mannens G. and Meuldermans W. (2004): Determination of cytochrome P450 1A2 and cytochrome P450 3A4 induction in cryopreserved human hepatocytes. Biochem. Pharmacol., 67, 427-437.
41. Sokal E. M., Smets F., Bourgois A., Van Maldergem L., Buts J. P., Reding R., Bernard Otte J., Evrard V., Latinne D., Vincent M. F., Moser A. and Soriano H. E. (2003): Hepatocyte transplantation in a 4-year-old girl with peroxisomal biogenesis disease: Technique, safety, and metabolic follow-up. Transplantation, 76, 735-738.
42. Sriwatanawongsa V., Davies H. S. and Calne R. Y. (1995): The essential roles of parenchymal tissues and passenger leukocytes in the tolerance induced by liver grafting in rats. Nat. Med., 1, 428-432.
43. Strain A. J., Crosby H. A., Nijjar S., Kelly D. A. and Hubscher S. G. (2003): Human liver-derived stem cells. Semin. Liver Dis., 23, 373-384.
44. Strom S. C., Fisher R. A., Thompson M. T., Sanyal A. J., Cole P. E., Ham J. M. and Posner M. P. (1997): Hepatocyte transplantation as a bridge to orthotopic liver transplantation in terminal liver failure. Transplantation, 63, 559-569.
45. Walldorf J., Aurich H., Cai H., Runge D., Christ B, Strom S. C. and Fleig W. E. (2004): Expanding hepatocytes in vitro before cell transplantation: donor age-dependent proliferative capacity of cultured human hepatocytes. Scand. J. Gastroenterol., 39, 584-593.
46. Wesolowska A., Olszewski W. L. and Durlik M. (2003): Transplantation of hepatocytes: elimination of recipient natural killer cells with irradiation and bone marrow reconstitution prevent early graft dysfunction. Transplant. Proc., 35, 2358-2360.
47. Yagi T., Hardin J. A., Valenzuela Y. M., Miyoshi H., Gores G. J. and Nyberg S. L. (2001): Caspase inhibition reduces apoptotic death of cryopreserved porcine hepatocytes. Hepatology, 33, 1432-1440.
48. Yoo-Ott K. A., Schiller H., Fändrich F., Oswald H., Richter K., Xhu X. F., Kampen W. U., Krönke M. and Zavazava N. (2000): Co-transplantation of donor derived hepatocytes induces long-term tolerance to cardiac allografts in a rat model. Transplantation, 69, 2538-2546.