Identification of protein associations in organelles, using mass spectrometry-based proteomics

Mass Spectrometry Reviews

Volumn 23, Issue 4, 2004, Pages 259-280

  Warnock, Dale E ^a   Fahy, Eoin ^a   Taylor, Steven W ^a,b  

^a Migenix Inc   (United States)

^b AMYLIN PHARMACEUTICALS INC   (United States)

Author keywords

Bioinformatics; Organelle; Protein complexes; Protein protein interactions; Proteomics

Indexed keywords

BIOINFORMATICS; ORGANELLE; PROTEIN COMPLEX; PROTEIN-PROTEIN INTERACTION; PROTEOMICS;

BIOCHEMICAL ENGINEERING; BIOLOGICAL ORGANS; CELLS; DESORPTION; DNA; IONIZATION; LASER BEAM EFFECTS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY;

PROTEINS;

PROTEOME;

ANIMAL; CELL ORGANELLE; CHEMISTRY; HUMAN; MASS SPECTROMETRY; METHODOLOGY; PROTEOMICS; REVIEW;

ANIMALS; HUMANS; MASS SPECTROMETRY; ORGANELLES; PROTEOME; PROTEOMICS;

EID: 3042687234     PISSN: 02777037     EISSN: None     Source Type: Journal    
DOI: 10.1002/mas.10077     Document Type: Review

References (108)

1. Aggeler R, Coons J, Taylor SW, Ghosh SS, Garcia JJ, Capaldi RA, Marusich MF 2002. A functionally active human FIFO ATPase can be purified by immunocapture from heart tissue and fibroblast cell lines. Subunit structure and activity studies. J Biol Chem 277:33906-33912.
2. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson JD. 1994. Molecular biology of the cell. 3rd edition. New York and London: Garland Publishing.
3. Alien NP, Huang L, Burlingame A, Rexach M. 2001. Proteomic analysis of nucleoporin interacting proteins. J Biol Chem 276:29268-29274.
4. Alien NP, Patel SS, Huang L, Chalkley RJ, Burlingame A, Lutzmann M, Hurt EC, Rexach M. 2002. Deciphering networks of protein interactions at the nuclear pore complex. Mol Cell Proteomics 1:930-946.
5. Andersen JS, Lyon CE, Fox AH, Leung AK, Lam YW, Steen H, Mann M, Lamond AI. 2002. Directed proteomic analysis of the human nucleolus. Curr Biol 12:1 -11.
6. Bader GD, Betel D, Hogue CW. 2003. BIND: The biomolecular interaction network database. Nucleic Acids Res 31:248-250.
7. Bagshaw RD, Pasternak SH, Mahuran DJ, Callahan JW. 2003. Nicastrin is a resident lysosomal membrane protein. Biochem Biophys Res Commun 300:615-618.
8. Bairoch A, Apweiler R. 1997. The SWISS-PROT protein sequence data bank and its supplement TrEMBL. Nucleic Acids Res 25:31-36.
9. Balmer Y, Koller A, del Val G, Manieri W, Schurmann P, Buchanan BB. 2003. Proteomics gives insight into the regulatory function of chloroplast thioredoxins. Proc Natl Acad Sci USA 100:370-375.
10. Bannai H, Tamada Y, Maruyama O, Nakai K, Miyano S. 2002. Extensive feature detection of N-terminal protein sorting signals. Bioinformatics 18:298-305.
11. Basrur V, Yang F, Kushimoto T, Higashimoto Y, Yasumoto K, Valencia J, Muller J, Vieira WD, Watabe H, Shabanowitz J, Hearing VJ, Hunt DF, Appella E. 2003. Proteomic analysis of early melanosomes: Identification of novel melanosomal proteins. J Proteome Res 2:69-79.
12. Breitkreutz BJ, Stark C, Tyers M. 2003. The GRID: The general repository for interaction datasets. Genome Biol 4:R23.
13. Bykova NV, Egsgaard H, Moller IM. 2003. Identification of 14 new phosphoproteins involved in important plant mitochondrial processes. FEES Lett 540:141-146.
14. Carroll J, Shannon RJ, Fearnley IM, Walker JE, Hirst J. 2002. Definition of the nuclear encoded protein composition of bovine heart mitochondrial complex I. Identification of two new subunits. J Biol Chem 277:50311 -50317.
15. Carroll J, Fearnley IM, Shannon RJ, Hirst J, Walker JE. 2003. Analysis of the subunit composition of complex I from bovine heart mitochondria. Mol Cell Proteomics 2:117-126.
16. Carty SM, Greenleaf AL. 2002. Hyperphosphorylated C-terminal repeat domain-associating proteins in the nuclear proteome link transcription to DNA/chromatin modification and RNA processing. Mol Cell Proteomics 1:598-610.
17. Cavdar Koc E, Burkhart W, Blackburn K, Moseley A, Spremulli LL. 2001. The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present. J Biol Chem 276:19363-19374.
18. Chinnery PF, Howell N, Andrews RM, Turnbull DM. 1999. Clinical mitochondrial genetics. J Med Genet 36:425-436.
19. Claros MG, Vincens P. 1996. Computational method to predict mitochondrially imported proteins and their targeting sequences. Eur J Biochem 241:779-786.
20. Cokol M, Nair R, Rost B. 2000. Finding nuclear localization signals. EMBO Rep 1:411-415.
21. Da Cruz S, Xenarios I, Langridge J, Vilbois F, Parone PA, Martinou JC. 2003. Proteomic analysis of the mouse liver mitochondrial inner membrane. J Biol Chem 278:41566-41571.
22. Dermine JF, Duclos S, Garin J, St. Louis F, Rea S, Parton RG, Desjardins M. 2001. Flotillin-1-enriched lipid raft domains accumulate on maturing phagosomes. J Biol Chem 276: 18507-18512.
23. Desjardins M. 2003. ER-mediated phagocytosis: A new membrane for new functions. Nat Rev Immunol 3:280-291.
24. Desjardins M, Huber LA, Parton RG, Griffiths G. 1994. Biogenesis of phagolysosomes proceeds through a sequential series of interactions with the endocytic apparatus. J Cell Biol 124:677-688.
25. Dreger M. 2003. Subcellular proteomics. Mass Spectrom Rev 22:27-56.
26. Ducret A, Van Oostveen I, Eng JK, Yates JR III, Aebersold R. 1998. High throughput protein characterization by automated reverse-phase chromatography/electrospray tandem mass spectrometry. Protein Sei 7:706-719.
27. Eberle HB, Serrano RL, Fullekrug J, Schlosser A, Lehmann WD, Lottspeich F, Kaloyanova D, Wieland FT, Helms JB. 2002. Identification and characterization of a novel human plant pathogenesis-related protein that localizes to lipid-enriched microdomains in the Golgi complex. J Cell Sei 115:827-838.
28. Edvardsson U, Brockenhuus Von Lowenhielm H, Panfilov O, Nystrom AC, Nilsson F, Dahllof B. 2003. Hepatic protein expression of lean mice and obese diabetic mice treated with peroxisome proliferator-activated receptor activators. Proteomics 3:468-478.
29. Emanuelsson O, Nielsen H, von Heijne G. 1999. ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites, Protein Sei 8:978-984.
30. Emanuelsson O, Nielsen H, Brunak S, von Heijne G. 2000. Predicting subcellular localization of proteins based on their N-terminal amino acid sequence. J Mol Biol 300:1005-1016.
31. Fearnley IM, Carroll J, Shannon RJ, Runswick MJ, Walker JE, Hirst J. 2001. GRIM-19, a cell death regulatory gene product, is a subunit of bovine mitochondrial NADH:ubiquinone oxidoreductase (complex I). J Biol Chem 276: 38345-38348.
32. Ferro M, Salvi D, Brugiere S, Miras S, Kowalski S, Louwagie M, Garin J, Joyard J, Rolland N. 2003. Proteomics of the chloroplast envelope membranes from Arabidopsis thaliana. Mol Cell Proteomics 2:325-345.
33. Field HI, Fenyo D, Beavis RC. 2002. RADARS, a bioinformatics solution that automates proteome mass spectral analysis, optimises protein identification, and archives data in a relational database. Proteomics 2:36-47.
34. Foster LJ, De Hoog CL, Mann M. 2003. Unbiased quantitative proteomics of lipid rafts reveals high specificity for signaling factors. Proc Natl Acad Sei USA 100:5813-5818.
35. Fountoulakis M, Schlaeger EJ. 2003, The mitochondrial proteins of the neuroblastoma cell line IMR-32. Electrophoresis 24:260-275.
36. Froehlich JE, Wilkerson CGW, Ray K, McAndrew RS, Osteryoung KW, Gage DA, Phinney BS. 2003. Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis. J Proteome Res 2:413-425.
37. Gagne JP, Hunter JM, Labrecque B, Chabot B, Poirier GG. 2003. A proteomic approach to the identification of heterogeneous nuclear ribonucleoproteins as a new family of poly(ADP-ribose)-binding proteins. Biochem J 371:331-340.
38. Gagnon E, Duclos S, Rondeau C, Chevet E, Cameron PH, Steele-Mortimer O, Paiement J, Bergeron JJ, Desjardins M. 2002. Endoplasmic reticulum-mediated phagocytosis is a mechanism of entry into macrophages. Cell 110:119-131.
39. Gan X, Kitakawa M, Yoshino K, Oshiro N, Yonezawa K, Isono K. 2002. Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components. Eur J Biochem 269:5203-5214.
40. Garin J, Diez R, Kieffer S, Dermine JF, Duclos S, Gagnon E, Sadoul R, Rondeau C, Desjardins M. 2001. The phagosome proteome: Insight into phagosome functions. J Cell Biol 152:165-180.
41. Greenamyre JT, Sherer TB, Betarbet R, Panov AV. 2001. Complex I and Parkinson's disease. IUBMB Life 52:135-141.
42. Guo W, Sacher M, Barrowman J, Ferro-Novick S, Novick P. 2000. Protein complexes in transport vesicle targeting. Trends Cell Biol 10:251-255.
43. Gygi SP, Rist B, Gerber SA, Turecek F, Gelb MH, Aebersold R. 1999. Quantitative analysis of complex protein mixtures using isotope-coded affinity tags. Nat Biotechnol 17:994-999.
44. Hanson BJ, Schulenberg B, Patton WF, Capaldi RA. 2001. A novel subfractionation approach for mitochondrial proteins: A three-dimensional mitochondrial proteome map. Electrophoresis 22:950-959.
45. Harding C, Heuser J, Stahl P. 1984. Endocytosis and intracellular processing of transferrin and colloidal gold-transferrin in rat reticulocytes: Demonstration of a pathway for receptor shedding. Eur J Cell Biol 35:256-263.
46. Heazlewood JL, Howell KA, Millar AH. 2003a. Mitochondrial complex I from Arabidopsis and rice: Orthologs of mammalian and fungal components coupled with plant-specific subunits. Biochim Biophys Acta 1604:159-169.
47. Heazlewood JL, Whelan J, Millar AH. 2003c. The products of the mitochondrial orf25 and orfB genes are FO components in the plant FIFO ATP synthase. FEES Lett 540:201-215.
48. Heazlewood JL, Howell KA, Whelan J, Millar AH. 2003b. Towards an analysis of the rice mitochondrial proteome. Plant Physiol 132:230-242.
49. Herald VL, Heazlewood JL, Day DA, Millar AH. 2003. Proteomic identification of divalent metal cation binding proteins in plant mitochondria. FEES Lett 537:96-100.
50. Howell KE, Palade GE. 1982. Hepatic Golgi fractions resolved into membrane and content subfractions. J Cell Biol 92: 822-832.
51. Huber LA, Pfaller K, Victor I. 2003. Organelle proteomics: Implications for subcellular fractionation in proteomics. Circ Res 92:962-968.
52. Johnson KL, Loegering DA, Gleich GJ, Naylor S. 2003. A modular on-line three-dimensional liquid chromatography-tandem mass spectrometry approach to characterization of organelle proteomes, Biomed Chromatogr 17:106-112.
53. Journet A, Chapel A, Kieffer S, Roux F, Garin J. 2002. Proteomic analysis of human lysosomes: Application to monocytic and breast cancer cells. Proteomics 2:1026-1040.
54. Kawasaki N, Itoh S, Ohta M, Hayakawa T. 2003. Microanalysis of N-linked oligosaccharides in a glycoprotein by capillary liquid chromatography/mass spectrometry and liquid chromatography/tandem mass spectrometry. Anal Biochem 316:15-22.
55. Koc EC, Burkhart W, Blackburn K, Moyer MB, Schlatzer DM, Moseley A, Spremulli LL. 2001. The large subunit of the mammalian mitochondrial ribosome. Analysis of the complement of ribosomal proteins present. J Biol Chem 276: 43958-43969.
56. Koo AJ, Ohlrogge JB. 2002. The predicted candidates of Arabidopsis plastid inner envelope membrane proteins and their expression profiles. Plant Physiol 130:823-836.
57. Krogh A, Larsson B, von Heijne G, Sonnhammer EL. 2001. Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes. J Mol Biol 305:567-580.
58. Kumar A, Agarwal S, Heyman JA, Matson S, Heidtman M, Piccirillo S, Umansky L, Drawid A, Jansen R, Liu Y, Cheung KH, Miller P, Gerstein M, Roeder GS, Snyder M. 2002. Subcellular localization of the yeast proteome. Genes Dev 16:707-719.
59. Lescuyer P, Strub JM, Luche S, Diemer H, Martinez P, Van Dorsselaer A, Lunardi J, Rabilloud T. 2003. Progress in the definition of a reference human mitochondrial proteome. Proteomics 3:157-167.
60. Li W, Jaroszewski L, Godzik A. 2001. Clustering of highly homologous sequences to reduce the size of large protein databases. Bioinformatics 17:282-283.
61. Li N, Mak A, Richards DP, Naber C, Keller BO, Li L, Shaw AR. 2003. Monocyte lipid rafts contain proteins implicated in vesicular trafficking and phagosome formation. Proteomics 3:536-548.
62. Lill J. 2003. Proteomic tools for quantitation by mass spectrometry. Mass Spectrom Rev 22:182-194.
63. Link AJ, Eng J, Schieltz DM, Carmack E, Mize GJ, Morris DR, Garvik BM, Yates JR. 1999. Direct analysis of protein complexes using mass spectrometry. Nat Biotechnol 17: 676-682.
64. Marcotte EM, Pellegrini M, Thompson MJ, Yeates TO, Eisenberg D. 1999. A combined algorithm for genomewide prediction of protein function. Nature 402:83-86.
65. Millar AH, Heazlewood JL. 2003. Genomic and proteomic analysis of mitochondrial carrier proteins in Arabidopsis. Plant Physiol 131:443-453.
66. Murray J, Taylor SW, Zhang B, Ghosh SS, Capaldi RA. 2003a. Oxidative damage to mitochondrial complex I due to peroxynitrite: Identification of reactive tyrosines by mass spectrometry. J Biol Chem 278:37223-37230.
67. Murray J, Zhang B, Taylor SW, Oglesbee D, Fahy E, Marusich MF, Ghosh SS, Capaldi RA. 2003b. The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification. J Biol Chem 278:13619-13622.
68. Nakai K, Horton P. 1999. PSORT: A program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem Sci 24:34-36.
69. Nakai K, Kanehisa M. 1992. A knowledge base for predicting protein localization sites in eukaryotic cells. Genomics 14:897-911.
70. Ni L, Heard TS, Weiner H. 1999. In vivo mitochondrial import. A comparison of leader sequence charge and structural relationships with the in vitro model resulting in evidence for co-translational import. J Biol Chem 274:12685-12691.
71. Nielsen H, Engelbrecht J, Brunak S, von Heijne G. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng 10:1-6.
72. Orchard S, Kersey P, Zhu W, Montecchi-Palazzi L, Hermjakob H, Apweiler R. 2003. Progress in establishing common standards for exchanging proteomics data: The second meeting of the HUPO Proteomics Standards Initiative. Comp Funct Genomics 4:203-206.
73. Panigrahi AK, Alien TE, Stuart K, Haynes PA, Gygi SP. 2003. Mass spectrometric analysis of the editosome and other multiprotein complexes in Trypanosoma brucei. J Am Soc Mass Spectrom 14:728-735.
74. Pasternak SH, Bagshaw RD, Guiral M, Zhang S, Ackerley CA, Pak BJ, Callahan JW, Mahuran DJ. 2003. Presenilin-1, nicastrin, amyloid precursor protein, and gamma-secretase activity are co-localized in the lysosomal membrane. J Biol Chem 278:26687-26694.
75. Peng J, Elias JE, Thoreen CC, Licklider LJ, Gygi SP. 2003. Evaluation of multidimensional chromatography coupled with tandem mass spectrometry (LC/LC-MS/MS) for large-scale protein analysis: The yeast proteome. J Proteome Res 2:43-50.
76. Pitt A, Mayorga LS, Stahl PD, Schwartz AL. 1992. Alterations in the protein composition of maturing phagosomes. J Clin Invest 90:1978-1983.
77. Pruitt KD, Katz KS, Sicotte H, Maglott DR. 2000. Introducing RefSeq and LocusLink: Curated human genome resources at the NCBI. Trends Genet 16:44-47.
78. Ranish JA, Yi EC, Leslie DM, Purvine SO, Goodlett DR, Eng J, Aebersold R. 2003. The study of macromolecular complexes by quantitative proteomics. Nat Genet 33:349-355.
79. Raposo G, Fevrier B, Stoorvogel W, Marks MS. 2002. Lysosome-related organelles: A view from immunity and pigmentation. Cell Struct Funct 27:443-456.
80. Richly E, Chinnery PF, Leister D. 2003. Evolutionary diversification of mitochondrial proteomes: Implications for human disease. Trends Genet 19:356-362.
81. Rojo E, Zouhar J, Carter C, Kovaleva V, Raikhel NV. 2003. A unique mechanism for protein processing and degradation in Arabidopsis thaliana. Proc Natl Acad Sci USA 100:7389-7394.
82. Scheffler NK, Miller SW, Carroll AK, Anderson C, Davis RE, Ghosh SS, Gibson BW. 2001. Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line. Mitochondrion 1:161-179.
83. Scherl A, Coute Y, Deon C, Calle A, Kindbeiter K, Sanchez JC, Greco A, Hochstrasser D, Diaz JJ. 2002. Functional proteomic analysis of human nucleolus. Mol Biol Cell 13:4100-4109.
84. Schulenberg B, Aggeler R, Beechem JM, Capaldi RA, Patton WF. 2003. Analysis of steady-state protein phosphorylation in mitochondria using a novel fluorescent phosphosensor dye. J Biol Chem 278:27251-27255.
85. Suzuki T, Terasaki M, Takemoto-Hori C, Hanada T, Ueda T, Wada A, Watanabe K. 2001 a. Proteomic analysis of the mammalian mitochondrial ribosome - Identification of protein components in the 28 S small subunit. J Biol Chem 276:33181-33195.
86. Suzuki T, Terasaki M, Takemoto-Hori C, Hanada T, Ueda T, Wada A, Watanabe K. 200 Ib. Structural compensation for the deficit of rRNA with proteins in the mammalian mitochondrial ribosome. Systematic analysis of protein components of the large ribosomal subunit from mammalian mitochondria. J Biol Chem 276:21724-21736.
87. Taylor SW, Fahy E, Ghosh SS. 2003a. Global organellar proteomics. Trends Biotechnol 21:82-88.
88. Taylor RS, Jones SM, Dahl RH, Nordeen MH, Howell KE. 1997. Characterization of the Golgi complex cleared of proteins in transit and examination of calcium uptake activities. Mol Biol Cell 8:1911-1931.
89. Taylor RS, Wu CC, Hays LG, Eng JK, Yates JR III, Howell KE. 2000. Proteomics of rat liver Golgi complex: Minor proteins are identified through sequential fractionation. Electrophoresis 21:3441-3459.
90. Taylor SW, Warnock DE, Glenn GM, Zhang B, Fahy E, Gaucher SP, Capaldi RA, Gibson BW, Ghosh SS. 2002. An alternative strategy to determine the mitochondrial proteome using sucrose gradient fractionation and ID PAGE on highly purified human heart mitochondria. J Proteome Res 1:451 -458.
91. Taylor SW, Fahy E, Murray J, Capaldi RA, Ghosh SS. 2003b. Oxidative post-translational modification of tryptophan residues in cardiac mitochondrial proteins. J Biol Chem 278:19587-19590.
92. Taylor SW, Fahy E, Zhang B, Glenn GM, Warnock DE, Wiley S, Murphy AN, Gaucher SP, Capaldi RA, Gibson BW, Ghosh SS. 2003c. Characterization of the human heart mitochondrial proteome. Nat Biotechnol 21:281-286.
93. The Arabidopsis Initiative. 2000. Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796-815.
94. Thery C, Zitvogel L, Amigorena S. 2002. Exosomes: Composition, biogenesis, and function. Nat Rev Immunol 2:569-579.
95. Volkl A, Mohr H, Fahimi HD. 1999. Peroxisome subpopulations of the rat liver. Isolation by immune free flow electrophoresis. J Histochem Cytochem 47:1111 -1118.
96. White IR, Man WJ, Bryant D, Bugelski P, Camilleri P, Cutler P, Hayes W, Holbrook JD, Kramer K, Lord PG, Wood J. 2003. Protein expression changes in the Sprague-Dawley rat liver proteome following administration of peroxisome proliferator activated receptor alpha and gamma ligands. Proteomics 3:505-512.
97. Whitelegge JP, Zhang H, Aguilera R, Taylor RM, Cramer WA. 2002. Full subunit coverage liquid chromatography electrospray ionization mass spectrometry (LCMS+) of an oligomeric membrane protein: Cytochrome b(6)f complex from Spinach and the Cyanobacterium mastigocladus laminosus. Mol Cell Proteomics 1:816-827.
98. Whyte JR, Munro S. 2002. Vesicle tethering complexes in membrane traffic. J Cell Sci 115:2627-2637.
99. Wu CC, MacCoss MJ, Howell KE, Yates JR. 2003. A method for the comprehensive proteomic analysis of membrane proteins. Nat Biotechnol 21:532-538.
100. Wubbolts R, Leckie RS, Veenhuizen PT, Schwarzmann G, Mobius W, Hoernschemeyer J, Slot JW, Geuze HJ, Stoorvogel W. 2003. Proteomic and biochemical analyses of human B cell-derived exosomes. Potential implications for their function and multivesicular body formation. J Biol Chem 278:10963-10972.
101. Xenarios I, Salwinski L, Duan XJ, Higney P, Kim SM, Eisenberg D. 2002. DIP, the database of interacting proteins: A research tool for studying cellular networks of protein interactions. Nucleic Acids Res 30:303-305.
102. Yamaguchi K, Subramanian AR. 2003. Proteomic identification of all plastid-specific ribosomal proteins in higher plant chloroplast 30S ribosomal subunit. Eur J Biochem 270:190-205.
103. Yamaguchi K, Prieto S, Beligni MV, Haynes PA, McDonald WH, Yates JR III, Mayfield SP. 2002. Proteomic characterization of the small subunit of Chlamydomonas reinhardtii chloroplast ribosome: Identification of a novel S1 domaincontaining protein and unusually large orthologs of bacterial S2, S3, and S5. Plant Cell 14:2957-2974.
104. Yamaguchi K, Beligni MV, Prieto S, Haynes PA, McDonald WH, Yates JR III, Mayfield SP. 2003. Proteomic characterization of the Chlamydomonas reinhardtii chloroplast ribosome: Identification of proteins unique to the 70S ribosome. J Biol Chem 278:33774-33785.
105. Zanzoni A, Montecchi-Palazzi L, Quondam M, Ausiello G, Helmer-Citterich M, Cesareni G. 2002. MINT: A molecular interaction database. FEBS Lett 513:135-140.
106. Zischka H, Weber G, Weber PJ, Posch A, Braun RJ, Buhringer D, Schneider U, Nissum M, Meitinger T, Ueffing M, Eckerskorn C. 2003. Improved proteome analysis of Saccharomyces cerevisiae mitochondria by free-flow electrophoresis. Proteomics 3:906-916.
107. Zolla L, Rinalducci S, Timperio AM, Huber CG. 2002. Proteomics of light-harvesting proteins in different plant species. Analysis and comparison by liquid chromatography-electrospray ionization mass spectrometry. Photosystem I. Plant Physiol 130:1938-1950.
108. Zolla L, Timperio AM, Walcher W, Huber CG. 2003. Proteomics of light-harvesting proteins in different plant species. Analysis and comparison by liquid chromatography-electrospray ionization mass spectrometry. Photosystem II. Plant Physiol 131:198-214.