Two-dimensional electrophoresis and mass spectrometric identification of mitochondrial proteins from an SH-SY5Y neuroblastoma cell line

Mitochondrion

Volumn 1, Issue 2, 2001, Pages 161-179

  Scheffler, N Karoline ^a,b   Miller, Scott W ^c   Carroll, Amy K ^c   Anderson, Christen ^c   Davis, Robert E ^c   Ghosh, Soumitra S ^c   Gibson, Bradford W ^a,b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b BUCK INSTITUTE FOR RESEARCH ON AGING   (United States)

^c Migenix Inc   (United States)

Author keywords

2D SDS PAGE; AD, Alzheimer's disease; CCB, colloidal Coomassie blue; DTT, dithiothreitol; ER, endoplasmic reticulum; IEF, isoelectric focusing; IPG, isoelectric pH gradient; Mass fingerprinting; Mitochondrial proteins; Neuroblastoma cell line

Indexed keywords

EID: 0000233053     PISSN: 15677249     EISSN: None     Source Type: Journal    
DOI: 10.1016/S1567-7249(01)00007-1     Document Type: Article

References (42)

1. Adessi C., Miege C., Albrieux C., Rabilloud T. Two-dimensional electrophoresis of membrane proteins: a current challenge for immobilized pH gradients. Electrophoresis. 18:1997;127-135.
2. Chen L.L., Roberts R., Friedman D.L. Expression of brain-type creatine kinase and ubiquitous mitochondrial creatine kinase in the fetal rat brain - evidence for a nuclear energy shuttle. J. Comp. Neurol. 363:1995;389-401.
3. Clauser K.R., Baker P., Burlingame A.L. Role of accurate mass measurement (±10 ppm) in protein identification strategies employing MS or MS/MS and database searching. Anal. Chem. 71:1999;2871-2882.
4. Corbett J.M., Why H.J., Wheeler C.H., Richardson P.J., Archard L.C., Yacoub M.H., Dunn M.J. Cardiac protein abnormalities in dilated cardiomyopathy detected by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis. 19:1998;2031-2042.
5. Evans G., Wheeler C.H., Corbett J.M., Dunn M.J. Construction of HSC-2DPAGE: a two-dimensional gel electrophoresis database of heart proteins. Electrophoresis. 18:1997;471-479.
6. Fiskum G., Murphy A.N., Beal M.F. Mitochondria in neurodegeneration: acute ischemia and chronic neurodegenerative diseases. J. Cereb. Blood Flow Metab. 19:1999;351-369.
7. Ghosh S.S., Miller S.E., Herrnstadt C., Dykens J.A., Murphy A.N., Davis R.E. Genetic and biochemical characterization of a cybrid cell line with a stable cytochrome c oxidase defect. Soc. Neurosci. Abstr. 25:1999a;337.
8. Ghosh S.S., Swerdlow R.H., Miller S.W., Sheeman B., Parker W.D., Davis R.E. Use of cytoplasmic hybrid cell lines for elucidating the role of mitochondrial dysfunction in Alzheimer's disease and Parkinson's disease. Ann. N. Y. Acad. Sci. 893:1999b;176-191. (A 134.9).
9. Golaz O., Hughes G.J., Frutiger S., Paquet N., Bairoch A., Pasquali C., Sanchez J.C., Tissot J.D., Appel R.D., Walzer C., Balant L., Hochstrasser D.F. Plasma and red blood cell protein maps - update 1993. Electrophoresis. 14:1993;1223-1231.
10. Gravel P., Sanchez J.C., Walzer C., Golaz O., Hochstrasser D.F., Balant L.P., Hughes G.J., Garciasevilla J., Guimon J. Human blood platelet protein map established by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis. 16:1995;1152-1159.
11. 2+. J. Biol. Chem. 274:1999;14157-14162.
12. Hartinger J., Stenius K., Hogemann D., Jahn R. 16-BAC/SDS-PAGE - a two-dimensional gel electrophoresis system suitable for the separation of integral membrane proteins. Anal. Biochem. 240:1996;126-133.
13. Hemmer W., Wallimann T. Functional aspects of creatine kinase in brain. Dev. Neurosci. 15:1993;249-260.
14. Hughes G.J., Frutiger S., Paquet N., Ravier F., Pasquali C., Sanchez J.C., James R., Tissot J.D., Bjellqvist B., Hochstrasser D.F. Plasma protein map - an update by microsequencing. Electrophoresis. 13:1992;707-714.
15. Jenner P. Oxidative stress in Parkinson's disease and other neurodegenerative disorders. Pathol. Biol. 44:1996;57-64.
16. Khan S.M., Cassarino D.S., Abramova N.N., Keeney P.M., Borland M.K., Trimmer P.A., Krebs C.T., Bennett J.C., Parks J.K., Swerdlow R.H., Parker W.D., Bennett J.P. Alzheimer's disease cybrids replicate beta-amyloid abnormalities through cell death pathways. Ann. Neurol. 48:2000;148-155.
17. Langen H., Berndt P., Roder D., Cairns N., Lubec G., Fountoulakis M. Two-dimensional map of human brain proteins. Electrophoresis. 20:1999;907-916.
18. Lopez M.F., Kristal B.S., Chernokalskaya E., Lazarev A., Shestopalov A.I., Bogdanova A., Robinson M. High-throughput profiling of the mitochondrial proteome using affinity fractionation and automation. Electrophoresis. 21:2000;3427-3440.
19. Matsui N.M., Smith D.M., Clauser K.R., Fichmann J., Andrews L.E., Sullivan C.M., Burlingame A.L., Epstein L.B. Immobilized pH gradient two-dimensional gel electrophoresis and mass spectrometric identification of cytokine-regulated proteins in ME-180 cervical carcinoma cells. Electrophoresis. 18:1997;409-417.
20. Miller S.W., Trimmer P.A., Parker W.D., Davis R.E. Creation and characterization of mitochondrial DNA-depleted cell lines with neuronal-like properties. J. Neurochem. 67:1996;1897-1907.
21. Molloy M.P., Herbert B.R., Walsh B.J., Tyler M.I., Traini M., Sanchez J.C., Hochstrasser D.F., Williams K.L., Gooley A.A. Extraction of membrane proteins by differential solubilization for separation using two-dimensional gel electrophoresis. Electrophoresis. 19:1998;837-844.
22. Oh-Ishi M., Satoh M., Maeda T. Preparative two-dimensional gel electrophoresis with agarose gels in the first dimension for high molecular mass proteins. Electrophoresis. 21:2000;1653-1669.
23. Onorato J.M., Thorps S.R., Baynes J.W. Immunohistochemical and ELISA assays for biomarkers of oxidative stress in aging and disease. Ann. N. Y. Acad. Sci. 20:1998;277-290.
24. Parker W.D., Boyson S.J., Parks J.K. Abnormalities of the electron transport chain in idiopathic Parkinsons disease. Ann. Neurol. 26:1989;719-723.
25. Parker W.D., Parks J.K. Cytochrome c oxidase in Alzheimers disease brain - purification and characterization. Neurology. 45:1995;482-486.
26. Perkins G., Renken C., Martone M.E., Young S.J., Ellisman M. Electron tomography of neuronal mitochondria: three-dimensional structure and organization of cristae and membrane contacts. J. Struct. Biol. 119:1997;260-272.
27. Rabilloud T., Valette C., Lawrence J.J. Sample application by in-gel rehydration improves the resolution of two-dimensional electrophoresis with immobilized pH gradients in the first dimension. Electrophoresis. 15:1994;1552-1558.
28. Rabilloud T., Kieffer S., Procaccio V., Louwagie M., Courchesne P.L., Patterson S.D., Martinez P., Garin J., Lunardi J. Two-dimensional electrophoresis of human placental mitochondria and protein identification by mass spectrometry: toward a human mitochondrial proteome. Electrophoresis. 19:1998;1006-1014.
29. Rickwood D., Wilson M.T., Darley-Usmar V.M. Mitochondria, A Practical Approach. 1987;IRL Press, Oxford.
30. Salim S., Filling C., Martensson E., Oppermann U.C. Lack of quinone reductase activity suggests that amyloid-beta peptide/ERAB induced lipid peroxidation is not directly related to production of reactive oxygen species by redox cycling. Toxicology. 144:2000;163-168.
31. Sanchez J.C., Hochstrasser D.F., Rabilloud T. In-gel sample rehydration of immobilized pH gradient. Methods Mol. Biol. 113:1999;221-225.
32. Sanchez J.C., Rouge V., Pisteur M., Ravier F., Tonella L., Moosmayer M., Wilkins M.R., Hochstrasser D.F. Improved and simplified in-gel sample application using reswelling of dry immobilized pH gradients. Electrophoresis. 18:1997;324-327.
33. Schulz J.B., Matthews R.T., Beal M.F. Role of nitric oxide in neurodegenerative diseases. Curr. Opin. Neurol. 8:1995;480-486.
34. Sickmann A., Dormeyer W., Wortelkamp S., Woitalla D., Kuhn W., Meyer H.E. Identification of proteins from human cerebrospinal fluid, separated by two-dimensional polyacrylamide gel electrophoresis. Electrophoresis. 21:2000;2721-2728.
35. Soltys B.J., Gupta R.S. Changes in mitochondrial shape and distribution induced by ethacrynic acid and the transient formation of a mitochondrial reticulum. J. Cell. Physiol. 159:1994;281-294.
36. Spahr C.S., Susin S.A., Bures E.J., Robinson J.H., Davis M.T., McGinley M.D., Kroemer G., Patterson S.D. Simplification of complex peptide mixtures for proteomic analysis: reversible biotinylation of cysteinyl peptides. Electrophoresis. 21:2000;1635-1650.
37. Steinberg T.H., Jones L.J., Haugland R.P., Singer V.L. SYPRO orange and SYPRO red protein gel stains - one-step fluorescent staining of denaturing gels for detection of nanogram levels of protein. Anal. Biochem. 239:1996;223-237.
38. Storrie B., Madden E.A. Isolation of subcellular organelles. Methods Enzymol. 182:1990;203-225.
39. Swerdlow R.H., Parks J.K., Cassarino D.S., Maguire D.J., Maguire R.S., Bennett J.P., Davis R.E., Parker W.D. Cybrids in Alzheimer's disease: a cellular model of the disease? Neurology. 49:1997;918-925.
40. Vestal M.L., Juhasz P., Martin S.A. Delayed extraction matrix-assisted laser desorption time-of-flight mass spectrometry. Rapid Commun. Mass Spectrom. 9:1995;1044-1050.
41. Wimmer K., Kuick R., Thoraval D., Hanash S.M. Two-dimensional separations of the genome and proteome of neuroblastoma cells. Electrophoresis. 17:1996;1741-1751.
42. Yun M., Wu W., Hood L., Harrington M. Human cerebrospinal fluid protein database - edition 1992. Electrophoresis. 13:1992;1002-1013.