High throughput protein characterization by automated reverse-phase chromatography/electrospray tandem mass spectrometry

Protein Science

Volumn 7, Issue 3, 1998, Pages 706-719

  Ducret, Axel ^a,b   Van Oostveen, Inge ^a   Eng, Jimmy K ^a   Yates III, John R ^a   Aebersold, Ruedi ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b MERCK FROSST CENTRE FOR THERAPEUTIC RESEARCH   (Canada)

Author keywords

Electrospray ionization mass spectrometry; Protein identification; Proteome; Saccharomyces cerevisiae; Sequence database; Two dimensional gel electrophoresis

Indexed keywords

FUNGAL PROTEIN;

ARTICLE; COMPUTER PROGRAM; INSTRUMENTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVERSED PHASE LIQUID CHROMATOGRAPHY; SACCHAROMYCES CEREVISIAE; TANDEM MASS SPECTROMETRY;

EID: 0031888036     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560070320     Document Type: Article

References (24)

1. Aebersold RH, Leavitt J, Saavedra RA, Hood LE, Kent SBH. 1987. Internal amino acid sequence analysis of proteins separated by one-or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose. Proc Natl Acad Sci USA 84:6970-6974.
2. Anderson NG, Anderson NL. 1996. Twenty years of two-dimensional electrophoresis: Past, present and future. Electrophoresis 17:443-453.
3. Bleasby AJ, Akrigg D, Attwood TK. 1994. OWL - A non-redundant composite protein sequence database. Nucleic Acids Res 22:3574-3577.
4. Cottrell JS. 1994. Protein identification by peptide mass fingerprinting. Peptide Res 7:115-124.
5. Davis MT, Stahl DC, Hefta SA, Lee TD. 1995. A microscale electrospray interface for on-line, capillary complex peptide mixtures. Anal Chem 67:4549-4556.
6. Ducret A, Foyn Brunn C, Bures EJ, Marhaug G, Husby G, Acbcrsold R. 1996. Characterization of human serum amyloid A protein isoforms separated by two-dimensional electrophoresis by liquid chromatography/electrospray ionization tandem mass spectrometry. Electrophoresis 17:866-876.
7. Ducret A, Gu M, Haynes PA, Yates JR III, Aebersold R. 1997. Simple design for a capillary liquid chromatography-microelectrospray-tandem mass spectrometric system for peptide mapping at the low femtomole sensitivity range. Proceedings of the ABRF'97 international symposium: Technique at the genome/proteome interface:69.
8. Eng J, McCormack AL, Yates JR III. 1994. An approach to correlate tandem mass spectral data of peptides with amino acid sequences in a protein database. J Am Mass Spectrom 5:976-989.
9. Figeys D, Ducret A, Yates JR, Aebersold R. 1996a. Protein identification by solid phase microextraction-capillary zone electrophoresis-microelectrospray-tandem mass spectrometry. Nature Biotech 14:1579-1583.
10. Figeys D, van Oostveen I, Ducret A, Aebersold R. 1996b. Protein identification by capillary zone electrophoresis/microelectrospray ionization-tandem mass spectrometry at the subfemtomole level. Anal Chem 68:1822-1828.
11. Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisal JD, Lacq L, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SB. 1996. Life with 6000 genes. Science 274:546-567.
12. Görg A, Boguth G, Obermaier C, Posch A, Weiss W. 1995. Two-dimensional polyacrylamide gel electrophoresis with immobilized pH gradients in the first dimension (IPG-Dalt): The state of the art and the controversy of vertical versus horizontal systems. Electrophoresis 16:1079-1086.
13. Haller I, Mirza UA, Chait BT. 1996. Collision induced decomposition of peptides. Choice of collision parameters. J Am Mass Spectrom 7:677-681.
14. Jazwinski M. 1990. Preparation of extracts from yeast. Methods Enzymol 182:154-174.
15. Link AJ, Hays LG, Carmack EB, Yates JR III. 1997. Identifying the major proteome components of Haemophilus influenzae type-strain NCTC 8143. Electrophoresis 18:1314-1334.
16. Mann M, Wilm M. 1994. Error-tolerant identification of peptides in sequence databases by peptide sequence tags. Anal Chem 66:4390-4399.
17. Moritz RL, Simpson RJ. 1992. Application of capillary reversed-phase high-performance liquid chromatography to high-sensitivity protein sequence analysis. J Chromatogr 599:119-130.
18. Patterson SD, Aebersold R. 1995. Mass spectrometric approaches for the identification of gel-separated protein. Electrophoresis 16:1791-1814.
19. Rabilloud T, Vuillard L, Gilly C, Lawrence JJ. 1994. Silver-staining of proteins in polyacrylamide gels: A general overview. Cell Mol Biol 40:57-75.
20. Shevchenko A, Jensen ON, Podtelejnikov AV, Sagliocco F, Wilm M, Vorm O, Mortenson P, Shevchenko A, Boucherie H, Mann M. 1996. Linking genome and proteome by mass spectrometry: Large-scale identification of yeast proteins from two dimensional gels. Proc Natl Acad Sci USA 93:14440-14445.
21. Stahl DC, Swiderek KM, Davis MT, Lee TD. 1996. Data-controlled automation of liquid chromatography/tandem mass spectrometry analysis of peptide mixtures. J Am Mass Spectrom 7:532-540.
22. Wilkins MR, Sanchez JC, Gooley AA, Appel RD, Humphrey-Smith I, Hochstrasser DF, Williams KL. 1996. Progress with proteome projects: Why all proteins expressed by a genome should be identified and how to do it. Biotech Gen Eng Reviews 13:19-50.
23. Wilm M, Shevchenko A, Houthaeve T, Breit S, Schweigerer L, Fotsis T, Mann M. 1996. Femtomole sequencing of proteins from polyacrylamide gels by nano-electrospray mass spectrometry. Nature 379:466-469.
24. Yates JR III, Eng JK, McCormack AL Schieltz D. 1995. Method to correlate tandem mass spectra of modified peptides to amino acid sequences in the protein database. Anal Chem 67:1426-1436.