Use of RT-PCR and DNA microarrays to characterize RNA recovered by non-invasive tape harvesting of normal and inflamed skin

Journal of Investigative Dermatology

Volumn 123, Issue 1, 2004, Pages 159-167

  Wong, Rita ^a   Tran, Vynga ^a   Morhenn, Vera ^b   Hung, She Pin ^c,d   Andersen, Bogi ^d   Ito, Elaine ^c,d   Hatfield, G Wesley ^c,d   Benson, Nicholas R ^a  

^a DermTech International   (United States)

^b VETERANS AFFAIRS MEDICAL CENTER   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Dermatis; DNA microarray; Epidermis; Expression profile; Inflammation; Quantitive RT PCR; Tape strip

Indexed keywords

BIOLOGICAL MARKER; DNA; RNA;

ARTICLE; BIOPSY; CELL POPULATION; CLINICAL ARTICLE; CONTROLLED STUDY; DNA MICROARRAY; EPIDERMIS CELL; FEMALE; GENE EXPRESSION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA HYBRIDIZATION; SKIN INFLAMMATION; STATISTICAL SIGNIFICANCE;

EID: 3042545218     PISSN: 0022202X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.0022-202X.2004.22729.x     Document Type: Article

References (41)

1. Aitman TJ: DNA microarrays in medical practice. Brit Med J 323:611-615, 2001
2. Allison DB, Gadbury GL: A mixture model approach for the analysis of microarray gene expression data. Comput Stat Data Analy 39:1-20, 2002
3. AppliedBiosystems (2001). User Bulletin #2: Relative quantitation of gene expression.
4. Baldi P, Hatfield GW: DNA Microarrays and Gene Expression: From Experiments to Data Analysis and Modeling. Cambridge, UK: Cambridge University Press, 2002
5. Bertucci F, Houlgatte R, Nguyen C, et al: Gene expression profiling of cancer by use of DNA arrays: How far from the clinic?. Lancet Oncol 2:674-682, 2001
6. Boelsma E, Gibbs S, Ponec M, et al: Expression of skin-derived antileukoproteinase (SKALP) in reconstructed human epidermis and its value as a marker for skin irritation. Acta Derm Venereol 78:107-113, 1998
7. Boxman IL, Hensbergen PJ, Van Der Schors RC, et al: Proteomic analysis of skin irritation reveals the induction of HSP27 by sodium lauryl sulphate in human skin. Br J Dermatol 146:777-785, 2002
8. Bustin SA: Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): Trends and problems. J Mol Endocrinol 29:23-39, 2002
9. Chung NM, Marshall CM, Leyden JJ, et al: Sodium dodecyl sulfate induces plasminogen activator inhibitor type 2 expression in epidermal keratinocytes in vivo and in vitro. J Invest Dermatol 117:647-653, 2001
10. Coquette A, Berna N, Van den Bosch A, et al: Analysis of interleukin-1alpha (IL-1alpha) and interleukin-8 (IL-8) expression and release in in vitro reconstructed human epidermis for the prediction of in vivo skin irritation and/or sensitization. Toxicol In Vitro 17:311-321, 2003
11. Corsini E, Galli CL: Cytokines and irritant contact dermatitis. Toxicol Lett 102-103: 277-282, 1998
12. Cumberbatch M, Dearman RJ, Groves RW, et al: Differential regulation of epidermal Langerhans cell migration by interleukins (IL)-1alpha and IL-1beta during irritant- and allergen-induced cutaneous immune responses. Toxicol Appl Pharmacol 182:126-135, 2002
13. Feghali CA, Wright TM: Cytokines in acute and chronic inflammation. Front Biosci 2:12-d26, 1997
14. Flier J, Boorsma DM, Bruynzeel DP, et al: The CXCR3 activating chemokines IP-10, Mig, and IP-9 are expressed in allergic but not in irritant patch test reactions. J Invest Dermatol 113:574-578, 1999
15. Freedberg IM, Tomic-Canic M, Komine M, et al: Keratins and the keratinocyte activation cycle. J Invest Dermatol 116:633-640, 2001
16. Galiegue S, Casellas P: Exploitation of expression profiles: examples in oncology. J Soc Biol 196:313-315, 2002
17. Gibson UE, Heid CA, Williams PM, et al: A novel method for real time quantitative RT-PCR. Genome Res 6:995-1001, 1996
18. Grangsjo A, Leijon-Kuligowski A, Torma H, et al: Different pathways in irritant contact eczema? Early differences in the epidermal elemental content and expression of cytokines after application of 2 different irritants. Contact Dermatitis 35:355-360, 1996
19. Heid CA, Stevens J, Livak KJ, et al: Real time quantitative PCR. Genome Res 6:986-994, 1996
20. Hoefakker S, Caubo M, van't Erve EH, et al: In vivo cytokine profiles in allergic and irritant contact dermatitis. Contact Dermatitis 33:258-266, 1995
21. Hoffrage U, Lindsey S, Hertwig R, et al: Communicating statistical information. Science 290:2261-2262, 2000
22. Howie SE, Aldridge RD, McVittie E, et al: Epidermal keratinocyte production of interferon-gamma immunoreactive protein and mRNA is an early event in allergic contact dermatitis. J Invest Dermatol 106:1218-1223, 1996
23. Hung S-P, Baldi P, Hatfield GW, et al: Global gene expression profiling in Escherichia coli K12: The effects of leucine-responsive regulatory protein. J Biol Chem 277:40309-40323, 2002
24. Komine M, Rao LS, Freedberg IM, et al: Interleukin-1 induces transcription of keratin K6 in human epidermal keratinocytes. J Invest Dermatol 116:330-338, 2001
25. Lacroix M, Zammatteo N, Remade J, et al: A tow-density DNA microarray for analysis of markers in breast cancer. Int J Biol Markers 17:5-23, 2002
26. Li C, Wong WH: Model-based analysis of oligonucleotide arrays: Expression index computation and outlier detection. Proc Natl Acad Sci USA 98:31-36, 2001
27. Long AD, Mangalam HJ, Chan BY, et al: Improved statistical inference from DNA microarray data using analysis of variance and a Bayesian Global gene expression profiling in Escherichia coli K12. The effects of integration host factor. J Biol Chem 276:19937-19944, 2001
28. Morhenn VB, Chang EY, Rheins LA: A noninvasive method for quantifying and distinguishing inflammatory skin reactions. J Am Acad Dermatol 41:687-692, 1999
29. Muller-Decker K, Furstenberger G, Marks F: Keratinocyte-derived proinflammatory key mediators and cell viability as in vitro parameters of irritancy: A possible alternative to the Draize skin irritation test. Toxicol Appl Pharmacol 127:99-108, 1994
30. Muller-Decker K, Heinzelmann T, Furstenberger G, et al: Arachidonic acid metabolism in primary irritant dermatitis produced by patch testing of human skin with surfactants. Toxicol Appl Pharmacol 153:59-67, 1998
31. Paludan K, Thestrup-Pedersen K: Use of the polymerase chain reaction in quantification of interleukin 8 mRNA in minute epidermal samples. J Invest Dermatol 99:830-835, 1992
32. Perkins MA, Cardin CW, Osterhues MA, et al: A non-invasive tape absorption method for recovery of inflammatory mediators to differentiate normal from compromised scalp conditions. Skin Res Technol 8:187-193, 2002
33. Perkins MA, Osterhues MA, Farage MA, et al: A noninvasive method to assess skin irritation and compromised skin conditions using simple tape adsorption of molecular markers of inflammation. Skin Res Technol 7:227-237, 2001
34. Ryan CA, Gerberick GF: Cytokine mRNA expression in human epidermis after patch treatment with rhus and sodium lauryl sulfate. Am J Contact Dermat 10:127-135, 1999
35. Satagopan JM, Panageas KS: A statistical perspective on gene expression data analysis. Stat Med 22:481-499, 2003
36. Suzuki T, Higgins PJ, Crawford DR, et al: Control selection for RNA quantitation. Biotechniques 29:332-337, 2000
37. Tomic-Canic M, Komine M, Freedberg IM, et al: Epidermal signal transduction and transcription factor activation in activated keratinocytes. J Dermatol Sci 17:167-181, 1998
38. Tricarico C, Pinzani P, Bianchi S, et al: Quantitative real-time reverse transcription polymerase chain reaction: Normalization to rRNA or single house-keeping genes is inappropriate for human tissue biopsies. Anal Biochem 309:293-300, 2002
39. Ulfgren AK, Klareskog L, Lindberg M, et al: An immunohistochemical analysis of cytokine expression in allergic and irritant contact dermatitis. Acta Derm Venereol 80:167-170, 2000
40. van Ruissen F, Le M, Carroll JM, et al: Differential effects of detergents on keratinocyte gene expression. J Invest Dermatol 110:358-363, 1998
41. Whipple ME, Kuo WP: DNA microarrays in otolaryngology-head and neck surgery. Otolaryngol Head Neck Surg 127:196-204, 2002