Probing the substrate binding site of Candida tenuis xylose reductase (AKR2B5) with site-directed mutagenesis

Biochemical Journal

Volumn 393, Issue 1, 2006, Pages 51-58

  Kratzer, Regina ^a   Leitgeb, Stefan ^a,b   Wilson, David K ^b   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Aldo keto reductase (AKR); Candida tenais xylose reductase; Hydrogen bonding; Ketone reduction; Structure activity correlation; Substrate selectivity

Indexed keywords

ALDEHYDES; CATALYSIS; HYDROGEN BONDS; KETONES; MUTAGENESIS; MUTAGENS; OXIDATION; REDUCTION; SUBSTRATES; X RAY CRYSTALLOGRAPHY; XYLOSE;

KETONE REDUCTION; STRUCTURE-ACTIVITY CORRELATION; SUBSTRATE SELECTIVITY;

PROTEINS;

ALANINE; ASPARAGINE; ASPARTIC ACID; DEOXYGALACTOSE; GALACTOSE; GLUCOSE; GLYCERALDEHYDE; HOLOENZYME; HYDROGEN; OXIDOREDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE; TRYPTOPHAN; UNCLASSIFIED DRUG; XYLITOL; XYLOSE; XYLOSE REDUCTASE;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; CANDIDA; CANDIDA TENUIS; CATALYSIS; ENERGY TRANSFER; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; HYDROGEN BOND; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN FAMILY; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS; STRUCTURE ACTIVITY RELATION; SUBSTITUTION REACTION; WILD TYPE; X RAY CRYSTALLOGRAPHY;

ALDEHYDE REDUCTASE; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BINDING SITES; CANDIDA; CONSERVED SEQUENCE; CRYSTALLOGRAPHY, X-RAY; KINETICS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

CANDIDA; CANDIDA TENUIS;

EID: 30044432930     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050831     Document Type: Article

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