Electrostatic stabilization in a pre-organized polar active site: The catalytic role of Lys-80 in Candida tenuis xylose reductase (AKR2B5) probed by site-directed mutagenesis and functional complementation studies

Biochemical Journal

Volumn 389, Issue 2, 2005, Pages 507-515

  Kratzer, Regina ^a   Nidetzky, Bernd ^a  

^a GRAZ UNIVERSITY OF TECHNOLOGY   (Austria)

Author keywords

Active site; Aldo keto reductase; Candida tenuis; Catalytic mechanism; Chemical rescue; Xylose reductase

Indexed keywords

AMINES; CARBONYLATION; CATALYSIS; HYDRIDES; HYDROPHOBICITY; KINETIC THEORY; PH EFFECTS; PROTEINS;

ALDO-KETO REDUCTASE PROTEINS; AMINE GROUPS; CARBONYL GROUP REDUCTIONS; XYLOSE REDUCTASE;

XYLOSE;

ALANINE; ASPARAGINE; ASPARTIC ACID; FUNGAL PROTEIN; LYSINE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; OXIDOREDUCTASE; PROTEIN XYLOSE REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TYROSINE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING AFFINITY; BINDING KINETICS; CANDIDA; CANDIDA TENUIS; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME KINETICS; ENZYME SPECIFICITY; HYDROPHOBICITY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN STABILITY; PROTON TRANSPORT; SITE DIRECTED MUTAGENESIS;

ALDEHYDE REDUCTASE; BINDING SITES; CANDIDA; CATALYSIS; ELECTROSTATICS; GENE EXPRESSION REGULATION, FUNGAL; GENETIC COMPLEMENTATION TEST; HYDROGEN-ION CONCENTRATION; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTATION; NADP; OXIDATION-REDUCTION; PARGYLINE; PROPYLAMINES; PROTEIN BINDING; SUBSTRATE SPECIFICITY; XYLITOL; XYLOSE;

CANDIDA TENUIS;

EID: 22644437239     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20050167     Document Type: Article

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