Crystal structure of anticoagulant thrombin variant E217K provides insights into thrombin allostery

Journal of Biological Chemistry

Volumn 279, Issue 25, 2004, Pages 26387-26394

  Carter, Wendy J ^a   Myles, Timothy ^b   Gibbs, Craig S ^c   Leung, Lawrence L ^b   Huntington, James A ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c GILEAD SCIENCES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BLOOD; CASCADE CONNECTIONS; CATALYSIS; HYDROGEN BONDS; PROTEINS;

ANTICOAGULANT; BLOOD CLOTTING; THROMBIN;

CRYSTAL STRUCTURE;

ANTICOAGULANT AGENT; ANTICOAGULANT PROTEIN; PROTEIN C; SODIUM ION; THROMBIN; THROMBIN E217K; THROMBOMODULIN; UNCLASSIFIED DRUG;

ALLOSTERISM; ARTICLE; BINDING SITE; BLOOD CLOTTING; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME INACTIVATION; ENZYME STRUCTURE; ENZYME SUBSTRATE; FIBRIN CLOT; FIBRINOGEN METABOLISM; GEOMETRY; HYDROGEN BOND; MUTAGENESIS; PRIORITY JOURNAL;

ALLOSTERIC SITE; BINDING SITES; CATALYSIS; CRYSTALLOGRAPHY, X-RAY; HUMANS; HYDROGEN BONDING; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PROTEIN BINDING; PROTEIN CONFORMATION; SODIUM; THROMBIN;

EID: 2942703945     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M402364200     Document Type: Article

References (37)

1. Stubbs, M. T. & Bode, W. (1993) Thromb. Res. 69, 1-58
2. Stubbs, M. T. & Bode, W. (1995) Trends Biochem. Sci. 20, 23-28
3. Huntington, J. A. & Baglin, T. P. (2003) Trends Pharmacol. Sci. 24, 589-595
4. Huntington, J. A. (2003) J. Thromb. Haemostasis 1, 1535-1549
5. Esmon, C. T. (1987) Science 235, 1348-1352
6. Esmon, C. T. (2003) Chest 124, 26S-32S
7. Guillin, M. C., Bezeaud, A., Bouton, M. C. & Jandrot-Perrus, M. (1995) Thromb. Haemostasis 74, 129-133
8. Di Cera, E., Dang, Q. D. & Ayala, Y. M. (1997) Cell Mol. Life Sci. 53, 701-730
9. Verhamme, I. M., Olson, S. T., Tollefsen, D. M. & Bock, P. E. (2002) J. Biol. Chem. 277, 6788-6798
10. Huntington, J. A. & Esmon, C. T. (2003) Structure 11, 469-479
11. Wells, C. M. & Di Cera, E. (1992) Biochemistry 31, 11721-11730
12. Zhang, E. & Tulinsky, A. (1997) Biophys. Chem. 63, 185-200
13. Di Cera, E., Guinto, E. R., Vindigni, A., Dang, Q. D., Ayala, Y. M., Wuyi, M. & Tulinsky, A. (1995) J. Biol. Chem. 270, 22089-22092
14. Dang, O. D., Vindigni, A. & Di Cera, E. (1995) Proc. Natl. Acad. Sci. U. S. A. 92, 5977-5981
15. Parry, M. A., Stone, S. R., Hofsteenge, J. & Jackman, M. P. (1993) Biochem. J. 290, 665-670
16. Lai, M. T., Di Cera, E. & Shafer, J. A. (1997) J. Biol. Chem. 272, 30275-30282
17. Pineda, A. O., Savvides, S., Waksman, G. & Di Cera, E. (2002) J. Biol. Chem. 277, 40177-40180
18. Hall, S. W., Gibbs, C. S. & Leung, L. L. (1997) Cell Mol. Life Sci. 53, 731-736
19. Gibbs, C. S., Coutre, S. E., Tsiang, M., Li, W. X., Jain, A. K., Dunn, K. E., Law, V. S., Mao, C. T., Matsumura, S. Y. & Mejza, S. J. (1995) Nature 378, 413-416
20. Tsiang, M., Paborsky, L. R., Li, W. X., Jain, A. K., Mao, C. T., Dunn, K. E., Lee, D. W., Matsumura, S. Y., Matteucci, M. D., Coutre, S. E., Leung, L. L. & Gibbs, C. S. (1996) Biochemistry 35, 16449-16457
21. Cantwell, A. M. & Di Cera, E. (2000) J. Biol. Chem. 275, 39827-39830
22. Gruber, A., Cantwell, A. M., Di Cera, E. & Hanson, S. R. (2002) J. Biol. Chem. 277, 27581-27584
23. Collaborative Computing Project Number 4 (1994) Acta Crystallogr. Sect. D Biol. Crystallogr. 50, 760
24. Vagin, A. & Teplyakov, A. (2000) Acta Crystallogr. Sect. D Biol. Crystallogr. 56 (Pt. 12), 1622-1624
25. Brunger, A. T., Adams, P. D., Clore, G. M., Delano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T. & Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921
26. Esnouf, R. M. (1997) J. Mol. Graph. Model. 15, 132
27. Merritt, E. A. & Murphy, M. E. P. (1994) Acta Crystallogr. Sect. D Biol. Crystallogr. 50, 869-873
28. Boissel, J. P., Le Bonniec, B., Rabiet, M. J., Labie, D. & Elion, J. (1984) J. Biol. Chem. 259, 5691-5697
29. Vijayalakshmi, J., Padmanabhan, K. P., Mann, K. G. & Tulinsky, A. (1994) Protein Sci. 3, 2254-2271
30. Bode, W., Mayr, I., Baumann, U., Huber, R., Stone, S. R. & Hofsteenge, J. (1989) EMBO J. 8, 3467-3475
31. Edwards, P. D., Mauger, R. C., Cottrell, K. M., Morris, F. X., Pine, K. K., Sylvester, M. A., Scott, C. W. & Furlong, S. T. (2000) Bioorg. Med. Chem. Lett. 10, 2291-2294
32. Schechter, I. & Berger, A. (1967) Biochem. Biophys. Res. Commun. 27, 157-162
33. Baglin, T. P., Carrell, R. W., Church, F. C., Esmon, C. T. & Huntington, J. A. (2002) Proc. Natl. Acad. Sci. U. S. A. 99, 11079-11084
34. Stubbs, M. T., Oschkinat, H., Mayr, I., Huber, R., Angliker, H., Stone, S. R. & Bode, W. (1992) Eur. J. Biochem. 206, 187-195
35. Dang, Q. D., Guinto, E. R. & Di Cera, E. (1997) Nat. Biotechnol. 15, 146-149
36. Guinto, E. R. & Di Cera, E. (1997) Biophys. Chem. 64, 103-109
37. Fuentes-Prior, P., Iwanaga, Y., Huber, R., Pagila, R., Rumennik, G., Seto, M., Morser, J., Light, D. R. & Bode, W. (2000) Nature 404, 518-525