Kinetic isotope effects in Ras-catalyzed GTP hydrolysis: Evidence for a loose transition state

Proceedings of the National Academy of Sciences of the United States of America

Volumn 101, Issue 24, 2004, Pages 8858-8863

  Du, Xinlin ^a   Black, Gavin E ^b   Lecchi, Paolo ^b   Abramson, Fred P ^b   Sprang, Stephen R ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

^b GEORGE WASHINGTON UNIVERSITY MEDICAL CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CARBON 13; GUANOSINE TRIPHOSPHATE; ISOTOPE; OXYGEN 18; RAS PROTEIN;

ARTICLE; CATALYSIS; CHEMICAL REACTION; DISSOCIATION; GENE FUNCTION; ISOTOPE LABELING; MASS SPECTROMETER; ONCOGENE RAS; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; REACTION ANALYSIS;

CARBON ISOTOPES; CATALYSIS; GUANOSINE TRIPHOSPHATE; HYDROLYSIS; KINETICS; MODELS, CHEMICAL; MODELS, MOLECULAR; OXYGEN ISOTOPES; RAS PROTEINS; RECOMBINANT PROTEINS;

EID: 2942643930     PISSN: 00278424     EISSN: None     Source Type: Journal    
DOI: 10.1073/pnas.0401675101     Document Type: Article

References (54)

1. Bourne, H. R., Sanders, D. A. & McCormick, F. (1990) Nature 348, 125-132.
2. Shields, J. M., Pruitt, K., McFall, A., Shaub, A. & Der, C. J. (2000) Trends Cell Biol. 10, 147-154.
3. Feig, L. A. & Buchsbaum, R. J. (2002) Curr. Biol. 12, R259-R261.
4. Cox, A. D. & Der, C. J. (2003) Oncogene 22, 8999-9006.
5. Sprang, S. R. (1997) Annu. Rev. Biochem. 66, 639-678.
6. Ahmadian, M. R., Stege, P., Scheffzek, K. & Wittinghofer, A. (1997) Nat. Struct. Biol. 4, 686-689.
7. Bos, J. L. (1988) Mutat. Res. 195, 255-271.
8. Feuerstein, J., Goody, R. S. & Webb, M. R. (1989) J. Biol. Chem. 264, 6188-6190.
9. Maegley, K. A., Admiraal, S. J. & Herschlag, D. (1996) Proc. Natl. Acad. Sci. USA 93, 8160-8166.
10. Admiraal, S. J. & Herschlag, D. (1995) Chem. Biol. 2, 729-739.
11. Admiraal, S. J. & Herschlag, D. (2000) J. Am. Chem. Soc. 122, 2145-2148.
12. Schweins, T., Geyer, M., Scheffzek, K., Warshel, A., Kalbitzer, H. R. & Wittinghofer, A. (1995) Nat. Struct, Biol. 2, 36-44.
13. Schweins, T., Geyer, M., Kalbitzer, H. R., Wittinghofer, A. & Warshel, A. (1996) Biochemistry 35, 14225-14231.
14. Schweins, T. & Warshel, A. (1996) Biochemistry 35, 14232-14243.
15. Florian, J. & Warshel, A. (1997) J. Am. Chem. Soc. 119, 5473-5474.
16. Aqvist, J., Kolmodin, K., Florian, J. & Warshel, A. (1999) Chem. Biol. 6, R71-R80.
17. Benkovic, S. J. & Schray, K. J. (1978) in Transition States of Biochemical Processes, eds. Grandour, R. D. & Schowen, R. (Plenum, New York), pp. 493-527.
18. Thatcher, G. & Kluger, R. (1989) in Advances in Physical Organic Chemistry, ed. Bethell, D. (Academic, New York), Vol. 25, pp. 99-265.
19. Hengge, A. C. (2001) FEBS Lett. 501, 99-102.
20. Florian, J., Aqvist, J. & Warshel, A. (1998) J. Am. Chem. Soc. 120, 11524-11525.
21. Pai, E. F., Krengel, U., Petsko, G. A., Goody, R. S., Kabsch, W. & Wittinghofer, A. (1990) EMBO J. 9, 2351-2359.
22. Milburn, M. V., Tong, L., deVos, A. M., Brünger, A., Yamaizumi, Z., Nishimura, S. & Kim, S. H. (1990) Science 247, 939-945.
23. Scheffzek, K., Ahmadian, M. R., Kabsch, W., Wiesmüller, L., Lautwein, A., Schmitz, F. & Wittinghofer, A. (1997) Science 277, 333-338.
24. Glennon, T. M., Villa, J. & Warshel, A. (2000) Biochemistry 39, 9641-9651.
25. Neal, S. E., Eccleston, J. F. & Webb, M. (1990) Proc. Natl. Acad. Sci. USA 87, 3562-3565.
26. Moore, K. J. M., Webb, M. R. & Eccleston, J. F. (1993) Biochemistry 32, 7451-7459.
27. Rensland, H., Lautwein, A., Wittinghofer, A. & Goody, R. S. (1991) Biochemistry 30, 11181-11185.
28. Cleland, W. W. & Hengge, A. C. (1995) FASEB J. 9, 1585-1594.
29. Hengge, A. C. (2002) Acc. Chem. Res. 35, 105-112.
30. Northrop, D. B. (1975) Biochemistry 14, 2644-2650.
31. O'Leary, M. H. & Marlier, J. F. (1979) J. Am. Chem. Soc. 101, 3300-3306.
32. Cleland, W. W. (1995) Methods Enzymol. 249, 341-373.
33. Abramson, F. P., Black, G. E. & Lecchi, P. (2001) J. Chromatogr. A 913, 269-273.
34. Chen, P. & Abramson, F. P. (1998) Anal. Chem. 70, 1664-1669.
35. Teffera, Y., Kusmierz, J. J. & Abramson, F. P. (1996) Anal. Chem. 68, 1888-1894.
36. Lowe, G. & Sproat, B. (1978) J. Chem. Soc. Perkin Trans. 1, 1622-1630.
37. Du, X., Frei, H. & Kim, S. H. (2000) J. Biol. Chem. 275, 8492-8500.
38. Webb, M. (1980) Biochemistry 19, 4744-4748.
39. Lenzen, C., Cool, R. H., Prinz, H., Kuhlmann, J. & Wittinghofer, A. (1998) Biochemistry 37, 7420-7430.
40. Hermes, J. D., Roeske, C. A., O'Leary, M. H. & Cleland, W. W. (1982) Biochemistry 21, 5106-5114.
41. Grissom, C. B. & Cleland, W. W. (1987) Biochemistry 24, 944-948.
42. Hengge, A. C., Sowa, G. A., Wu, L. & Zhang, Z. Y. (1995) Biochemistry 34, 13982-13987.
43. Hengge, A. C., Denu, J. M. & Dixon, J. E. (1996) Biochemistry 35, 7084-7092.
44. Hoff, R. H., Hengge, A. C., Wu, L., Keng, Y. & Zhang, Z. (2000) Biochemistry 39, 46-54.
45. Hengge, A. C., Zhao, Y., Wu, L. & Zhang, Z. Y. (1997) Biochemistry 36, 7928-7936.
46. Hengge, A. C., Edens, W. A. & Elsing, H. (1994) J. Am. Chem. Soc. 116, 5045-5049.
47. Horn, H. & Ahlrichs, R. (1990) J. Am. Chem. Soc. 112, 2121-2124.
48. Rajca, A., Rice, J. E., Streitweiser, A. & Schaefer, H. F. (1987) J. Am. Chem. Soc. 109, 4189-4192.
49. Knight, W. B., Weiss, P. M. & Cleland, W. W. (1998) J. Am. Chem. Soc. 108, 2759-2761.
50. Caldwell, S. R., Raushel, F. M., Weiss, P. M. & Cleland, W. W. (1991) Biochemistry 30, 7444-7450.
51. Dever, T. E., Glynias, M. J. & Merrick, W. C. (1987) Proc. Natl. Acad. Sci. USA 84, 1814-1818.
52. Traut, T. W. (1994) Eur. J. Biochem. 222, 9-19.
53. Gerwert, K. & Cepus, V. (2001) Biochemistry 40, 3037-3046.
54. Wang, J. H., Xiao, D. G., Deng, H., Webb, M. R. & Callender, R. (1998) Biochemistry 37, 11106-11116.