A 96-well automated method to study inhibitors of human sodium-dependent D-glucose transport

Molecular and Cellular Biochemistry

Volumn 280, Issue 1-2, 2005, Pages 91-98

  Castaneda, Francisco ^a   Kinne, Rolf K H ^a  

^a MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

Author keywords

14C methyl D glucopyranoside; Kinetic studies; Sodium dependent D glucose transporter; Transport inhibitor

Indexed keywords

ALPHA METHYLGLUCOSIDE; CARBON 14; CARRIER PROTEIN; HYGROMYCIN; PROTEIN INHIBITOR; RADIOACTIVE MATERIAL; SODIUM GLUCOSE COTRANSPORTER; SODIUM GLUCOSE COTRANSPORTER 1; SODIUM GLUCOSE COTRANSPORTER 2; UNCLASSIFIED DRUG;

ANALYZER; ANIMAL CELL; ARTICLE; AUTOMATION; CELL CLONE; CHO CELL; CONTROLLED STUDY; CULTURE MEDIUM; GENE EXPRESSION; GENETIC TRANSFECTION; GLUCOSE TRANSPORT; HIGH THROUGHPUT SCREENING; IMMUNOCYTOCHEMISTRY; MEMBRANE TRANSPORT; NONHUMAN; PLASMID; PROCESS DEVELOPMENT; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; TRANSPORT KINETICS; WESTERN BLOTTING;

ANIMALS; AUTOMATION; BIOLOGICAL TRANSPORT; BLOTTING, WESTERN; CELLS, CULTURED; CHO CELLS; CRICETINAE; DRUG EVALUATION, PRECLINICAL; GENE EXPRESSION; GLUCOSE; HUMANS; INHIBITORY CONCENTRATION 50; PHLORHIZIN; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; SODIUM-GLUCOSE TRANSPORT PROTEINS; SODIUM-GLUCOSE TRANSPORTER 1; SODIUM-GLUCOSE TRANSPORTER 2; TRANSFECTION;

ANIMALIA; CRICETULUS GRISEUS;

EID: 29244475087     PISSN: 03008177     EISSN: 15734919     Source Type: Journal    
DOI: 10.1007/s11010-005-8235-y     Document Type: Article

References (30)

1. Wood IS, Trayhurn P: Glucose traEsporters (GLUT and SGLT): expanded families of sugar transport proteins. Br J Nutr 89: 3-9, 2003 12568659
2. Wright EM, Loo DD, Panayotova-Heiermann M, Lostao MP, Hirayama BH, Mackenzie B, Boorer K, Zampighi G: 'Active' sugar transport in eukaryotes. J Exp Biol 196: 197-212, 1994 7823022
3. +/ glucose cotransporters. J Exp Biol 200: 287-293, 1997 9050236
4. Wright EM: Renal Na(+)-glucose cotransporters. Am J Physiol 280: F10-F18, 2001 1:CAS:528:DC%2BD3MXjtVyku7g%3D
5. Diez-Sampedro A, Wright EM, Hirayama BA: Residue 457 controls sugar binding and transport in the Na(+)/glucose cotransporter. J Biol Chem 276: 49188-49194, 2001 10.1074/jbc.M108286200
6. + cotransport system in the intestinal brush-border membrane by using the specific substrate, methyl alpha-D-glucopyranoside. Biochim Biophys Acta 904: 71-80, 1987 3663668
7. +/glucose cotransporter: I. Voltage-clamp studies. J Memb Biol 125: 49-62, 1992 1:CAS:528:DyaK38XhsV2ju7o%3D
8. +-dependent transport system of intestinal epithelium. Am J Physiol 241: C227-C232, 1981 7304734
9. Sakhrani LM, Badie-Dezfooly B, Trizna W, Mikhail N, Lowe AG, Taub M, Fine LG: Transport and metabolism of glucose by renal proximal tubular cells in primary culture. Am J Physiol 246: F757-764, 1984 6742127
10. Amos AF, McCarty DJ, Zimmet P: The rising global burden of diabetes and its complications: Estimates and projections to the year 2010. Diabetic Med 14(suppl 5): S1-S85, 1997 9450510
11. King H, Aubert RE, Herman WH: Global burden of diabetes, 1995-2025: prevalence, numerical estimates, and projections. Diabetes Care 21: 1414-1431, 1998 9727886
12. Van Gaal LF, De Leeuw IH: Rationale and options for combination therapy in the treatment of Type 2 diabetes. Diabetologia 46(suppl 1): M44-M50, 2003 12652358
13. O'Gorman S, Fox DT, Wahl GM: Recombinase-mediated gene activation and site-specific integration in mammalian cells. Science 251: 1351-1355, 1991 1900642
14. Sauer B: Site-specific Decombination: developments and applications. Curr Opin Biotechnol 5: 521-527, 1994 10.1016/0958-1669(94)90068-X
15. Pfaffl MW: A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res 29: E45, 2001 10.1093/nar/29.9.e45
16. Stoscheck CM: Quantitation of protein. Methods Enzymol 182: 50-68, 1990 2314256
17. +/D-glucose cotransporter (SGLT1) in a stably transfected Chinese hamster ovary cell line. Biochim Biophys Acta 1373: 309-320, 1998 9733990
18. Burlingham BT, Widlanski TS: An intuitive look at the relationship of Ki and IC50: A more general use for the Dixon plot. J Chem Educ 80: 214-218, 2003 1:CAS:528:DC%2BD3sXmtFOitA%3D%3D
19. +-glucose cotransporters (SGLT-1). Am J Physiol 270: G919-G926, 1996
20. Lin J-T, Kormanec J, Homerová D, Kinne RKH: Probing transmembrane topology of the high-affinity sodium/glucose cotransporter (SGLT1) with histidine-tagged mutants. J Memb Biol 170: 243-252, 1999 1:CAS:528:DyaK1MXlt1Gksbo%3D
21. Walgren RA, Lin JT, Kinne RKH, Walle T: Cellular uptake of dietary flavonoid quercetin 4′-beta-glucoside by sodium-dependent glucose transporter SGLT1. J Pharmacol Exp Ther 294: 837-843, 2000 10945831
22. Tsujihara K, Hongu M, Saito K, Inamasu M, Arakawa K, Oku A, Matsumoto M: Na(+)-glucose cotransporter inhibitors as antidiabetics: I. Synthesis and pharmacological properties of 4′-dehydroxyphlorizin derivatives based on a new concept. Chem Pharm Bull 44: 1174-1180, 1996 8814948
23. Hongu M, Tanaka T, Funami N, Saito K, Arakawa K, Matsumoto M, Tsujihara K: Na(+)-glucose cotransporter inhibitors as antidiabetic agents: II. Synthesis and structure-activity relationships of 4′-dehydroxyphlorizin derivatives. Chem Pharm Bull 46: 22-33, 1998 9468634
24. Hongu M, Funami N, Takahashi Y, Saito K, Arakawa K, Matsumoto M, Yamakita H, Tsujihara K: Na(+)-glucose cotransporter inhibitors as antidiabetic agents: III. Synthesis and pharmacological properties of 4′-dehydroxyphlorizin derivatives modified at the OH groups of the glucose moiety. Chem Pharm Bull 46: 1545-1555, 1998 9810692
25. Tsujihara K, Hongu M, Saito K, Kawanishi H, Kuriyama K, Matsumoto M, Oku A, Ueta K, Tsuda M, Saito A: Na(+)-glucose cotransporter (SGLT) inhibitors as antidiabetic agents: 4. Synthesis and pharmacological properties of 4′-dehydroxyphlorizin derivatives substituted on the B ring. J Med Chem 42: 5311-5324, 1999 10.1021/jm990175n
26. +-glucose cotransporters, may provide a novel approach to treating diabetes. Diabetes 48: 1794-1800, 1999 10480610
27. Oku A, Ueta K, Nawano M, Arakawa K, Kano-Ishihara T, Matsumoto M, Saito A, Tsujihara K, Anai M, Asano T: Antidiabetic effect of T-1095, an inhibitor of Na(+)-glucose cotransporter, in neonatally streptozotocin-treated rats. Eur J Pharmacol 391: 183-192, 2000 10.1016/ S0014-2999(00)00016-9
28. +-glucose cotransporters in streptozotocin-induced diabetic rats. Biol Pharm Bull 23: 1434-1437, 2000 11145172
29. Arakawa K, Ishihara T, Oku A, Nawano M, Ueta K, Kitamura K, Matsumoto M, Saito A: Improved diabetic syndrome in C57BL/KsJ-db/db mice by oral administration of the Na(+)-glucose cotransporter inhibitor T-1095. Br J Pharmacol 132: 578-586, 2001 10.1038/sj.bjp.0703829
30. Wagman AS, Nuss JM: Current therapies and emerging targets for the treatment of diabetes. Curr Pharm Des 7: 417-450, 2001 10.2174/ 1381612013397915