RNA interference against Hec1 inhibits tumor growth in vivo

Gene Therapy

Volumn 13, Issue 1, 2006, Pages 1-7

  Gurzov, E N ^a   Izquierdo, M ^a  

^a UNIVERSIDAD AUTÓNOMA DE MADRID   (Spain)

Author keywords

Glioma; Hec 1; HeLa cells; RNA interference

Indexed keywords

ADENOVIRUS VECTOR; CELL PROTEIN; HIGHLY EXPRESSED IN CANCER 1 PROTEIN; RETROVIRUS VECTOR; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG;

ADENOCARCINOMA; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ANTINEOPLASTIC ACTIVITY; ARTICLE; CANCER INHIBITION; CANCER SIZE; CONTROLLED STUDY; GENE EXPRESSION; GLIOBLASTOMA; HELA CELL; HUMAN; HUMAN CELL; MITOSIS; MITOSIS INHIBITION; NONHUMAN; NUCLEOTIDE SEQUENCE; NUDE MOUSE; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN FUNCTION; PROTEIN SYNTHESIS INHIBITION; PROTEIN TARGETING; RNA INTERFERENCE; TUMOR CELL LINE; VIRAL GENE DELIVERY SYSTEM;

ADENOCARCINOMA; ADENOVIRIDAE; ANIMALS; BRAIN NEOPLASMS; CHROMOSOME SEGREGATION; FEMALE; FLOW CYTOMETRY; GENE THERAPY; GENETIC VECTORS; GLIOBLASTOMA; HELA CELLS; HUMANS; MICE; MICE, NUDE; MICROSCOPY, FLUORESCENCE; MITOSIS; NEOPLASM TRANSPLANTATION; NEOPLASMS; NUCLEAR PROTEINS; RETROVIRIDAE; RNA INTERFERENCE; RNA, SMALL INTERFERING; UTERINE CERVICAL NEOPLASMS;

ADENOVIRIDAE; ANIMALIA; MUS MUSCULUS;

EID: 29244473831     PISSN: 09697128     EISSN: 14765462     Source Type: Journal    
DOI: 10.1038/sj.gt.3302595     Document Type: Article

References (32)

1. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNAi in Caenorhabditis elegans. Nature 1998; 391: 806-811.
2. Cerutti H. RNA interference: Traveling in the cell and gaining functions? TRENDS Genet 2003; 19: 39-46.
3. Hannon GJ, Rossi JJ. Unlocking the potential of the human genome with RNA interference. Nature 2004; 431: 371-378.
4. Izquierdo M. Short interfering RNAs as a tool for cancer gene therapy. Cancer Gene Ther 2005; 12: 217-227.
5. Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tuschl T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature 2001; 411: 494-498.
6. Hannon GJ. RNA interference. Nature 2002; 418: 244-251.
7. McManus MT, Sharp PA. Gene silencing in mammals by small interfering RNAs. Nat Rev Genet 2002; 3: 737-747.
8. Brummelkamp TR, Bernards R, Agami RA. System for stable expression of short interfering RNAs in mammalian cells. Science 2002; 296: 550-553.
9. Brummelkamp TR, Bernards R, Agami R. Stable suppression of tumorigenicity by virus-mediated RNA interference. Cancer Cell 2002; 2: 243-247.
10. Devroe E, Silver PA. Retrovirus-delivered shRNA. BMC Biotechnol 2002; 2: 15-20.
11. Cleveland DW, Mao Y, Sullivan KF. Centromeres and kinetochores: From epigenetics to mitotic checkpoint signaling. Cell 2003; 112: 407-421.
12. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M. A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Nat Cell Biol 2004; 6: 1135-1141.
13. Steensgaard P, Garre M, Muradore I, Transidico P, Nigg EA, Kitagawa K et al. Sgt1 is required for human kinetochore assembly. EMBO Rep 2004; 5: 626-631.
14. Wigge PA, Kilmartin JJ. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J Cell Biol 2001; 152: 349-360.
15. Martin-Lluesma S, Stucke VM, Nigg EA. Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2. Science 2002; 297: 2267-2270.
16. DeLuca JG, Moree B, Hickey JM, Kilmartin JV, Salmon ED. hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells. J Cell Biol 2002; 159: 549-554.
17. DeLuca JG, Howell BJ, Canman JC, Hickey JM, Fang G, Salmon ED. Nuf2 and Hec1 are required for retention of the checkpoint proteins Mad1 and Mad2 to kinetochores. Curr Biol 2003; 13: 2103-2109.
18. Chen Y, Riley DJ, Zheng L, Chen PL, Lee WH. Phosphorilation of the mitotic regulator protein Hec1 by Nek2 kinase is essential for faithful chromosome segregation. J Biol Chem 2002; 277: 49408-49416.
19. Miller DG, Adam MA, Miller AD. Gene transfer by retrovirus vectors occurs only in cells that are actively replicating at the time of infection. Mol Cell Biol 1990; 10: 4239-4242.
20. Cahill DP, Lengauer C, Yu J, Riggins GJ, Willson JK, Markowitz SD et al. Mutations of mitotic checkpoint genes in human cancers. Nature 1998; 392: 300-303.
21. Lengauer C, Kinzler KW, Vogelstein B. Genetic instabilities in human cancers. Nature 1998; 396: 643-649.
22. de la Hoz C, Baroja A. Proliferative behaviour of high-ploidy cells in two murine tumour lines. J Cell Sci 1993; 104: 31-36.
23. Li K, Lin S, Brunicardi FC, Seu P. Use of RNA interference to target cyclin E-overexpressing hepatocellular carcinoma. Cancer Res 2003; 63: 3593-3597.
24. Wilda M, Fuchs U, Wossmann W, Borkhardt A. Killing of leukemic cells with a BCR/ABL fusion gene by RNA interference (RNAi). Oncogene 2002; 21: 5716-5724.
25. Scherr M, Battmer K, Winkler T, Heidenreich O, Ganser A, Eder M. Specific inhibition of bcr-abl gene expression by small interfering RNA. Blood 2003; 101: 1566-1569.
26. Nieth C, Priebsch A, Stege A, Lage H. Modulation of the classical multidrug resistance (MDR) phenotype by RNA interference (RNAi). FEBS Lett 2003; 545: 144-150.
27. Duxbury MS, Ito H, Benoit E, Zinner MJ, Ashley SW, Whang EE. RNA interference targeting the M2 subunit of ribonucleotide reductase enhances pancreatic adenocarcinoma chemosensitivity to gemcitabine. Oncogene 2004; 23: 1539-1548.
28. Filleur S, Courtin A, Ait-Si-Ali S, Guglielmi J, Merle C, Harel-Bellan A et al. siRNA-mediated inhibition of vascular endothelial growth factor severely limits tumor resistance to antiangiogenic thrombospondin-1 and slows tumor vascularization and growth. Cancer Res 2003; 63: 3919-3922.
29. Muruve DA. The innate immune response to adenovirus vectors. Human Gene Ther 2004; 15: 1157-1166.
30. Zhou H, Xia XG, Xu Z. An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi. Nucleic Acids Res 2005; 33: Doi: 10.1093/nar/gni061.
31. Lakka SS, Gondi CS, Yanamandra N, Olivero WC, Dinh DH, Gujrati M et al. Inhibition of cathepsin B and MMP-9 gene expression in glioblastoma cell line via RNA interference reduces tumor cell invasion, tumor growth and angiogenesis. Oncogene 2004; 23: 4681-4689.
32. Izquierdo M, Cortes ML, Martin V, de Felipe P, Izquierdo JM, Perez-Higueras A et al. Gene therapy in brain tumours: Implications of the size of glioblastoma on its curability. Acta Neurochir Suppl 1997; 68: 111-117.