hNuf2 inhibition blocks stable kinetochore-microtubule attachment and induces mitotic cell death in HeLa cells

Journal of Cell Biology

Volumn 159, Issue 4, 2002, Pages 549-555

  DeLuca, Jennifer G ^a   Moree, Ben ^a   Hickey, Jennifer M ^a   Kilmartin, John V ^b   Salmon, E D ^a  

^a UNIVERSITY OF NORTH CAROLINA   (United States)

^b MRC LABORATORY OF MOLECULAR BIOLOGY   (United Kingdom)

Author keywords

HNuf2; Kinetochores; Microtubules; Mitosis; SiRNA

Indexed keywords

BUB1 SPINDLE CHECKPOINT PROTEIN; CALCIUM BINDING PROTEIN; CDCA1 PROTEIN, HUMAN; CELL CYCLE PROTEIN; CENTROMERE PROTEIN E; DYNEIN ADENOSINE TRIPHOSPHATASE; FUNGAL PROTEIN; MAD2L1 PROTEIN, HUMAN; NONHISTONE PROTEIN; NUCLEAR PROTEIN; PROTEIN KINASE; REPRESSOR PROTEIN; SMALL INTERFERING RNA;

ARTICLE; CELL DEATH; CENTROMERE; CYTOLOGY; FLOW CYTOMETRY; GENETICS; HELA CELL; HUMAN; METABOLISM; MICROTUBULE; MITOSIS; MITOSIS SPINDLE; PHYSIOLOGY; RNA INTERFERENCE;

CALCIUM-BINDING PROTEINS; CELL CYCLE PROTEINS; CELL DEATH; CHROMOSOMAL PROTEINS, NON-HISTONE; DYNEIN ATPASE; FLOW CYTOMETRY; FUNGAL PROTEINS; HELA CELLS; HUMANS; KINETOCHORES; MICROTUBULES; MITOSIS; MITOTIC SPINDLE APPARATUS; NUCLEAR PROTEINS; PROTEIN KINASES; REPRESSOR PROTEINS; RNA INTERFERENCE; RNA, SMALL INTERFERING;

EID: 0037175401     PISSN: 00219525     EISSN: 15408140     Source Type: Journal    
DOI: 10.1083/jcb.200208159     Document Type: Article

References (25)

1. Chen, Y., D.J. Riley, P. Chen, and W. Lee. 1997. HEC, a novel nuclear protein rich in leucine heptad repeats specifically involved in mitosis. Mol. Cell. Biol. 17:6049-6056.
2. Cimini, D., B. Howell, P. Maddox, A. Khodjakov, F. DeGrassi, and E.D. Salmon. 2001. Merotelic kinetochore orientation is a major mechanism of aneuploidy in mitotic mammalian tissue cells. J. Cell Biol. 153:517-527.
3. Elbashir, S.M., J. Harborth, W. Lendeckel, A. Yalcin, K. Weber, and T. Tuschl. 2001. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature. 411:494-498.
4. He, X., D.R. Rines, C.W. Espelin, and P.K. Sorger. 2001. Molecular analysis of kinetochore-microtubule attachment in budding yeast. Cell. 106:195-206.
5. Hoffman, D.B., C.G. Pearson, T.J. Yen, B.J. Howell, and E.D. Salmon. 2001. Microtubule-dependent changes in assembly of microtubule motor proteins and mitotic spindle checkpoint proteins at PtK1 kinetochores. Mol. Biol. Cell. 12:1995-2009.
6. Howe, M., K.L. McDonald, D.G. Albertson, and B.J. Meyer. 2001. HIM-10 is required for kinetochore structure and function on Caenorhabditis elegans holocentric chromosomes. J. Cell Biol. 153:1227-1238.
7. Howell, B.J., D.B. Hoffman, G. Fang, A.W. Murray, and E.D. Salmon. 2000. Visualization of Mad2 dynamics at kinetochores, along spindle fibers, and at spindle poles in living cells. J. Cell Biol. 150:1233-1250.
8. Howell, B.J., B.F. McEwen, J.C. Canman, D.B. Hoffman, E.M. Farrar, and E.D. Salmon. 2001. Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation. J. Cell Biol. 155:1159-1172.
9. Janke, C., J. Ortiz, J. Lechner, A. Shevchenko, A. Shevchenko, M.M. Magiera, C. Schramm, and E. Schiebal. 2001. The budding yeast proteins Spc24p and Spc25p interact with Ndc80p and Nuf2p at the kinetochore and are important for kinetochore clustering and checkpoint control. EMBO J. 20:777-791.
10. McEwen, B.F., G.K.T. Chan, B. Zubrowski, M.S. Savoian, M.T. Sauer, and T.J. Yen. 2001. CENP-E is essential for reliable bioriented spindle attachment, but chromosome alignment can be achieved via redundant mechanisms in mammalian cells. Mol. Biol. Cell. 12:2776-2789.
11. Milliband, D.N., L. Campbell, and K.G. Hardwick. 2002. The awesome power of multiple model systems: interpreting the complex nature of spindle checkpoint signaling. Trends Cell Biol. 12:205-209.
12. Mills, J.C., N.L. Stone, and R.N. Pittman. 1999. Extranuclear apoptosis. The role of the cytoplasm in the execution phase. J. Cell Biol. 146:703-708.
13. Mitchison, T.J., and E.D. Salmon. 1992. Poleward kinetochore fiber movement occurs during both metaphase and anaphase-A in newt lung cell mitosis. J. Cell Biol. 119:569-582.
14. Nabetani, A., T. Koujin, C. Tsutsumi, T. Haraguchi, and Y. Hiraoka. 2001. A conserved protein, Nuf2, is implicated in connecting the centromere to the spindle during chromosome segregation: a link between the kinetochore function and the spindle checkpoint. Chromosoma. 110:322-334.
15. Nabha, S.M., R.M. Mohammad, M.H. Dandashi, B.C. Gerard, A. Aboukameel, G.R. Pettit, and A.M. Al-Katib. 2002. Combretastatin-A4 prodrug induces mitotic catastrophe in chronic lymphocytic leukemia cell line independent of caspase activation and poly(ADP-ribose) polymerase cleavage. Clin. Cancer Res. 8:2735-2741.
16. Osborne, M.A., G. Schlenstedt, T. Jinks, and P.A. Silver. 1994. Nuf2, a spindle pole body-associated protein required for nuclear division in yeast. J. Cell Biol. 125:853-866.
17. Reno, F., E. Falcieri, F. Luchetti, S. Burattini, and S. Papa. 1997. Discrimination of apoptotic cells in flow cytometry using trypan blue and FDA. Eur. J. Histochem. 41:115-116.
18. Rieder, C.L. 1981. The structure of the cold-stable kinetochore fiber in metaphase PtK1 cells. Chromosoma. 84:145-158.
19. Rieder, C.L. 1982. The formation, structure, and composition of the mammalian kinetochore and kinetochore fiber. Int. Rev. Cytol 79:1-58.
20. Salmon, E.D., and C.L. Rieder. 1998. The vertebrate cell kinetochore and its roles during mitosis. Trends Cell Biol. 8:310-318.
21. Thrower, D.A., M.A. Jordan, and L. Wilson. 1996. Modulation of CENP-E organization at kinetochores by spindle microtubule attachment. CellMotil. Cytoskeleton. 35:121-133.
22. Waters, J.C., R. Chen, A.W. Murray, and E.D. Salmon. 1998. Localization of Mad2 to kinetochores depends upon microtubule attachment, not tension. J. Cell Biol. 141:1181-1191.
23. Wigge, P.A., and J.V. Kilmartin. 2001. The Ndc80p complex from Saccharomyces cerevisiae contains conserved centromere components and has a function in chromosome segregation. J. Cell Biol. 152:349-360.
24. Yao, X., A. Abrieu, Y. Zheng, K.F. Sullivan, and D.W. Cleveland. 2000. CENP-E forms a link between attachment of spindle microtubules to kinetochores and the mitotic checkpoint. Nat. Cell Biol. 2:484-491.
25. Zhang, J., and M. Xu. 2002. Apoptotic DNA fragmentation and tissue homeostasis. Trends Cell Biol. 12:84-89.