Chromatin dynamics in interphase cells revealed by tracking in a two-photon excitation microscope

Biophysical Journal

Volumn 89, Issue 6, 2005, Pages 4275-4285

  Levi, Valeria ^a   Ruan, QiaoQiao ^a   Plutz, Matthew ^a   Belmont, Andrew S ^a   Gratton, Enrico ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENOSINE TRIPHOSPHATE; ENHANCED GREEN FLUORESCENT PROTEIN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS; CHROMATIN; CONTROLLED STUDY; DIFFUSION; FLUORESCENCE MICROSCOPY; IN VIVO STUDY; INTERPHASE; MEASUREMENT; MOLECULAR DYNAMICS; MOTION; NONHUMAN; PREDICTION; TEMPERATURE DEPENDENCE;

ANIMALIA;

EID: 28444496753     PISSN: 00063495     EISSN: 00063495     Source Type: Journal    
DOI: 10.1529/biophysj.105.066670     Document Type: Article

References (37)

1. Marshall, W. F. 2002. Order and disorder in the nucleus. Curr. Biol. 12:R185-R192.
2. Belmont, A. S. 2001. Visualizing chromosome dynamics with GFP. Trends Cell Biol. 11:250-257.
3. Belmont, A. S., and A. F. Straight. 1998. In vivo visualization of chromosomes using lac operator-repressor binding. Trends Cell Biol. 8:121-124.
4. Marshall, W. F., A. Straight, J. F. Marko, J. Swedlow, A. Demburg, A. Belmont, A. W. Murray, D. A. Agard, and J. W. Sedat. 1997. Interphase chromosomes undergo constrained diffusional motion in living cells. Curr. Biol. 7:930-939.
5. Chubb, J. R., S. Boyle, P. Perry, and W. A. Bickmore. 2002. Chromatin motion is constrained by association with nuclear compartments in human cells. Curr. Biol. 12:439-445.
6. Vazquez, J., A. S. Belmont, and J. W. Sedat. 2001. Multiple regimes of constrained chromosome motion are regulated in the interphase Drosophila nucleus. Curr. Biol. 11:1227-1239.
7. Heun, P., T. Laroche, K. Shimada, P. Furrer, and S. M. Gasser. 2001. Chromosome dynamics in the yeast interphase nucleus. Science. 294:2181-2186.
8. Ferreira, J., G. Paolella, C. Ramos, and A. I. Lamond. 1997. Spatial organization of large-scale chromatin domains in the nucleus: a magnified view of single chromosome territories. J. Cell Biol. 139:1597-1610.
9. Spector, D. L. 2003. The dynamics of chromosome organization and gene regulation. Annu. Rev. Biochem. 72:573-608.
10. Tumbar, T., and A. S. Belmont. 2001. Interphase movements of a DNA chromosome region modulated by VP16 transcriptional activator. Nat. Cell Biol. 3:134-139.
11. Gasser, S. M. 2002. Visualizing chromatin dynamics in interphase nuclei. Science. 296:1412-1416.
12. Levi, V., Q. Ruan, and E. Gratton. 2005. 3-D particle tracking in a two-photon microscope: application to the study of molecular dynamics in cells. Biophys. J. 88:2919-2928.
13. Piston, D. 1996. Two-photon excitation microscopy. In Fluorescence Imaging Spectroscopy and Microscopy. X. Wang and B. Herman, editors. John Wiley & Sons, New York. 253-272.
14. Robinett, C. C., A. Straight, G. Li, C. Willhelm, G. Sudlow, A. Murray, and A. S. Belmont. 1996. In vivo localization of DNA sequences and visualization of large-scale chromatin organization using lac operator/repressor recognition. J. Cell Biol. 135:1685-1700.
15. Carpenter, A. E., and A. S. Belmont. 2004. Direct visualization of transcription factor-induced chromatin remodeling and cofactor recruitment in vivo. Methods Enzymol. 375:366-381.
16. Urlaub, G., P. J. Mitchell, E. Kas, L. A. Chasin, V. L. Funanage, T. T. Myoda, and J. Hamlin. 1986. Effect of gamma rays at the dihydrofolate reductase locus: deletions and inversions. Somat. Cell Mol. Genet. 12:555-566.
17. Platani, M., I. Goldberg, A. I. Lamond, and J. R. Swedlow. 2002. Cajal body dynamics and association with chromatin are ATP-dependent. Nat. Cell Biol. 4:502-508.
18. Kis-Petikova, K., and E. Gratton. 2004. Distance measurement by circular scanning of the excitation beam in the two-photon microscope. Microsc. Res. Tech. 63:34-49.
19. Berland, K. M., P. T. So, and E. Gratton. 1995. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. Biophys. J. 68:694-701.
20. Hicks, B. W., and K. J. Angelides. 1995. Tracking movements of lipids and Thy1 molecules in the plasmalemma of living fibroblasts by fluorescence video microscopy with nanometer scale precision. J. Membr. Biol. 144:231-244.
21. Wilson, K. M., I. E. Morrison, P. R. Smith, N. Femandez, and R. J. Cherry. 1996. Single particle tracking of cell-surface HLA-DR molecules using R-phycoerythrin labeled monoclonal antibodies and fluorescence digital imaging. J. Cell Sci. 109:2101-2109.
22. Daumas, F., N. Destainville, C. Millot, A. Lopez, D. Dean, and L. Salome. 2003. Confined diffusion without fences of a g-protein-coupled receptor as revealed by single particle tracking. Biophys. J. 84:356-366.
23. Saxton, M. J. 1993. Lateral diffusion in an archipelago. Single-particle diffusion. Biophys. J. 64:1766-1780.
24. Saxton, M. J. 1994. Anomalous diffusion due to obstacles: a Monte Carlo study. Biophys. J. 66:394-401.
25. Saxton, M. J. 1994. Single-particle tracking: models of directed transport. Biophys. J. 67:2110-2119.
26. Saxton, M. J. 1995. Single-particle tracking: effects of corrals. Biophys. J. 69:389-398.
27. Saxton, M. J. 1996. Anomalous diffusion due to binding: a Monte Carlo study. Biophys. J. 70:1250-1262.
28. Kusumi, A., C. Nakada, K. Ritchie, K. Murase, K. Suzuki, H. Murakoshi, R. S. Kasai, J. Kondo, and T. Fujiwara. 2005. Paradigm shift of the plasma membrane concept from the two dimensional continuum fluid to the partitioned fluid. High-speed single-molecule tracking of membrane molecules. Annu. Rev. Biophys. Biomol. Struct. 34:351-378.
29. Carmo-Fonseca, M., M. Platani, and J. R. Swedlow. 2002. Macromolecular mobility inside the cell nucleus. Trends Cell Biol. 12:491-495.
30. Muratani, M., D. Gerlich, S. M. Janicki, M. Gebhard, R. Eils, and D. L. Spector. 2002. Metabolic-energy-dependent movement of PML bodies within the mammalian cell nucleus. Nat. Cell Biol. 4:106-110.
31. Shav-Tal, Y., X. Darzacq, S. M. Shenoy, D. Fusco, S. M. Janicki, D. L. Spector, and R. H. Singer. 2004. Dynamics of single mRNPs in nuclei of living cells. Science. 304:1797-1800.
32. van Holde, K., and J. Zlatanova. 1996. What determines the folding of the chromatin fiber? Proc. Natl. Acad. Sci. USA. 93:10548-10555.
33. Cui, Y., and C. Bustamante. 2000. Pulling a single chromatin fiber reveals the forces that maintain its higher-order structure. Proc. Natl. Acad. Sci. USA. 97:127-132.
34. Belmont, A. S., S. Dietzel, A. C. Nye, Y. G. Strukov, and T. Tumbar. 1999. Large-scale chromatin structure and function. Curr. Opin. Cell Biol. 11:307-311.
35. Pestic-Dragovich, L., L. Stojiljkovic, A. A. Philimonenko, G. Nowak, Y. Ke, R. E. Settlage, J. Shabanowitz, D. F. Hunt, P. Hozak, and P. de Lanerolle. 2000. A myosin I isoform in the nucleus. Science. 290:337-341.
36. Philimonenko, V. V., J. Zhao, S. Iben, H. Dingova, K. Kysela, M. Kahle, H. Zentgraf, W. A. Hofmann, P. de Lanerolle, P. Hozak, and I. Grummt. 2004. Nuclear actin and myosin I are required for RNA polymerase I transcription. Nat. Cell Biol. 6:1165-1172.
37. Saxton, M. J., and K. Jacobson. 1997. Single-particle tracking: applications to membrane dynamics. Annu. Rev. Biophys. Biomol. Struct. 26:373-399.