Single particle tracking of cell-surface HLA-DR molecules using R-phycoerythrin labeled monoclonal antibodies and fluorescence digital imaging

Journal of Cell Science

Volumn 109, Issue 8, 1996, Pages 2101-2109

  Wilson, Keith M ^a   Morrison, Ian E G ^a   Smith, Patricia R ^a   Fernandez, Nelson ^a   Cherry, Richard J ^a  

^a UNIVERSITY OF ESSEX   (United Kingdom)

Author keywords

Fluorescence imaging; MHC; Phycobiliprotein; Receptor mobility

Indexed keywords

HLA DR ANTIGEN; MEMBRANE ANTIGEN; MONOCLONAL ANTIBODY; PHYCOERYTHRIN;

ANTIGEN PRESENTATION; ARTICLE; DIFFUSION COEFFICIENT; FIBROBLAST; FLUORESCENCE; GENETIC TRANSFECTION; MOLECULAR DYNAMICS; PRIORITY JOURNAL; T LYMPHOCYTE;

ANTIBODIES, MONOCLONAL; B-LYMPHOCYTES; FLOW CYTOMETRY; HLA-DR ANTIGENS; HUMANS; IMAGE ENHANCEMENT; PHYCOERYTHRIN; RECEPTORS, ANTIGEN, T-CELL;

EID: 0029815535     PISSN: 00219533     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (48)

1. Abbas, A. K., Lichtman, A. H. and Pober, J. S. (1994). Antigen processing and presentation to T lymphocytes. In Cell and Molecular Immunology (ed. Pober, J. S.), pp. 116-134. W. B. Saunders Company, London.
2. Anderson, C. M., Georgiou, G. G., Morrison, I. E. G., Stevenson, G. V. W. and Cherry, R. J. (1992). Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera: low-density lipoprotein and influenza virus receptor mobility at 4°C. J. Cell Sci. 101, 415-425.
3. Bene, L., Balazs, M., Matko, J., Most, J., Dierich, M. P., Szollosi, J. and Damjanovich, S. (1994). Lateral organization of the ICAM-1 molecule at the surface of human lymphoblasts: a possible model for its co-distribution with the IL-2 receptor, class I and class II HLA molecules. Eur. J. Immunol. 24, 2115-2123.
4. Bevington, P. R. (1969). Data Reduction and Error Analysis for the Physical Sciences (ed. P. R. Bevington), pp. 204-246. McGraw-Hill, New York.
5. deBrabrander, M., Nuydens, R., Ishihara, A. Holifield, B. Jacobson, K. and Geerts, H. (1991). Lateral diffusion and retrograde movements of individual cell surface components on single motile cells observed with Nanovid microscopy. J. Cell Biol. 112, 1143-1150.
6. Bjorkman, P. J., Saper, M. A., Saraqui, B., Bennett, W. S., Strominger, J. L., and Wilet, D. C. (1987). Structure of the human class I histocompatibility antigen, HLA-A2. Nature 329, 506-510.
7. Brown, J. H., Jardetzky, T. S., Gorga, J. C., Stern, L. J., Urban, R. G., Strominger, J. L. and Wiley, D. C. (1993). Three-dimensional structure of the human class II histocompatibility antigen HLA-DR1. Nature 364, 33-39.
8. Capps, G. G., Robinson, B. E., Lewis, R. D. and Zuniga, M. C. (1993). In vivo dimeric association of class IMHC heavy chains J. Immunol. 151, 159-169.
9. Clark, E. A. and Ledbetter, J. A. (1994). How B and T cells talk to each other. Nature 367, 425-428.
10. Chakrabarti, A., Matko, J., Rahman, N. A., Barisas, B. G. and Edidin, M. (1992). Self-association of class I major histocompatibility complex molecules in liposomes and cell surface membranes. Biochemistry 31, 7182.
11. Damjanovich, S., Vereb, G., Schapera, A., Jenei, A., Matko, J., Starink J. P. P., Fox, G. Q., Arndtjovin, D. J. and Jovin, T. M. (1995) Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells. Proc. Nat. Acad. Sci. USA 92, 1122-1126.
12. Davis, M. M. and Chien, Y. (1993). Topology and affinity of T-cell receptor mediated recognition of peptide-MHC complexes. Curr. Opin. Immunol. 5, 45-40.
13. Dustin, M. L. and Springer, T. A. (1989). T-cell receptor cross-linking transiently stimulates adhesiveness throgh LFA-1. Nature 341, 619-625.
14. Dodi, A., Brett, S., Nordeng, T., Sidhu, S., Batchelor, R. J., Lombardi, G., Bakke, O. and Lechler, R. I. (1994). The invariant chain inhibits presentation of endogeneous antigens by a human fibroblast cell line. Eur. J. Immunol. 24, 1632-1639.
15. Edidin, M. (1990). Molecular associations of major histocompatibility complex antigens. Biochem. Soc. Trans. 18, 152-154.
16. Edidin, M. (1993). Patches and fences: probing for plasma membrane domains. J. Cell Sci. Suppl. 17, 165-169.
17. Edidin, M., Zuniga, M. C. and Sheetz, M. P. (1994). Truncation mutants define and locate cytoplasmic barriers to lateral mobility of membrane glycoproteins. Proc. Nat. Acad Sci USA 91, 3378-3382.
18. Fernandez, N., Kurpisz, M., Labeta, M., Sachs, J. and Pawelec, G. (1992). A ligand-epitope in vitro analysis of major histocompatibility determinants expressed on B and T lymphocytes. Immunology 77, 116-122.
19. Ghosh, R. N. and Webb, W. W. (1994). Automated detection and tracking of individual and clustered cell surface low density lipoprotein receptor molecules Biophys. J. 66, 1301-1318.
20. Glazer, A. N. (1983). Comparative biochemistry of photosynthetic light-harvesting systems. Annu. Rev. Biochem. 52, 125-157.
21. Gross, D. J. and Webb, W. W. (1988). Cell surface clustering and mobility of the liganded LDL receptor measured by digital video fluorescence microscopy. In Spectroscopic Membrane Probes, vol. II (ed. L. M. Loew), pp. 19-45. Boca Raton, CRC Press.
22. Harlow, E. and Lane, D. (1989). Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
23. Hicks, B. W. and Angelides, K. J. (1995). Tracking movements of lipids and Thy1 molecules in the plasmalemma of living fibroblasts by fluorescence video microscopy with nanometer scale precision. J. Membr. Biol. 144, 231-244.
24. Janeway, C. A. (1992). The T cell receptor as a multicomponent signalling machine: CD4/CD8 coreceptors and CD45 in T cell activation. Annu. Rev. Immunol. 10, 645-674.
25. Kupfer, A. and Singer, S. J. (1988). Molecular dynamics in the membranes of helper T cells. Proc. Nat. Acad. Sci. USA 85, 8216-8220.
26. Kusumi, A., Sako, Y. and Yamamoto, M. (1993). Confined lateral diffusion of membrane receptors as studied by single particle tracking (Nanovid microscopy). Effects of calcium-induced differentiation in cultured epithelial cells. Biophys. J. 65, 2021-2040.
27. Lee, G. M., Ishihara, A. and Jacobson, K. (1991). Direct observation of Brownian motion of lipids in membranes. Proc. Nat. Acad. Sci. USA 88, 6274-7278.
28. Lightstone, L., Hargreaves R., Bobek, G., Lombardi, G., Stauss and Lechler, R. (1995). Absence of invariant chain (Ii) alters the repertoire of peptides displayed by class II. Immunology 86, Suppl. 1, 79.
29. Lodish H., Baltimore, D., Berk, A., Zipursky S. L., Matsudaira, P. and Darnell, J. (1995). Manipulating cells and virus in culture. In Molecular Cell Biology (ed. J. Darnell), pp. 189-220. Scientific American Books, W. H. Freeman, New York.
30. Matko, J., Bushkin, Y., Wei, T. and Edidin, M. (1994). Clustering of class-I HLA molecules on the surfaces of activated and transformed human cells. J. Immunol. 152, 3353-3360.
31. Matko, J., Jenei, A., Wei, T. and Edidin, M. (1995). Luminescence quenching by long range electron transfer: a probe of protein clustering and conformation at the cell surface. Cytometry 19, 191-200.
32. Morrison, I. E. G., Anderson, C. M., Georgiou, G. N., Stevenson, G. V. W. and Cherry, R. J. (1994). Analysis of receptor clustering on cell surfaces by imaging fluorescent particles. Biophys. J. 67, 1280-1290.
33. Oi, V. T., Glazer, A. N. and Stryer, L. (1982). Fluorescent phycobiliprotein conjugates for analysis of cells and molecules. J. Cell Biol. 93, 981-986.
34. Philpott, C. J., Rahman, N. A., Kenny, N., Londo, T. R., Young, R. M., Barisas, B. G. and Roess, T. R. (1995). Rotational dynamics of luteinizing hormone receptors and MHC class I antigens on murine Leydig cells. Biochem. Biophys. Acta 1235, 62-68.
35. Qian, H. Sheetz, M. P. and Elson, E. L. (1991). Single particle tracking analysis of diffusion and flow in two-dimensional systems. Biophys. J. 60, 910-921.
36. Rotzschke, O., Falk, K., Deres, K., Schild, H., Nordal, M., Metzger, J., Jung, G. and Rammensee, H.-G. (1990). Isolation and analysis of naturally processed viral peptides as recognised by cytotoxic T cells. Nature 348, 252-254.
37. Sakihama, T., Smolyer, A. and Reinherz, E. L. (1995). Molecular recognition of antigen involves lattice formation between CD4, MHC class II and TCR molecules. Immunol Today 16, 581-587.
38. Sako, Y. and Kusumi, A. (1994). Compartmentalised structure of the plasma membrane for receptor movements as revealed by a nanometer-level motion analysis. J. Cell Biol. 125, 1251-1264.
39. Sako, Y. and Kusumi, A. (1995). Barriers for lateral diffusion of transferrin receptor in the plasma membrane as characterized by receptor dragging by laser tweezers: fence versus tether. J. Cell Biol. 129, 1559-1574.
40. Saxton, M. J. (1993). Lateral diffusion in an archipelago: single particle diffusion. Biophys. J. 64, 1766-1780.
41. Saxton, M. J. (1994). Single particle tracking: models of directed transport Biophys. J. 67, 2110-2119.
42. Saxton, M. J. (1995). Single particle tracking: effects of corrals. Biophys. J. 69, 389-398.
43. Schafer, P. H. and Pierce, S. K. (1994). Evidence for dimers of MHC class II molecules in B lymphocytes and their role in low affinity T cell responses. Immunity 1, 699-707.
44. Sheetz, M. P., Turney, S., Qian, H. and Elson, E. L. (1989). Nanometer-level analysis demonstrates that lipid flow does not drive membrane glycoprotein movement. Nature 340, 284-288.
45. Simson R., Sheets, E. D. and Jacobson, K. (1995). Detection of temporary lateral confinement of membrane proteins using single particle tracking. Biophys. J. 69, 989-993.
46. Smith, P. R., Wilson, K. M., Morrison, I. E. G., Cherry R. J. and Fernandez, N. (1996). Imaging of individual cell surface MHC antigens using fluorescent particles. In MHC Biochemistry and Genetics (ed. N. Fernandez and G. Butcher). Practical Approach Series, Oxford University Press (in press).
47. Stossel, T. P. (1994). The machinery of cell crawling. Scient. Am. 271, 40-47.
48. Wang, Y., Silverman, J. D. and Cao, L. (1994). Single particles tracking of surface receptor movement during cell division. J. Cell Biol. 127, 963-971.