3-D particle tracking in a two-photon microscope: Application to the study of molecular dynamics in cells

Biophysical Journal

Volumn 88, Issue 4, 2005, Pages 2919-2928

  Levi, Valeria ^a   Ruan, QiaoQiao ^a   Gratton, Enrico ^a  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLYCEROL; MICROSPHERE; POLYSTYRENE; PROTEIN;

ARTICLE; ARTIFACT REDUCTION; CALCULATION; CELL; DEPTH PERCEPTION; DIFFUSION COEFFICIENT; FIBROBLAST; FLUORESCENCE; INJURY; LASER; LIGHT; LIVING CELL; MEASUREMENT; MICROSCOPE; MOLECULAR DYNAMICS; MOTION; NANOTECHNOLOGY; PARTICLE POSITION; PHAGOCYTOSIS; PHOTODAMAGE; PIEZOELECTRICITY; POSITION; STOKES EINSTEIN RELATIONSHIP; TECHNIQUE; THEORY; THREE DIMENSIONAL IMAGING; THREE DIMENSIONAL TRACKING; TWO PHOTON MICROSCOPE; VISCOSITY;

EID: 22144445955     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1529/biophysj.104.044230     Document Type: Article

References (35)

1. Anderson, C. M., G. N. Georgiern, I. E. G. Morrison, G. V. W. Stevenson, and R. J. Cherry. 1992. Tracking of cell surface receptors by fluorescence digital imaging microscopy using a charge-coupled device camera: low-density lipoprotein and influenza virus receptor mobility at 4°C. J. Cell Sci. 101:415-425.
2. Arlein, W. J., J. D. Shearer, and M. D. Caldwell. 1998. Continuity between wound macrophage and fibroblast phenotype: analysis of wound fibroblast phagocytosis. Am. J. Physiol. 275:1041-1048.
3. Atkins, P. W. 1978. Physical Chemistry. W. H. Freeman and Company, San Francisco.
4. Berland, K. M., P. T. So, and E. Gratton. 1995. Two-photon fluorescence correlation spectroscopy: method and application to the intracellular environment. Biophys. J. 68:694-701.
5. Blocker, A., F. F. Severin, J. K. Burkhardt, J. B. Bingham, H. Yu, J. C. Olivo, T. A. Schroer, A. A. Hyman, and G. Griffiths. 1997. Molecular requirements for bi-directional movement of phagosomes along microtubules. J. Cell Biol. 137:113-129.
6. Bornfleth, H., P. Edelmann, D. Zink, T. Cremer, and C. Cremer. 1999. Quantitative motion analysis of subchromosomal foci in living cells using four-dimensional microscopy. Biophys. J. 77:2871-2886.
7. Caspi, A., O. Yeger, I. Grosheva, A. D. Bershadsky, and M. Elbaum. 2001. A new dimension in retrograde flow: centripetal movement of engulfed particles. Biophys. J. 81:1990-2000.
8. Chirico, G., C. Fumagalli, and G. Baldini. 2002. Trapped Brownian motion in single- and two-photon excitation fluorescence correlation experiments. J. Phys. Chem. B. 106:2508-2519.
9. Dahan, M., S. Levi, C. Luccardini, P. Rostaing, B. Riveau, and A. Triller. 2003. Diffusion dynamics of glycine receptors revealed by single-quantum dot tracking. Science. 302:442-445.
10. Daumas, F., N. Destainville, C. Millot, A. Lopez, D. Dean, and L. Salome. 2003. Confined diffusion without fences of a g-protein-coupled receptor as revealed by single particle tracking. Biophys. J. 84:356-366.
11. Fujiwara, T., K. Ritchie, H. Murakoshi, K. Jacobson, and A. Kusumi. 2002. Phospholipids undergo hop diffusion in compartmentalized cell membrane. J. Cell Biol. 157:1071-1081.
12. Goulian, M., and S. M. Simon. 2000. Tracking single proteins within cells. Biophys. J. 79:2188-2198.
13. Haughland, R. P. 1998. Handbook of Fluorescent Probes and Research Chemicals. T. Z. Spence, editor. Molecular Probes, Eugene, OR.
14. Hicks, B. W., and K. J. Angelides. 1995. Tracking movements of lipids and Thy1 molecules in the plasmalemma of living fibroblasts by fluorescence video microscopy with nanometer scale precision. J. Membr. Biol. 144:231-244.
15. Kis-Petikova, K., and E. Gratton. 2004. Distance measurement by circular scanning of the excitation beam in the two-photon microscope. Microsc. Res. Tech. 63:34-49.
16. Kubitscheck, U., O. Kuckmann, T. Kues, and R. Peters. 2000. Imaging and tracking of single GFP molecules in solution. Biophys. J. 78:2170-2179.
17. Kues, T., R. Peters, and U. Kubitscheck. 2001. Visualization and tracking of single protein molecules in the cell nucleus. Biophys. J. 80:2954-2967.
18. Lee, W., and C. A. McCulloch. 1997. Deregulation of collagen phagocytosis in aging human fibroblasts: effects of integrin expression and cell cycle. Exp. Cell Res. 237:383-393.
19. Levi, V., Q. Ruan, K. Kis-Petikova, and E. Gratton. 2003. Scanning FCS, a novel method for three-dimensional particle tracking. Biochem. Soc. Trans. 31:997-1000.
20. McKeown, M., G. Knowles, and C. A. McCulloch. 1990. Role of the cellular attachment domain of fibronectin in the phagocytosis of beads by human gingival fibroblasts in vitro. Cell Tissue Res. 262:523-530.
21. Murase, K., T. Fujiwara, Y. Umemura, K. Suzuki, R. Iino, H. Yamashita, M. Saito, H. Murakoshi, K. Ritchie, and A. Kusumi. 2004. Ultrafine membrane compartments for molecular diffusion as revealed by single molecule techniques. Biophys. J. 86:4075-4093.
22. Noji, H., R. Yasuda, M. Yoshida, and K. Kinosita. 1997. Direct observation of the rotation of F1-ATPase. Nature. 386:299-302.
23. Piston, D. W. 1996. Two-photon excitation microscopy. In Fluorescence Imaging Spectroscopy and Microscopy. X. F. Wang and B. Herman, editors. John Wiley & Sons, New York. 253-272.
24. Rabinovitch, M. 1995. Professional and non-professional phagocytes: an introduction. Trends Cell Biol. 5:85-87.
25. Saxton, M. J. 1994. Anomalous diffusion due to obstacles: a Monte Carlo study. Biophys. J. 66:394-401.
26. Saxton, M. J., and K. Jacobson. 1997. Single-particle tracking: applications to membrane dynamics. Annu. Rev. Biophys. Biomol. Struct. 26:373-399.
27. Schroeder, F., and D. A. Kinden. 1983. Measurement of phagocytosis using fluorescent latex beads. J. Biochem. Biophys. Methods. 8:15-27.
28. Segal, G., W. Lee, P. D. Arora, M. McKee, G. Downey, and C. A. McCulloch. 2001. Involvement of actin filaments and integrins in the binding step in collagen phagocytosis by human fibroblasts. J. Cell Sci. 114:119-129.
29. Seisenberger, G., M. U. Ried, T. Endress, H. Buning, M. Hallek, and C. Brauchle. 2001. Real-time single-molecule imaging of the infection pathway of an adeno-associated virus. Science. 294:1929-1932.
30. Smith, P. R., I. E. Morrison, K. M. Wilson, N. Fernandez, and R. J. Cherry. 1999. Anomalous diffusion of major histocompatibility complex class I molecules on HeLa cells determined by single particle tracking. Biophys. J. 76:3331-3344.
31. Speidel, M., A. Jonás, and E. L. Florin. 2003. Three-dimensional tracking of fluorescent nanoparticles with subnanometer precision by use of off-focus imaging. Opt. Lett. 69:69-71.
32. Thomann, D., D. R. Rines, P. K. Sorger, and G. Danuser. 2002. Automatic fluorescent tag detection in 3D with super-resolution: application to the analysis of chromosome movement. J. Microsc. 208:49-64.
33. Toyohara, A., and K. Inaba. 1989. Transport of phagosomes in mouse peritoneal macrophages. J. Cell Sci. 94:143-153.
34. Wilson, K. M., I. E. Morrison, P. R. Smith, N. Fernandez, and R. J. Cherry. 1996. Single panicle tracking of cell-surface HLA-DR molecules using R-phycoerythrin labeled monoclonal antibodies and fluorescence digital imaging. J. Cell Sci. 109:2101-2109.
35. Yildiz, A., J. N. Forkey, S. A. McKinney, T. Ha, Y. E. Goldman, and P. R. Selvin. 2003. Myosin V walks hand-over-hand: single fluorophore imaging with 1.5-nm localization. Science. 300:2061-2065.