Real-time polymerase chain reaction quantification of Phytophthora capsici in different pepper genotypes

Phytopathology

Volumn 95, Issue 12, 2005, Pages 1423-1429

  Silvar, C ^a   Diaz J ^a   Merino, F ^a  

^a UNIVERSITY OF A CORUÑA   (Spain)

Author keywords

Capsicum annuum; PI201234; Serrano Criollo de Morelos 331; Yolo Wonder

Indexed keywords

CAPSICUM ANNUUM; PHYTOPHTHORA; PHYTOPHTHORA CAPSICI;

EID: 28044440886     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/PHYTO-95-1423     Document Type: Article

References (59)

1. Alcantara, T. P., and Bosland, P. W. 1994. An inexpensive disease screening technique for foliar blight of chili pepper seedlings. HortScience 29:1182-1183.
2. Bates, J. A., Taylor, E. J. A., Kenyon, D. M., and Thomas, J. E. 2001. The application of real-time PCR to the identification, detection and quantification of Pyrenophora species in barley seed. Mol. Plant Pathol. 2:49-57.
3. Bermingham, S., Maltby, L., and Cooke, R. C. 1995. A critical assessment of the validity of ergosterol as an indicator of fungal biomass. Mycol. Res. 99:479-484.
4. Black, L. L., and Berke, T. 1998. Breeding for Phytophthora resistance in pepper. Xth Eucarpia Meeting on Genetics and Breeding of Capsicum and Eggplant. Avignon, France.
5. Böhm, J., Hahn, A., Schubert, R., Bahnweg, G., Adler, N., Nechwatal, J., Oehlmann, R., and Obwald, W. 1999. Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophthora infestans and Phytophthora citricola in their respective host plants. J. Phytopathol. 147:409-416.
6. Bonants, P. J. M., van Gent-Pelzer, M. P. E., Hoofman, R., Cooke, D. E. L., Guy, D. C., and Duncan, J. M. 2004. A combination of baiting and different PCR formats, including measurement of real-time quantitative fluorescence, for the detection of Phytophthora fragariae in strawberry plants. Eur. J. Plant Pathol. 110:689-702.
7. Bosland, P. W., and Lindsey, D. L. 1991. A seedling screen for Phytophthora root rot of pepper, Capsicum annuum. Plant Dis. 75:1048-1050.
8. Bowers, J. H., and Mitchell, D. J. 1991. Relationships between inoculum level of Phytophthora capsici and mortality of pepper. Phytopathology 81:178-184.
9. Brouwer, M., Lievens, B., van Hemelrijck, W., van den Ackerveken, G., Cammue, B. P. A., and Thomma, B. P. H. J. 2003. Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR. FEMS Microbiol. Lett. 228:241-248.
10. Conover, W. J. 1980. Practical Nonparametric Statistics. John Wiley & Sons, Inc., New York.
11. Cooke, D. E. L., Drenth, A., Duncan, J. M., Wagels, G., and Brasier, C. M. 2000. A molecular phylogeny of Phytophthora and related Oomycetes. Fungal Genet. Biol. 30:17-32.
12. Dan, H., Ali-Khan, A. T., and Robb, J. 2001. Use of quantitative PCR diagnostics to identify tolerance and resistance to Verticillium dahliae in potato. Plant Dis. 85:700-705.
13. Dewey, F. M., Thornton, C. R., and Gilligan, C. A. 1997. Use of monoclonal antibodies to detect, quantify, and visualize fungi in soil. Adv. Bot. Res. 24:276-308.
14. Estrada, B., Bernal, M. A., Díaz, J., Pomar, F., and Merino, F. 2000. Fruit development in Capsicum annuum: Changes in capsaicin, lignin, free phenolics and peroxidase patterns. J. Agric. Food Chem. 48:6234-6239.
15. Filion, M., St.-Arnaud, M., and Jabaji-Hare, S. H. 2003. Quantification of Fusarium solani f. sp. phaseoli in mycorrhizal bean plants and surrounding mycorrhizosphere soil using real-time polymerase chain reaction and direct isolations on selective media. Phytopathology 93:229-235.
16. Filion, M., St-Arnaud, M., and Jabaji-Hare, S. H. 2003. Direct quantification of fungal DNA from soil substrate using real-time PCR. J. Microbiol. Methods 53:67-76.
17. Gachon, C., and Saindrenan, P. 2004. Real-time PCR monitoring of fungal development in Arabidopsis thaliana infected by Alternaria brassicicola and Botrytis cinerea. Plant Physiol. Biochem. 42:367-371.
18. Gardner, R. M., Tindall, G. W., Cline, S. M., and Brown, K. L. 1993. Ergosterol determination activated sludge and its application as a biochemical marker for monitoring fungal biomass. J. Microbiol. Methods 17:49-60.
19. Gil Ortega, R., Español, C. P., and Zueco, J. C. 1991. Genetics of resistance to Phytophthora capsici in the pepper line "SCM-334". Plant Breed. 107:50-55.
20. Gil Ortega, R., Español, C. P., and Zueco, J. C. 1995. Interactions in the pepper-Phytophthora capsici system. Plant Breed. 114:74-77.
21. Gretenkort, M. A., and Ingram, D. S. 1993. The use of ergosterol as a quantitative measure of the resistance of cultured tissues of Brassica napus spp. oleifera to Leptosphaeria maculans. J. Phytopathol. 138:217-224.
22. Harrison, J. G., Barker, H., Lowe, R., and Rees, E. A. 1990. Estimation of amounts of Phytophthora infestans mycelium in leaf tissue by enzyme-linked immunosorbent assay. Plant Pathol. 34:557-564.
23. Hayden, K. J., Rizzo, D., Tse, J., and Garbelotto, M. 2004. Detection and quantification of Phytophthora ramorum from California forests using a real-time polymerase chain reaction assay. Phytopathology 94:1075-1083.
24. Hoffman, T., Schmidt, J. S., Zheng, X., and Bent, A. F. 1999. Isolation of ethylene-insensitive soybean mutants that are altered in pathogen susceptibility and gene-for-gene disease resistance. Plant Physiol. 119:935-950.
25. Holland, P. M., Abramson, R. D., Watson, R., and Gelfand, D. H. 1991. Detection of specific polymerase chain reaction product by utilizing the 5′-3′exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88:7276-7280.
26. Ippolito, A., Schena, L., and Nigro, F., Ligorio, V. S., and Yaseen, T. 2004. Real-time detection of Phytophthora nicotianae and P. citrophthora in citrus roots and soil. Eur. J. Plant Pathol. 110:833-843.
27. Jung, H. W., and Hwang, B. K. 2000. Pepper gene encoding a basic β-1,3-glucanase is differentially expressed in pepper tissues upon pathogen infection and ethephon or methyl jasmonate treatment. Plant Sci. 156:23-34.
28. Jung, H. W., Kim, W., and Hwang, B. K. 2003. Three pathogen-inducible genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, and environmental stresses. Plant Cell Environ. 26:915-928.
29. Karpovich-Tate, N., Spanu, P., and Dewe, F. M. 1998. Use of monoclonal antibodies to determine biomass of Cladosporium fulvum in infected tomato leaves. Mol. Plant-Microbe Interact. 11:710-716.
30. Larkin, R. P., Ristaino, J. B., and Campbell, C. L. 1995. Detection and quantification of Phytophthora capsici in soil. Phytopathology 85:1057-1063.
31. Maniatis, T., Fritsch, E. F., and Sambrook, J. 1982. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
32. Martin, F., Delaruelle, A., and Hilberty, J. L. 1990. An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol. Res. 94:1059-1064.
33. McCartney, H. A., Foster, S. J., Fraaije, B. A., and Ward, E. 2003. Molecular diagnostics for fungal plant pathogens. Pest. Manage. Sci. 59:129-142.
34. Mercado-Blanco, J., Collado-Romero, M., Parrilla-Araujo, S., Rodríguez-Jurado, D., and Jiménez-Díaz, R. M. 2003. Quantitative monitoring of colonization of olive genotypes by Verticillium dahliae pathotypes with real-time polymerase chain reaction. Physiol. Mol. Plant Pathol. 63:91-105.
35. Miller, S. A. 1996. Detecting propagules of plant pathogenic fungi. Adv. Bot. Res. 23:73-102.
36. Morrison, T. B., Weis, J. J., and Wittwer, C. T. 1998. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. BioTechniques 24:954-962.
37. Mumford, R. A., Walsh, K., Barker, I., and Boonham, N. 2000. Detection of potato mop top virus and tobacco rattle virus using a multiplex real-time fluorescent reverse-transcription polymerase chain reaction assay. Phytopathology 90:448-453.
38. Newton, A. C., and Reglinski, T. 1993. An enzyme-linked immunosorbent assay for quantifying mildew biomass. J. Plant. Dis. Prot. 100:176-179.
39. Nuez, F., Gil, R., and Costa J. 1996. El cultivo de pimientos, chiles y ajíes. Ediciones Mundi-Prensa, Madrid.
40. Pacovsky, R. S., and Bethlenfalvay, G. J. 1982. Measurement of the extraradical mycelium of a vesicular-arbuscular mycorrhizal fungus in soil by chitin determination. Plant Soil 68:143-147.
41. Palloix, A., Daubeze, A. M., and Pochard, E. 1988. Phytophthora root rot of pepper. Influence of host genotype and pathogen strain on the inoculum density-disease severity relationships. J. Phytopathol. 123:25-33.
42. Pegg, G. F., and Brady, B. L. 2002. Verticillium Wilts. CAB International, Wallingford, UK.
43. Plassard, C. S., Mousain, D. G., and Salsac, L. E. 1982. Estimation of mycelia growth of basidiomycetes by means of chin determination. Phytochemistry 21:345-348.
44. Pomar, F., Bernal, M. A., Collar, J., Díaz, I., Caramelo, C., Gayoso, C., Novo, M., Prego, C., Saavedra, A., Silvar, C., and Merino, F. 2001. A survey of "Tristeza" of pepper in Galicia and the fungal pathogens causing the disease. Capsicum Eggplant Newsl. 20:90-93.
45. Qi, M., and Yang, Y. 2002. Quantification of Magnaporthe grisea during infection of rice plants using real-time polymerase chain reaction and northern blot/phosphimaging analyses. Phytopathology 92:870-876.
46. Ririe, K. M., Rasmussen, R. P., and Wittwer, C. T. 1997. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 245:154-160.
47. Schaad, N. W., Frederick, R. D., Shalev, A., Shaw, J., Schneider, W. L., Hickson, R., Petrillo, M. D., and Luster, D. G. 2003. Advances in molecular-based diagnostics in meeting crop biosecurity and phytosanitary issues. Annu. Rev. Phytopathol. 41:305-324.
48. Schena, L., Nigro, F., Ippolito, A., and Gallitelli, D. 2004. Real-time quantitative PCR: A new technology to detect and study phytopathogenic and antagonistic fungi. Eur. J. Plant Pathol. 110:893-908.
49. Shin, R., Lee, G. J., Park, C. J., Kim, T. Y., You, J. S., Nam, Y. W., and Paek, K. H. 2001. Isolation of pepper mRNAs differentially expressed during the hypersensitive response to tobacco mosaic virus and characterization of a proteinase inhibitor gene. Plant Sci. 161:727-737.
50. Silvar, C., Duncan, J. M., Cooke, D. E. L., Williams, N. A., Díaz, J., and Merino, F. 2005. Development of specific PCR primers for identification and detection of Phytophthora capsici Leon. Eur. J. Plant Pathol. 112:43-52.
51. Srivastava, A. K., and Arora, D. K. 1997. Evaluation of a polyclonal antibody immunoassay for detection and quantification of Macrophomina phaseolina. Plant Pathol. 46:785-794.
52. Takenaka, S. 1995. Dynamics of fungal pathogens in host plant tissues. Can. J. Bot. 73:S1275-S1283.
53. Thomma, B. P. H. J., Tadesse, Y. S. H., Jacobs, M., and Browkaert, W. F. 1999. Disturbed correlation between fungal biomass and β-glucuronidase activity in infections of Arabidopsis thaliana with transgenic Alternaria brassicicola. Plant Sci. 148:31-36.
54. Tyagi, S., and Kramer, F. R. 1996. Molecular beacons: Probes that fluoresce upon hybridization. Nat. Biotechnol. 14:303-308.
55. Vandemark, G. J., and Barker, B. M. 2003. Quantifying Phytophthora medicaginis in susceptible and resistant alfalfa with a real-time fluorescent assay. J. Phytopathol. 151:577-583.
56. Ward, E., Tahiri-Alaouri, A., and Antoniw, J. F. 1998. Applications of PCR in fungal-plant interactions. Pages 289-307 in: Application of PCR in Mycology. P. D. Bridge, D. K. Arora, C. A. Reddy, and R. P. Elander, eds. CAB International, London.
57. Whitcombe, D., Theaker, J., Guy, S. P., Brown, T., and Little, S. 1999. Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 17:804-807.
58. Winton, L. M., Manter, D. K., Stone, J. K., and Hansen, E. A. 2003. Comparison of biochemical, molecular, and visual methods to quantify Phaeocryptopus gaeumannii in Douglas-Fir foliage. Phytopathology 93:121-126.
59. Zhou, X. G., and Everts, K. L. 2004. Quantification of root and stem colonization of watermelon by Fusarium oxysporum f. sp. niveum and its use in evaluating resistance. Phytopathology 94:832-841.