Quantification of disease progression of several microbial pathogens on Arabidopsis thaliana using real-time fluorescence PCR

FEMS Microbiology Letters

Volumn 228, Issue 2, 2003, Pages 241-248

  Brouwer, Margreet ^a   Lievens, Bart ^a,b   Van Hemelrijck, Wendy ^a   Van Den Ackerveken, Guido ^c   Cammue, Bruno P A ^a   Thomma, Bart P H J ^a,d  

^a UNIVERSITY OF LEUVEN   (Belgium)

^b Scientia Terrae Research Institute   (Belgium)

^c UTRECHT UNIVERSITY   (Netherlands)

^d WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Alternaria brassicicola; Arabidopsis thaliana; Botrytis cinerea; Erwinia carotovora; LightCycler; Peronospora parasitica; Pseudomonas syringae; SYBR Green I

Indexed keywords

DNA;

ANALYTIC METHOD; ARABIDOPSIS; ARTICLE; DISEASE COURSE; FLUORESCENCE; GENOTYPE; HOST PATHOGEN INTERACTION; INOCULATION; MICROBIAL BIOMASS; MICROORGANISM DETECTION; PATHOGENESIS; PLANT GENETICS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS;

ALTERNARIA; ALTERNARIA BRASSICICOLA; ARABIDOPSIS; ARABIDOPSIS THALIANA; BOTRYOTINIA FUCKELIANA; BOTRYTIS; ERWINIA; HYALOPERONOSPORA PARASITICA; PECTOBACTERIUM CAROTOVORUM; PERONOSPORA; PSEUDOMONAS; PSEUDOMONAS SYRINGAE; TEREBRANTIA;

EID: 0344153761     PISSN: 03781097     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1097(03)00759-6     Document Type: Article

References (34)

1. Martin F., Delaruelle A., Hilberty J.L. An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol. Res. 94:1990;1059-1064.
2. Plassard C.S., Mousain D.G., Salsac L.E. Estimation of mycelial growth of basidiomycetes by means of chitin determination. Phytochemistry. 21:1982;345-348.
3. Balesdent M.H., Desthieux I., Gall C., Robin P., Rouxel T. Quantification of Leptosphaeria-maculans growth in cotyledons of Brassica-napus using ELISA. J. Phytopathol. 143:1995;65-73.
4. Harrison J.G., Barker H., Lowe R., Rees E.A. Estimation of amounts of Phytophthora-infestans mycelium in leaf tissue by enzyme-linked-immunosorbent- assay. Plant Pathol. 39:1990;274-277.
5. Kamoun S., van West P., Govers F. Quantification of late blight resistance of potato using transgenic Phytophthora infestans expressing beta-glucuronidase. Eur. J. Plant Pathol. 104:1998;521-525.
6. Oliver R.P., Farman M.L., Jones J.D.G., Hammond-Kosack K.E. Use of fungal transformants expressing beta-glucuronidase activity to detect infection and measure hyphal biomass in infected-plant tissues. Mol. Plant-Microbe Interact. 6:1993;521-525.
7. Thomma B.P.H.J., Tadesse Y.S.H., Jacobs M., Broekaert W.F. Disturbed correlation between fungal biomass and β-glucuronidase activity in infections of Arabidopsis thaliana with transgenic Alternaria brassicicola. Plant Sci. 148:1999;31-36.
8. Dewey, F.M. and Thornton, C.R. (1995) Fungal immunodiagnostics in plant agriculture. In: New Diagnostics in Crop Sciences (Skerrit, J.H. and Appels, R., Eds.), pp. 151-170. CAB Int., Cambridge University Press, Cambridge.
9. Miller S.A. Detecting propagules of plant pathogenic fungi. Adv. Bot. Res. 23:1996;73-102.
10. Mahe A., Grisvard J., Dron M. Fungal-specific and plant-specific gene markers to follow the bean anthracnose infection process and normalize a bean chitinase mRNA induction. Mol. Plant-Microbe Interact. 5:1992;242-248.
11. Thomma B.P.H.J., Eggermont K., Penninckx I.A.M.A., Mauch-Mani B., Vogelsang R., Cammue B.P.A., Broekaert W.F. Separate jasmonate-dependent and salicylate-dependent defense response pathways in Arabidopsis are essential for resistance to distinct microbial pathogens. Proc. Natl. Acad. Sci. USA. 95:1998;15107-15111.
12. Hu X., Nazar R.N., Robb J. Quantification of Verticillium biomass in wilt disease development. Phys. Mol. Plant Pathol. 42:1993;23-36.
13. Lamar R.T., Schoenike B., Vanden Wymelenberg A., Stewart P., Dietrich D.M., Cullen D. Quantitation of fungal mRNAs in complex substrates by reverse transcription PCR and its application to Phanerochaete chrysosporium-colonized soil. Appl. Environ. Microbiol. 61:1995;2122-2126.
14. Heid C.A., Stevens J., Livak K.J., Williams P.M. Real time quantitative PCR. Genome Res. 6:1996;986-994.
15. Mackay I.M., Arden K.E., Nitsche A. Real-time PCR in virology. Nucleic Acids Res. 30:2002;1292-1305.
16. Wittwer C.T., Ririe K.M., Andrew R.V., David D.A., Gundry R.A., Balis U.J. The LightCycler™: a microvolume multisample fluorimeter with rapid temperature control. BioTechniques. 22:1997;176-181.
17. Holland P.M., Abramson R.D., Watson R., Gelfand D.H. Detection of specific polymerase chain reaction product by utilizing the 5′-3′ exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA. 88:1991;7276-7280.
18. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat. Biotechnol. 14:1996;303-308.
19. Whitcombe D., Theaker J., Guy S.P., Brown T., Little S. Detection of PCR products using self-probing amplicons and fluorescence. Nat. Biotechnol. 17:1999;804-807.
20. Morrison T.B., Weis J.J., Wittwer C.T. Quantification of low-copy transcripts by continuous SYBR Green I monitoring during amplification. BioTechniques. 24:1998;954-962.
21. Ausubel F.M., Katagiri F., Mindrinos M., Glazebrook J. Use of Arabidopsis thaliana defense-related mutants to dissect the plant response to pathogens. Proc. Natl. Acad. Sci. USA. 92:1995;4189-4196.
22. Thomma B.P.H.J., Penninckx I.A.M.A., Broekaert W.F., Cammue B.P.A. The complexity of disease signaling in Arabidopsis. Curr. Opin. Immunol. 13:2001;63-68.
23. Bent A.F., Innes R.W., Ecker J.R., Staskawicz B.J. Disease development in ethylene-insensitive Arabidopsis thaliana infected with virulent and avirulent Pseudomonas and Xanthomonas pathogens. Mol. Plant-Microbe Interact. 5:1992;372-378.
24. Hoffman T., Schmidt J.S., Zheng X., Bent A.F. Isolation of ethylene-insensitive soybean mutants that are altered in pathogen susceptibility and gene-for-gene disease resistance. Plant Physiol. 119:1999;935-950.
25. Thomma B.P.H.J., Eggermont K., Tierens K.F.M.-J., Broekaert W.F. Requirement of functional EIN2 (ethylene insensitive 2) gene for efficient resistance of Arabidopsis thaliana to infection by Botrytis cinerea. Plant Physiol. 121:1999;1093-1101.
26. Schaad N.W., Frederick R.D. Real-time PCR and its application for rapid plant disease diagnostics. Can. J. Plant Pathol. 24:2002;250-258.
27. Parker J.E., Holub E.B., Frost L.N., Falk A., Gunn N.D., Daniels M.J. Characterization of eds1, a mutation in Arabidopsis suppressing resistance to Peronospora parasitica specified by several different RPP genes. Plant Cell. 8:1996;2033-2046.
28. Thomma B.P.H.J., Nelissen I., Eggermont K., Broekaert W.F. Deficiency in phytoalexin production causes enhanced susceptibility of Arabidopsis thaliana to the fungus Alternaria brassicicola. Plant J. 19:1999;163-171.
29. The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408, 796-815.
30. Glazebrook J. Genes controlling expression of defense responses in Arabidopsis-2001 status. Curr. Opin. Plant Biol. 4:2001;301-308.
31. Böhm J., Hahn A., Schubert R., Bahnweg G., Adler N., Nechwatal J., Oehlmann R., Osswald W. Real-time quantitative PCR: DNA determination in isolated spores of the mycorrhizal fungus Glomus mosseae and monitoring of Phytophora infestans and Phytophthora citricola in their respective host plants. Phytopathol. Z. 14:1999;409-416.
32. Schaad N.W., Berthier-Schaad Y., Sechler A., Knorr D. Detection of Clavibacter michiganensis subsp. sepedonicus in potato tubers by BIO-PCR and an automated real-time fluorescence detection system. Plant Dis. 83:1999;1095-1100.
33. Weller A., Elphinstone J.G., Smith N.C., Boonham N., Stead D.E. Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, fluorogenic PCR (TaqMan) assay. Appl. Environ. Microbiol. 66:2000;2853-2858.
34. Winton L.M., Stone J.K., Watrud L.S., Hansen E.M. Simultaneous one-tube quantification of host and pathogen DNA with real-time polymerase chain reaction. Phytopathology. 92:2002;112-116.