Effect of promoter methylation of the p16 gene on phosphorylation of retinoblastoma gene product and growth of hepatocellular carcinoma cells

Tumor Biology

Volumn 26, Issue 6, 2005, Pages 300-305

  Maeta, Yoshiko ^a   Shiota, Goshi ^a   Okano, Jun Ichi ^a   Murawaki, Yoshikazu ^a  

^a TOTTORI UNIVERSITY   (Japan)

Author keywords

Hepatocellular carcinoma; Methylation; Mutation; p16; Retinoblastoma gene

Indexed keywords

GENE PRODUCT; MESSENGER RNA; PROTEIN P16;

ARTICLE; CONTROLLED STUDY; DNA SEQUENCE; GENE MUTATION; GENETIC ANALYSIS; HOMOZYGOSITY; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; METHYLATION; PHOSPHORYLATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; RETINOBLASTOMA; UPREGULATION; WESTERN BLOTTING;

EID: 27744483159     PISSN: 10104283     EISSN: None     Source Type: Journal    
DOI: 10.1159/000089288     Document Type: Article

References (25)

1. Tannapfel A, Wittekind C: Genes involved in hepatocellular carcinoma: deregulation in cell cycling and apoptosis. Virchows Arch 2002;440:345-352.
2. Feitelson MA, Sun B, Tufan NLS, Liu J, Pan J, Lian Z: Genetic mechanisms of hepatocarcinogenesis. Oncogene 2002;21:2593-2604.
3. Serrano M, Hannon GJ, Beach DA: A new regulatory motif in cell-cycle control causing specific inhibition of cyclinD/CDK4. Nature 1993;366:704-707.
4. Weinberg RA: The retinoblastoma gene and cell growth control. Trends Biochem Sci 1990;15:199-202.
5. Gonzalgo ML, Jones PA: Mutagenic and epigenetic effects of DNA methylation. Mutat Res 1997;386:107-118.
6. Tannapfel A, Benicke M, Weinans L, Katalinic A, Uhlmann D, Hauss J, Wittekind C: INK4a-ARF alterations and p53 mutations in hepatocellular carcinomas. Oncogene 2001;20:7104-7109.
7. Herman JG: Hypermethylation of tumor suppressor genes in cancer. Semin Cancer Biol 2000;9:359-367.
8. Slagle BL, Zhou YZ, Birchmeier W, Scorsone KA: Deletion of the E-cadherin gene in hepatitis B virus-positive Chinese hepatocellular carcinonomas. Hepatology 1993;18:757-762.
9. Liew CT, Li HM, Lo KW, Leow CK, Chan JY, Hin LY, Lau WY, Lai PB, Lim BK, Huang J, Leung WT, Wu S, Lee JC: High frequency of p16INK4A gene alterations in hepatocellular carcinoma. Oncogene 1999;120:789-795.
10. INK4A gene in liver cancer. Int J Cancer 1996;67:176-180.
11. KIP2 genes in hepatocellular carcinoma. Int J Oncol 1998;12:583-588.
12. Biden K, Young J, Buttenshaw, Searle J, Grahama G, Xu D-B, Leggett B: Frequency of mutation and deletion of the tumor suppressor gene CDK2A(MTS1/p16) in hepatocellular carcinoma from an Australian population. Hepatology 1997;25:593-597.
13. Matsuda Y, Ichida T, Matsuzawa J, Sugimura K, Asakura H: p16 (INK4) is inactivated by extensive CpG methylation in human hepatocellular carcinoma. Gastroenterology 1999;116:394-400.
14. Jin M, Piao Z, Kim NG, Park C, Shin EC, Park JH, Jung HJ, Kim CG, Kim H: p16 is a major inactivation target in hepatocellular carcinoma. Cancer 2000;89:60-68.
15. Herman JG, Graff JR, Myohanen S, Nelkin BD, Baylin SB: Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands. Proc Natl Acad Sci USA 1996;93:9821-9826.
16. Serrano M, Lee HW, Chin L, Cordon-Cardo C, Beach D, Depinho RA: Role of the INK4a locus in tumor suppression and cell mortality. Cell 1996;85:27-35.
17. Baylin SB, Herman JG, Graff JR, Vertino PM, Issa JP: Alterations in DNA methylation: a fundamental aspect of neoplasia. Adv Cancer Res 1998;72:141-196.
18. Merlo A, Herman JG, Mao L, Lee DJ, Gabreilson E, Burger PC, Baylin SB, Sidransky D: 5′ CpG island methylation is associated with transcriptional silencing of the tumour suppressor p16/CDKN2/MTS1 in human cancers. Nat Med 1995;1:686-692.
19. Belinsky SA, Nikura KJ, Palimisano WA, Michels R, Saccomanno G, Gabrielson E, Baylin SB, Herman JG: Aberrant methylation of the p16 (INK4a) is an early event in lung cancer and a potential biomarker for early diagnosis. Proc Natl Acad Sci USA 1998;95:11891-11896.
20. Hsieh CJ, Klump B, Holzmann K, Borchard F, Gregor M, Porschen R: Hypermethylation of p16IN4a promoter in colectomy specimens of patients with long-standing and extensive ulcerative colitis. Cancer Res 1998;58:3942-3945.
21. Klump B, Hsieh CJ, Holzmann K, Gregor M, Porschen R: Hypermethylation of the CDKN2/p16 promoter during neoplastic progression in Barrett's esophagus. Gastroenterology 1998;115:1381-1386.
22. Wilentz RE, Geradts J, Maynard R, Offerhaus GJ, Kang M, Goggins M, Yeo CJ, Kern SE, Hruban RH: Inactivation of the p16 (INK4A) tumor-suppressor gene in pancreatic duct lesions: loss of intranuclear expression. Cancer Res 1998;58:4740-4744.
23. Kaneto H, Sasaki S, Yamamoto H, Itoh F, Toyota M, Suzuki H, Ozeki I, Iwata N, Ohmura T, Satoh T, Karino Y, Toyota J, Endo T, Omata M, Imai K: Detection of hypermethylation of the p16INK4A gene promoter in chronic hepatitis and cirrhosis associated with hepatitis B or C virus. Gut 2001;48:372-377.
24. Kanai Y, Ushijima S, Tsuda H, Sakamoto M, Hirohashi S: Aberrant DNA methylation precedes loss of heterozygosity on chromosome 16 in chronic hepatitis and liver cirrhosis. Cancer Lett 2000;148:73-80.
25. Ronacalli M, Bianchi P, Bruni B, Laghi L, Destro A, Gioia SD, Gennari L, Tommasini M, Malesci A, Coggi G: Methylation framework of cell cycle gene inhibitors in cirrhosis and associated with hepatocellular carcinoma. Hepatology 2002;36:427-432.