Additional vectors for PCR-based gene tagging in Saccharomyces cerevisiae and Schizosaccharomyces pombe using nourseothricin resistance

Yeast

Volumn 22, Issue 13, 2005, Pages 1061-1068

  Van Driessche, Benoit ^a   Tafforeau, Lionel ^a   Hentges, Pierre ^b   Carr, Antony M ^b   Vandenhaute, Jean ^a  

^a UNIVERSITY OF NAMUR   (Belgium)

^b UNIVERSITY OF SUSSEX   (United Kingdom)

Author keywords

Epitope tagging; Gene overexpression; Nourseothricin; Vector; Yeast

Indexed keywords

EPITOPE; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HEMAGGLUTININ; NOURSEOTHRICIN;

AMINO TERMINAL SEQUENCE; ANTIBIOTIC RESISTANCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CHROMOSOMAL LOCALIZATION; CONTROLLED STUDY; FUNGAL STRAIN; FUNGUS GROWTH; GENE CASSETTE; GENE LOCUS; GENE VECTOR; GENETIC ANALYSIS; GENETIC MANIPULATION; NONHUMAN; PLASMID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FAMILY; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE; YEAST;

ANTIFUNGAL AGENTS; DRUG RESISTANCE, FUNGAL; EPITOPES; GENE TARGETING; GENETIC MARKERS; GENETIC VECTORS; PLASMIDS; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES; STREPTOTHRICINS; TRANSFORMATION, GENETIC;

FUNGI; SACCHAROMYCES CEREVISIAE; SCHIZOSACCHAROMYCES POMBE;

EID: 27744435957     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.1293     Document Type: Article

References (27)

1. Bahler J, Wu JQ, Longtine MS, et al. 1998. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe. Yeast 14: 943-951.
2. Bamps S, Westerling T, Pihlak A, et al. 2004. Mcs2 and a novel CAK subunit Pmh1 associate with Skp1 in fission yeast. Biochem Biophys Res Commun 325: 1424-1432.
3. Baudin A, Ozier-Kalogeropoulos O, Denouel A, Lacroute F, Cullin C. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res 21: 3329-3330.
4. Butland G, Peregrin-Alvarez JM, Li J, et al. 2005. Interaction network containing conserved and essential protein complexes in Escherichia coli. Nature 433: 531-537.
5. DeMarini DJ, Carlin EM, Livi GP. 2001. Constitutive promoter modules for PCR-based gene modification in Saccharomyces cerevisiae. Yeast 18: 723-728.
6. Forsburg SL. 1993. Comparison of Schizosaccharomyces pombe expression systems. Nucleic Acids Res 21: 2955-2956.
7. Gadal O, Strauss D, Petfalski E, et al. 2002. Rlp7p is associated with 60S preribosomes, restricted to the granular component of the nucleolus, and required for pre-rRNA processing. J Cell Biol 157: 941-951.
8. Gavin AC, Bosche M, Krause R, et al. 2002. Functional organization of the yeast proteome by systematic analysis of protein complexes. Nature 415: 141-147.
9. Goffeau A, Barrell BG, Bussey H, et al. 1996. Life with 6000 genes. Science 274: 546, 563-567.
10. Goldstein AL, McCusker JH. 1999. Three new dominant drug resistance cassettes for gene disruption in Saccharomyces cerevisiae. Yeast 15: 1541-1553.
11. Hentges P, Van Driessche B, Tafforeau L, Vandenhaute J, Carr AM. 2005. Three novel antibiotic marker cassettes for gene disruption and marker switching in Schizosaccharomyces pombe. Yeast 22: 1011-1017 (this issue).
12. Ho Y, Gruhler A, Heilbut A, et al. 2002. Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry. Nature 415: 180-183.
13. Ito-Harashima S, McCusker JH. 2004. Positive and negative selection LYS5MX gene replacement cassettes for use in Saccharomyces cerevisiae. Yeast 21: 53-61.
14. Janke C, Magiera MM, Rathfelder N, et al. 2004. A versatile toolbox for PCR-based tagging of yeast genes: new fluorescent proteins, more markers and promoter substitution cassettes. Yeast 21: 947-962.
15. Knop M, Siegers K, Pereira G, et al. 1999. Epitope tagging of yeast genes using a PCR-based strategy: more tags and improved practical routines. Yeast 15: 963-972.
16. Krawchuk MD, Wahls WP. 1999. High-efficiency gene targeting in Schizosaccharomyces pombe using a modular, PCR-based approach with long tracts of flanking homology. Yeast 15: 1419-1427.
17. Krugel H, Fiedler G, Smith C, Baumberg S. 1993. Sequence and transcriptional analysis of the nourseothricin acetyltransferase-encoding gene nat1 from Streptomyces noursei. Gene 127: 127-131.
18. Longtine MS, McKenzie A III, Demarini DJ, et al. 1998. Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae. Yeast 14: 953-961.
19. Mateus C, Avery SV. 2000. Destabilized green fluorescent protein for monitoring dynamic changes in yeast gene expression with flow cytometry. Yeast 16: 1313-1323.
20. Schneider BL, Seufert W, Steiner B, Yang QH, Futcher AB. 1995. Use of polymerase chain reaction epitope tagging for protein tagging in Saccharomyces cerevisiae. Yeast 11: 1265-1274.
21. Sikorski RS, Hieter P. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics 122: 19-27.
22. Sterner DE, Lee JM, Hardin SE, Greenleaf AL. 1995. The yeast carboxyl-terminal repeat domain kinase CTDK-I is a divergent cyclin-cyclin-dependent kinase complex. Mol Cell Biol 15: 5716-5724.
23. Tasto JJ, Carnahan RH, McDonald WH, Gould KL. 2001. Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe. Yeast 18: 657-662.
24. Vorachek-Warren MK, McCusker JH. 2004. DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast 21: 163-171.
25. Wach A, Brachat A, Alberti-Segui C, Rebischung C, Philippsen P. 1997. Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast 13: 1065-1075.
26. Wach A, Brachat A, Pohlmann R, Philippsen P. 1994. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10: 1793-1808.
27. Wood V, Gwilliam R, Rajandream MA, et al. 2002. The genome sequence of Schizosaccharomyces pombe. Nature 415: 871-880.