Vectors and gene targeting modules for tandem affinity purification in Schizosaccharomyces pombe

Yeast

Volumn 18, Issue 7, 2001, Pages 657-662

  Tasto, Joseph J ^a   Carnahan, Robert H ^a   Hayes McDonald, W ^a   Gould, Kathleen L ^a  

^a VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cdc2; Cell cycle; Fission yeast; Protein purification; Schizosaccharomyces pombe; Vectors

Indexed keywords

CALMODULIN BINDING PROTEIN; CYCLIN DEPENDENT KINASE; DNA; IMMUNOGLOBULIN G; STAPHYLOCOCCUS PROTEIN A;

AMINO TERMINAL SEQUENCE; ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA VECTOR; GENE CASSETTE; GENE TARGETING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PURIFICATION; REGULATORY MECHANISM; SCHIZOSACCHAROMYCES POMBE; TANDEM REPEAT;

BLOTTING, WESTERN; CDC2 PROTEIN KINASE; DNA, FUNGAL; FUNGAL PROTEINS; GENETIC VECTORS; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; SCHIZOSACCHAROMYCES;

SCHIZOSACCHAROMYCES POMBE;

EID: 0034980873     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.713     Document Type: Article

References (16)

1. Heterologous modules for efficient and versatile PCR-based gene targeting in Schizosaccharomyces pombe
2. TATA box mutations in the Schizosaccharomyces pombe nmt1 promoter affect transcription efficiency but not the transcription start point or thiamine repressibility
3. 1 phase of the cell cycle by periodic stabilization and degradation of the p25rum1 CDK inhibitor
4. + protein kinase
5. 2/M progression and cytokinesis in Schizosaccharomyces pombe
6. A fission yeast B-type cyclin functioning early in the cell cycle
7. Cell cycle regulation of the Saccharomyces cerevisiae polo-like kinase cdc5p
8. Cyclin dependent kinases
9. Phosphorylation at Thr167 is required for Schizosaccharomyces pombe p34cdc2 function
10. nmtl of fission yeast. A highly transcribed gene completely repressed by thiamine
11. Thiamine-repressible expression vectors pREP and pRIP for fission yeast
12. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe
13. High efficiency transformation of Schizosaccharomyces pombe by electroporation
14. A generic protein purification method for protein complex characterization and proteome exploration
15. PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in S. cerevisiae
16. New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae