Genotoxicity of nicotine in mini-organ cultures of human upper aerodigestive tract epithelia

Toxicological Sciences

Volumn 88, Issue 1, 2005, Pages 134-141

  Sassen, Andrea W ^a   Richter, Elmar ^b   Semmler, Marzell P ^a   Harréus, Ulrich A ^b   Gamarra, Fernando ^b   Kleinsasser, Norbert H ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b UNIVERSITY OF MUNICH   (Germany)

Author keywords

Comet assay; Genotoxicity; Human epithelia; Mini organ cultures; Nicotine

Indexed keywords

METHYLNITRONITROSOGUANIDINE; NICOTINE;

ADULT; ANALYSIS OF VARIANCE; ARTICLE; CELL MIGRATION; CELL VIABILITY; CLINICAL ARTICLE; COMET ASSAY; CONCENTRATION RESPONSE; DNA DAMAGE; DNA REPAIR; GENOTOXICITY; HUMAN; HUMAN CELL; NOSE MUCOSA; ORGAN CULTURE;

ADULT; AGED; CELL SURVIVAL; COMET ASSAY; DNA; DNA DAMAGE; DNA REPAIR; DOSE-RESPONSE RELATIONSHIP, DRUG; HUMANS; MALE; METHYLNITRONITROSOGUANIDINE; MIDDLE AGED; MUTAGENS; NASAL MUCOSA; NICOTINE; ORGAN CULTURE TECHNIQUES; TURBINATES;

NICOTIANA TABACUM;

EID: 27644484041     PISSN: 10966080     EISSN: 10960929     Source Type: Journal    
DOI: 10.1093/toxsci/kfi297     Document Type: Article

References (54)

1. Arabi, M. (2004). Nicotinic infertility: Assessing DNA and plasma membrane integrity of human spermatozoa. Andrologia 36, 305-310.
2. Argentin, G., and Ciechetti, R. (2004). Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts. Toxicol. Sci. 79, 75-81.
3. Becker, N. (2001). Epidemiologic aspects of cancer prevention in Germany. J. Cancer Res. Clin. Oncol. 127, 9-19.
4. Boffetta, P., Agudo, A., Ahrens, W., Benhamou, E., Benhamou, S., Darby, S. C., Ferro, G., Fortes, C., Gonzalez, C. A., Jöckel, K.-H., et al. (1998). Multicenter case-control study of exposure to environmental tobacco smoke and lung cancer in Europe. J. Natl. Cancer Inst. 90, 1440-1450.
5. Chang, Y. C., Huang, F. M., Tai, K. W., Yang, L. C., and Chou, M. Y. (2002). Mechanisms of cytotoxicity of nicotine in human periodontal ligament fibroblast cultures in vitro. J. Periodontal Res. 37, 279-285.
6. Charest, M., Rossignol, G., and Castonguay, A. (1989). In vitro and in vivo modulation of the bioactivation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in hamster lung tissues. Chem. Biol. Interact. 71, 265-278.
7. Chen, Y. C., Shen, S. C., Lin, H. Y., Tsai, S. H., and Lee, T. J. (2004). Nicotine enhancement of lipopolysaccharide/ interferon-gamma-induced cytotoxicity with elevating nitric oxide production. Toxicol. Lett. 153, 191-200.
8. Cheng, Y., Li, H. L., Wang, H. F., Sun, H. F., Liu, Y. F., Peng, S. X., Liu, K. X., and Guo, Z. Y. (2003). Inhibition of nicotine-DNA adduct formation in mice by six dietary constituents. Food Chem. Toxicol. 41, 1045-1050.
9. Cooke, J. P., and Bitterman, H. (2004). Nicotine and angiogenesis: A new paradigm for tobacco-related diseases. Ann. Med. 36, 33-40.
10. Crowley-Weber, C. L., Dvorakova, K., Crowley, C., Bernstein, H., Bernstein, C., Garewal, H., and Payne, C. M. (2003). Nicotine increases oxidative stress, activates NF-kappaB and GRP78, induces apoptosis and sensitizes cells to genotoxic/xenobiotic stresses by a multiple stress inducer, deoxycholate: Relevance to colon carcinogenesis. Chem. Biol. Interact. 145, 53-66.
11. Dajas-Bailador, F., and Wonnacott, S. (2004). Nicotinic acetylcholine receptors and the regulation of neuronal signalling. Trends Pharmacol. Sci. 25, 317-324.
12. Doolittle, D. J., Winegar, R., Lee, C. K., Caldwell, W. S., Hayes, A. W., and deBethizy, J. D. (1995). The genotoxic potential of nicotine and its major metabolites. Mutat. Res. 344, 95-102.
13. Harris, J. E., Thun, M. J., Mondul, A. M., and Calle, E. E. (2004). Cigarette tar yields in relation to mortality from lung cancer in the cancer prevention study II prospective cohort, 1982-8. Br. Med. J. 328, 72-76.
14. Heeschen, C., Jang, J. J., Weis, M., Pathak, A., Kaji, S., Hu, R. S., Tsao, P. S., Johnson, F. L., and Cooke, J. P. (2001). Nicotine stimulates angiogenesis and promotes tumor growth and atherosclerosis. Nat. Med. 7, 835-841.
15. Hoffmann, D., Hoffmann, I., and El-Bayoumy, K. (2001). The less harmful cigarette: A controversial issue. A tribute to Ernst L. Wynder. Chem. Res. Toxicol. 14, 767-790.
16. Hoffmann, H., and Speit, G. (2005). Assessment of DNA damage in peripheral blood of heavy smokers with the comet assay and the micronucleus test. Mut. Res. 581, 105-114.
17. Hukkanen, J., Jacob, P., and Benowitz, N. L. (2005). Metabolism and disposition kinetics of nicotine. Pharmacol. Rev. 57, 79-115.
18. IARC (2004). Tobacco smoke and involuntary smoking. IARC monographs on the Evaluation of Carcinogenic Risks to Humans, vol. 83. International Agency for Research on Cancer, Lyon, France.
19. John, U., and Hanke, M. (2002). Tobacco smoking- and alcohol drinking-attributable cancer mortality in Germany. Eur. J. Cancer Prev. 11, 11-17.
20. Kleinsasser, N. H., Gamarra, F., Bergner, A., Wallner, B. C., Harréus, U. A., Juchhoff, J., Kastenbauer, E. R., and Huber, R. M. (2001). Genotoxicity of nitroso compounds and sodium dichromate in a model combining organ cultures of human nasal epithelia and the comet assay. ORL 63, 141-147.
21. Kleinsasser, N. H., Harréus, U. A., Wallner, B. C., Sassen, A. W., Gamarra, F., Bührlen, M. (2005b). Cytochrome P450 stability in a model of human nasal mini-organ cultures for genotoxicology studies, (in preparation).
22. Kleinsasser, N. H., Juchhoff, J., Wallner, B. C., Bergner, A., Harréus, U. A., GamarTa, F., Bührlen, M. B., Huber, R. M., and Rettenmeier, A. W. (2004). The use of mini-organ cultures of human upper aerodigestive tract epithelia in ecogenotoxicology. Mutat. Res. 561, 63-73.
23. Kleinsasser, N. H., Sassen, A. W., Semmler, M. P., Harréus, U. A., and Richter, E. (2005a). The tobacco alkaloid nicotine demonstrates genotoxicity in human tonsillar tissue and lymphocytes. Toxicol. Sci. 86, 309-317.
24. Kleinsasser, N. H., Wallner, B. C., Harréus, U. A., Zwickenpflug, W., and Richter, E. (2003). Genotoxic effects of myosmine in human lymphocytes and upper aerodigestive tract epithelial cells. Toxicology 192, 171-177.
25. Labaj, J., Slamenova, D., and Kosikova, B. (2003). Reduction of genotoxic effects of the carcinogen N-methyl-N′-nitro-N-nitrosoguanidine by dietary lignin in mammalian cells cultured in vitro. Nutr. Cancer 47, 95-103.
26. Lee, C. K., Fulp, C., Bombick, B. R., and Doolittle, D. J. (1996). Inhibition of mutagenicity of N-nitrosamines by tobacco smoke and its constituents. Mutat. Res. 367, 83-92.
27. Lee, E., Oh, E., Lee, J., Sul, D., and Lee, J. (2004). Use of the tail moment of the lymphocytes to evaluate DNA damage in human biomonitoring studies. Toxicol. Sci. 81, 121-132.
28. Lee, P. N., and Sanders, E. (2004). Does increased cigarette consumption nullify any reduction in lung cancer risk associated with low-tar filter cigarettes? Inhal. Toxicol. 16, 817-833.
29. Levi, F., Lucchini, F., Negri, E., Boyle, P., and La Vecchia, C. (2004). Cancer mortality in Europe, 1995-1999, and an overview of trends since 1960. Int. J. Cancer 110, 155-169.
30. Li, X. S., Wang, H. F., Shi, J. Y., Wang, X. Y., Liu, Y. F., Li, K., Lu, X. Y., Wang, J. J., Liu, K. X., and Guo, Z. Y. (1996). Genotoxicity study on nicotine and nicotine-derived nitrosamine by accelerator mass spectrometry. Radiocarbon 38, 347-353.
31. Murphy, S. E., and Heiblum, R. (1990). Effect of nicotine and tobacco-specific nitrosamines on the metabolism of N′-nitrosonornicotine and 4-methylnitrosamino)-1-(3-pyridyl)-1-butanone by rat oral tissue. Carcinogenesis 11, 1663-1666.
32. National Cancer Institute (2001). Risks associated with smoking cigarettes with low machine-measured yields of tar and nicotine (D. R. Shopland, D. M. Burns, N. L. Benowitz, and R. H. Amacher, Eds.), Smoking and Tobacco Control Monograph No. 13. U.S. Department of Health and Human Services, National Institutes of Health, National Cancer Institute, Bethesda, MD.
33. Olive, P. L., Durand, R. E., Le Riche, J., Olivotto, I. A., and Jackson, S. M. (1993). Gel electrophoresis ofindividual cells to quantify hypoxic fraction in human breast cancers. Cancer Res. 53, 733-736.
34. Pool-Zobel, B. L., Lotzmann, N., Knoll, M., Kuchenmeister, F., Lambertz, R., Leucht, U., Schröder, H.-G., and Schmezer, P. (1994). Detection of genotoxic effects in human gastric and nasal mucosa cells isolated from biopsy samples. Environ. Mol. Mutagen. 24, 23-45.
35. Richter, E., and Tricker, A. R. (1994). Nicotine inhibits the metabolic activation of the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone in rats. Carcinogenesis 15, 1061-1064.
36. Richter, E., and Tricker, A. R. (2002). Effect of nicotine, cotinine and phenethyl isothiocyanate on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) metabolism in the Syrian golden hamster. Toxicology 179, 95-103.
37. Riche, M., and Westphal, K. (1983). Studies on the induction of sister-chromatid exchanges in Chinese hamster ovary cells by various alkaloids. Mutat. Res. 124, 281-286.
38. Riche, M., Westphal, K., and Fortnagel, P. (1982). Mutagenicity testing, in bacterial test systems, of some constituents of tobacco. Mutat. Res. 101, 39-43.
39. Sassen, A., Semmler, M., and Kleinsasser, N. (2003). Genotoxicity of nicotine in human mucosa and lymphocytes. J. Cancer Res. Clin. Oncol. 129, 30.
40. Schneider, N. G., Olmstaed, R. E., Franzon, M. A., and Lunell, E. (2001). The nicotine inhaler: Clinical pharmacokinetics and comparison with other nicotine treatments. Clin. Pharmacokinet. 40, 661-684.
41. Schuller, H. M. (1994). Carbon dioxide potentiates the mitogenic effects of nicotine and its carcinogenic derivative, NNK, in normal and neoplastic neuroendocrine lung cells via stimulation of autocrine and protein kinase C-dependent mitogenic pathways. Neurotoxicology 15, 877-886.
42. Schuller, H. M., Castonguay, A., Orloff, M., and Rossignol, G. (1991). Modulation of the uptake and metabolism of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone by nicotine in hamster lung. Cancer Res. 51, 2009-2014.
43. Schulze, J., Schrader, E., Foth, H., Kahl, G. F., and Richter, E. (1998). Effect of nicotine or cotinine on metabolism of 4-methylnitrosamino-1-(3-pyridyl)-1-butanone (NNK) in isolated rat lung and liver. Naunyn Schmiedebergs Arch. Pharmacol. 357, 344-350.
44. Shigenaga, M. K., Trevor, A. J., and Castagnoli, N. (1987). Metabolism-dependent covalent binding of (S)-[5-3H]nicotine to liver and lung microsomal macromolecules. Drug Metab. Dispos. 16, 397-402.
45. Shin, V. Y., Wu, W. K. K., Ye, Y.-N., So, W. H. L., Koo, M. W. L., Liu, E. S. L., Luo, J.-C., and Cho, C.-H. (2004). Nicotine promotes gastric tumor growth and neovascularization by activating extracellular signal-regulated kinase and cyclooxygenase-2. Carcinogenesis 25, 2487-2495.
46. Steinsvåg, S. K., Strand, M., Berg, Ø., Miaguchi, M., and Olofsson, J. (1991). Human respiratory mucosa in a nonadhesive stationary organ culture system. Laryngoscope 101, 1323-1331.
47. Sun, H. F., He, L., Liu, Y. F., Liu, K. X., Lu, X. Y., Wang, J. J., Ma, H. J., and Li, K. (2000). Decay kinetics of nicotine/NNK-DNA adducts in vivo studied by accelerator mass spectrometry. NIM B 172, 915-919.
48. 14C]nicotine in the mouse: Retention in the bronchi, melanin-containing tissues and urinary bladder wall. Toxicology 10, 207-220.
49. Teissier, E., Fennrich, S., Strazielle, N., Daval, J. L., Ray, D., Schlosshauer, B., and Ghersi-Egea, J. F. (1998). Drug metabolism in in vitro organotypic and cellular models of mammalian central nervous system: Activities of membrane-bound epoxide hydrolase and NADPH-cytochrome P-450 (c) reductase. Neurotoxicology 19, 347-355.
50. Teneggi, V., Squassante, L., Iavarone, L., Milleri, S., Bye, A., Gomeni, R. (2002). Correlation and predictive performances of saliva and plasma nicotine concentration on tobacco withdrawel-induced craving. Br. J. Clin. Pharmacol. 54, 407-414.
51. Tice, R. R., Agurell, E., Anderson, D., Burlinson, B., Hartmann, A., Kobayashi, H., Miyamae, Y., Rojas, E., Ryu, J. C., and Sasaki, Y. F. (2000). Single cell gel/comet assay: Guidelines for in vitro and in vivo genetic toxicology testing. Environ. Mol. Mutagen. 35, 206-221.
52. Trivedi, A. H., Dave, B. J., and Adhvaryu, S. G. (1990). Assessment of genotoxicity of nicotine employing in vitro mammalian test system. Cancer Lett. 54, 89-94.
53. Wetscher, G. J., Hinder, P. R., Bagchi, D., Perdikis, G., Redmont, E. J., Adrian, T. E., Hinder, R. A. (1995). Free radical scavengers prevent reflux esophagitis in rats. Dig. Dis. Sci. 40, 1292-1296.
54. Wu, X. H., Wang, H. F., Liu, Y. F., Lu, X. Y., Wang, J. J., and Li, K. (1997). Histone adduction with nicotine: A bio-AMS study. Radiocarbon 39, 293-297.