Human galectin-2: Expression profiling by RT-PCR/immunohistochemistry and its introduction as a histochemical tool for ligand localization

Histology and Histopathology

Volumn 20, Issue 4, 2005, Pages 1191-1208

  Saal, I ^a   Lensch, M ^b   Lohr, M ^b   Manning, J C ^b   Decaestecker, C ^c   Andre S ^b   Kiss, R ^c   Salmon, Isabelle ^a   Gabius, H J ^b  

^a ERASME HOSPITAL   (Belgium)

^b UNIVERSITY OF MUNICH   (Germany)

^c FREE UNIVERSITY OF BRUSSELS   (Belgium)

Author keywords

Biliary tract; Epithelium; Galectin; Glycoprotein; Inflammation

Indexed keywords

GALECTIN 1; GALECTIN 2; GALECTIN 3; GALECTIN 4; GALECTIN 7; GALECTIN 8; LECTIN; MESSENGER RNA; POLYCLONAL ANTIBODY;

ARTICLE; AUTOPSY; BIOTINYLATION; BLADDER CARCINOMA; CELL NUCLEUS; COLON ADENOCARCINOMA; CONTROLLED STUDY; CYTOPLASM; GENE EXPRESSION PROFILING; HISTOPATHOLOGY; HUMAN; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; INFLAMMATION; KIDNEY TUMOR; LUNG ADENOCARCINOMA; MAJOR CLINICAL STUDY; PROGNOSIS; PROTEIN EXPRESSION; PROTEIN FAMILY; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA FINGERPRINTING; STOMACH ADENOCARCINOMA; THYROID PAPILLARY CARCINOMA;

ANTIBODIES; BIOLOGICAL MARKERS; GALECTIN 1; GALECTIN 2; GALLBLADDER; GENE EXPRESSION PROFILING; HUMANS; IMMUNOHISTOCHEMISTRY; LECTINS; LIGANDS; ORGAN SPECIFICITY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

EID: 27144539890     PISSN: 02133911     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (97)

1. Ahmad N., Gabius H.-J., Kaltner H., André S., Kuwabara I., Liu F.-T., Oscarson S., Norberg T. and Brewer C.F. (2002). Thermodynamic binding studies of cell surface carbohydrate epitopes to galectins-1, -3, and -7: evidence for differential binding specificities. Can. J. Chem. 80, 1096-1104.
2. André S., Kojima S., Yamazaki N., Fink C., Kaltner H., Kayser K. and Gabius H.-J. (1999). Galectins-1 and -3 and their ligands in tumor biology. J. Cancer Res. Clin. Oncol. 125, 461-474.
3. André S., Pieters R.J., Vrasidas I., Kaltner H., Kuwabara I., Liu F.-T., Liskamp R.M.J. and Gabius H.-J. (2001). Wedgelike glycodendrimers as inhibitors of binding of mammalian galectins to glycoproteins, lactose maxiclusters, and cell surface glycoconjugates. ChemBioChem 2, 822-830.
4. André S., Liu B., Gabius H.-J. and Roy R. (2003). First demonstration of differential inhibition of lectin binding by synthetic tri- and tetravalent glycoclusters from cross-coupling of rigidified 2-propynyl lactoside. Org. Biomol. Chem. 1, 3909-3916.
5. André S., Kaltner H., Furuike T., Nishimura S.-I. and Gabius H.-J. (2004a). Persubstituted cyclodextrin-based glycoclusters as inhibitors of protein-carbohydrate recognition using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets. Bioconjugate Chem. 15, 87-98.
6. André S., Unverzagt C., Kojima S., Frank M., Seifert J., Fink C., Kayser K., von der Lieth C.-W. and Gabius H.-J. (2004b). Determination of modulation of ligand properties of synthetic complex-type biantennary N-glycans by introduction of bisecting GIcNAc in silico, in vitro and in vivo. Eur. J. Biochem. 271, 118-134.
7. André S., Kaltner H., Lensch M., Russwurm R., Siebert H.-C., Fallsehr C., Tajkhorshid E., Heck A.J.R., von Knebel-Döberitz M., Gabius H.-J. and Kopitz J. (2005a). Determination of structural and functional overlap/divergence of five proto-type galectins by analysis of the growth-regulatory interaction with ganglioside GM1 in silico and in vitro on human neuroblastoma cells. Int. J. Cancer 114, 46-57.
8. André S., Arnusch C.J., Kuwabara I., Russwurm R., Kaltner H., Gabius H.-J. and Pieters R.J. (2005b). Identification of peptide ligands for malignancy- and growth-regulating galectins using random phage-display and designed combinatorial peptide libraries. Bioorg. Med. Chem. 13, 563-573.
9. André S., Kojima S., Prahl I., Lensch M., Unverzagt C. and Gabius H.-J. (2005c). Introduction of extended LEC14-type branching into core-fucosylated biantennary N-glycan. FEBS J. 272, 1986-1998.
10. Angata T. and Brinkman-Van der Linden E.C.M. (2002). I-type lectins. Biochim. Biophys. Acta 1572, 294-316.
11. Arnusch C.J., André S., Valentini P., Lensch M., Russwurm R., Siebert H.-C., Fischer M.J.E., Gabius H.-J. and Pieters R.J. (2004). Interference of the galactose-dependent binding of lectins by novel pentapeptide ligands. Bioorg. Med. Chem. Lett. 14, 1437-1440.
12. Brockhausen I. and Schachter H. (1997). Glycosyltransferases involved in N- and O-glycan biosynthesis. In: Glycosciences: Status and perspectives. Gabius H.-J. and Gabius S. (eds). Chapman & Hall, Weinheim-London. pp 79-113.
13. Brockhausen I., Schutzbach J. and Kuhns W. (1998). Glycoproteins and their relationship to human disease. Acta Anat. 161, 36-78.
14. Camby I., Belot N., Rorive S., Lefranc F., Maurage C.-A., Lahm H., Kaltner H., Hadari Y.R., Ruchoux M.-M., Brotchi J., Zick Y., Salmon I., Gabius H.-J. and Kiss R. (2001). Galectins are differentially expressed in supratentorial pilocytic astrocytomas, astrocytomas, anaplastic astrocytomas and glioblastomas, and significantly modulate tumor astrocyte migration. Brain Pathol. 11, 12-26.
15. Caselitz J. (1987). Lectins and blood group substances as tumor markers. Curr. Top. Pathol. 77, 245-278.
16. Cooper D.N.W. (2002). Galectinomics: finding themes in complexity. Biochim. Biophys. Acta 1572, 209-231.
17. Crocker P.R. (2004). CD33-related siglecs in the immune system. Trends Glycosci. Glycotechnol. 16, 357-370.
18. Cummings F.D. (1997). Lectins as tools for glycoconjugate purification and characterization. In: Glycosciences: Status and perspectives. Gabius H.-J. and Gabius S. (eds). Chapman & Hall. Weinheim-London. pp 191-199.
19. Dahms N.M. and Hancock M.K. (2002). P-type lectins. Biochim. Biophys. Acta 1572, 317-340.
20. Danguy A., Akif F., Pajak B. and Gabius H.-J. (1994). Contribution of carbohydrate histochemistry to glycobiology. Histol. Histopathol. 9, 155-171.
21. Danguy A., Rorive S., Decaestecker C., Bronckart Y., Kaltner H., Hadari Y.R., Goren R., Zick Y., Petein M., Salmon I., Gabius H.-J. and Kiss R. (2001). Immunohistochemical profile of galectin-8 expression in benign and malignant tumors of epithelial, mesenchymatous and adipous origins, and of the nervous system. Histol. Histopathol. 16, 861-868.
22. Danguy A., Camby I. and Kiss R. (2002). Galectins and cancer. Biochim. Biophys. Acta 1572, 285-293.
23. Delorge S., Saussez S., Pelc P., Devroede B., Marchant H., Burchert M., Zeng F.-Y., Danguy A., Salmon I., Gabius H.-J., Kiss R. and Hassid S. (2000). Correlation of galectin-3/galectin-3-binding sites with low differentiation status in head and neck squamous cell carcinomas. Otolaryngol. Head Neck Surg. 122, 834-841.
24. François C., van Velthoven R., De Lathouwer O., Moreno C., Peltier A., Kaltner H., Salmon I., Gabius H.-J., Danguy A., Decaestecker C. and Kiss R. (1999). Galectin-1 and galectin-3 binding pattern expression in renal cell carcinomas. Am. J. Clin. Pathol. 112, 194-203.
25. Gabius H.-J. (1990). Influence of type of linkage and spacer on the interaction of β-galactoside-binding proteins with immobilized affinity ligands. Anal. Biochem. 189, 91-94.
26. Gabius H.-J. (1997a). Animal lectins. Eur. J. Biochem. 243, 543-576.
27. Gabius H.-J. (1997b). Concepts of tumor lectinology. Cancer Invest. 15, 454-464.
28. Gabius H.-J. (1998). The how and why of protein-carbohydrate interaction: a primer to the theoretical concept and a guide to application in drug design. Pharmaceut. Res. 15, 23-30.
29. Gabius H.-J. (2000). Biological information transfer beyond the genetic code: the sugar code. Naturwissenschaften 87, 108-121.
30. Gabius H.-J. (2001). Glycohistochemistry: the why and how of detection and localization of endogenous lectins. Anat. Histol. Embryol. 30, 3-31.
31. Gabius H.-J., Brehler R., Schauer A. and Cramer F. (1986a). Localization of endogenous lectins in normal human breast, benign breast lesions and mammary carcinomas. Virchows Arch. [Cell. Pathol.] 52, 107-115.
32. Gabius H.-J., Engelhardt R., Rehm S., Barondes S.H. and Cramer F. (1986b). Presence and relative distribution of three endogenous β-galactoside-specific lectins in different tumor types of rat. Cancer J. 1, 19-22.
33. Gabius H.-J., Wosgien B., Hendrys M. and Bardosi A. (1991). Lectin localization in human nerve by biochemically defined lectin-binding glycoproteins, neoglycoprotein and lectin-specific antibody. Histochemistry 95, 269-277.
34. Gabius H.-J., André S., Kaltner H. and Siebert H.-C. (2002). The sugar code: functional lectinomics. Biochim. Biophys. Acta 1572, 165-177.
35. Gabius H.-J., Siebert H.-C., André S., Jiménez-Barbero J. and Rüdiger H. (2004). Chemical biology of the sugar code. ChemBioChem 5, 740-764.
36. Gitt M.A. and Barondes S.H. (1986). Evidence that a human soluble β-galactoside-binding lectin is encoded by a family of genes. Proc. Natl. Acad. Sci. USA 83, 7603-7607.
37. Gitt M.A. and Barondes S.H. (1991). Genomic sequence and organization of two members of a human lectin gene family. Biochemistry 30, 82-89.
38. Gitt M.A., Massa S.M., Leffler H. and Barondes S.H. (1992). Isolation and expression of a gene encoding L-14-II, a new human soluble lactose-binding lectin. J. Biol. Chem. 267, 10601-10606.
39. Haltiwanger R.S. and Lowe J.B. (2004). Role of glycosylation in development. Annu. Rev. Biochem. 73, 491-537.
40. Hancq S., Salmon I., Brotchi J., Gabius H.-J., Heizmann C. W., Kiss R. and Decaestecker C. (2004). Detection of S100B, S100A6 and galectin-3 ligands in meningiomas as markers of aggressiveness. Int. J. Oncol. 25, 1233-1240.
41. He L., André S., Siebert H.-C., Helmholz H., Niemeyer B. and Gabius H.-J. (2003). Detection of ligand- and solvent-induced shape alterations of cell-growth-regulatory human lectin galectin-1 in solution by small angle neutron and x-ray scattering. Biophys. J. 85, 511-524.
42. Hirabayashi J. (2004). On the origin of glycome and saccharide recognition. Trends Glycosci. Glycotechnol. 16, 63-85.
43. Hirabayashi J., Hashidate T., Arata Y., Nishi N., Nakamura T., Hirashima M., Urashima T., Oka T., Futai M., Müller W.E.G., Yagi F. and Kasai K.-i. (2002). Oligosaccharide specificity of galectins: a search by frontal affinity chromatography. Biochim. Biophys. Acta 1572, 232-254.
44. Houzelstein D., Gonçalves I.R., Fadden A.J., Sidhu S.S., Cooper D.N.W., Drickamer K., Leffler H. and Poirier F. (2004). Phylogenetic analysis of the vertebrate galectin family. Mol. Biol. Evol. 21, 1177-1187.
45. Kaltner H. and Stierstorfer B. (1998). Animal lectins as cell adhesion molecules. Acta Anat. 161, 162-179.
46. Kaltner H., Lips K.S., Reuter G., Lippert S., Sinowatz F. and Gabius H.-J. (1997). Quantitation and histochemical localization of galectin-1 and galectin-1-reactive glycoconjugates in fetal development of bovine organs. Histol. Histopathol. 12, 945-960.
47. Kaltner H., Seyrek K., Heck A., Sinowatz F. and Gabius H.-J. (2002). Galectin-1 and galectin-3 in fetal development of bovine respiratory and digestive tracts. Cell Tissue Res. 307, 35-46.
48. Kanazawa N., Tashiro K. and Miyachi Y. (2004). Signaling and immune regulatory role of the dendritic cell immunoreceptor (DCIR) family lectins: DCIR, DCAR, dectin-2 and BDCA-2. Immunobiology 209, 179-190.
49. Kayser K., Höft D., Hufnagl P., Caselitz J., Zick Y., André S., Kaltner H. and Gabius H.-J. (2003). Combined analysis of tumor growth pattern and expression of endogenous lectins as a prognostic tool in primary testicular cancer and its lung metastases. Histol. Histopathol. 18, 771-779.
50. Kopitz J., André S., von Reitzenstein C., Versluis K., Kaltner H., Pieters R.J., Wasano K., Kuwabara I., Liu F.-T., Cantz M., Heck A.J.R. and Gabius H.-J. (2003). Homodimeric galectin-7 (p53-induced gene 1) is a negative growth regulator for human neuroblastoma cells. Oncogene 22, 6277-6288.
51. Kuchler S., Zanetta J.-P., Vincendon G. and Gabius H.-J. (1990). Detection of binding sites for biotinylated neoglycoproteins and heparin (endogenous lectins) during cerebellar ontogenesis in the rat. Eur. J. Cell Biol. 52, 87-97.
52. Laine R.A. (1997). The information-storing potential of the sugar code. In: Glycosciences: Status and perspectives. Gabius H.-J. and Gabius S. (eds). Chapman & Hall. Weinheim-London. pp 1-14.
53. Lahm H., André S., Höflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E. and Gabius H.-J. (2001). Comprehensive galectin fingerprinting in a panel of 61 human tumor cell lines by RT-PCR and its implications for diagnostic and therapeutic procedures. J. Cancer Res. Clin. Oncol. 127, 375-386.
54. Lahm H., André S., Höflich A., Fischer J.R., Sordat B., Kaltner H., Wolf E. and Gabius H.-J. (2003). Molecular biological fingerprinting of human lectin expression by RT-PCR. Methods Enzymol. 362, 287-297.
55. Lahm H., André S., Höflich A., Kaltner H., Siebert H.-C., Sordat B., von der Lieth C.-W., Wolf E. and Gabius H.-J. (2004). Tumor galectinology: insights into the complex network of a family of endogenous lectins. Glycoconjugate J. 20, 227-238.
56. Lefranc F., Mijatovic T., Decaestecker C., Kaltner H., André S., Brotchi J., Salmon I., Gabius H.-J. and Kiss R. (2005). Monitoring the expression profiles of integrins and adhesion/growth-regulatory galectins in adamantinomatous craniopharyngiomas: their ability to regulate tumor adhesiveness to surrounding tissue and their contribution to prognosis. Neurosurgery 56, 763-776.
57. Liu F.-T. (2004). Double identity: galectins may not function as lectins inside the cell. Trends. Glycosci. Glycotechnol. 16, 255-264.
58. Liu F.-T., Patterson R.J. and Wang J.L. (2002). Intracellular functions of galectins. Biochim. Biophys. Acta 1572, 263-273.
59. Lobsanov Y.D., Gitt M.A., Leffler H., Barondes S.H. and Rini J.M. (1993). X-ray crystal structure of the human dimeric S-lac lectin, L-14-II, in complex with lactose at 2.9 Å resolution. J. Biol. Chem. 268, 27034-27038.
60. López-Lucendo M.F., Solís D., André S., Hirabayashi J., Kasai K.-i., Kaltner H., Gabius H.-J. and Romero A. (2004). Growth-regulatory human galectin-1: crystallographic characterization of the structural changes induced by single-site mutations and their impact on the thermodynamics of ligand binding. J. Mol. Biol. 343, 957-970.
61. Manning J.C., Seyrek K., Kaltner H., André S., Sinowatz F. and Gabius H.-J. (2004). Glycomic profiling of developmental changes in bovine testis by lectin histochemistry and further analysis of the most prominent alteration on the level of the glycoproteome by lectin blotting and lectin affinity chromatography. Histol. Histopathol. 19, 1043-1060.
62. Marshall A.S.J. and Gordon S. (2004). C-type lectins on the macrophage cell surface - recent findings. Eur. J. Immunol. 34, 18-24.
63. McGreal E.P., Miller J.L. and Gordon S. (2005). Ligand recognition by antigen-presenting cell C-type lectin receptors. Curr. Opin. Immunol. 17, 18-24.
64. Nagy N., Decaestecker C., Dong X., Kaltner H., Schüring M.-P., Rocmans P., Danguy A., Gabius H.-J., Kiss R. and Salmon I. (2000). Characterization of ligands for galectins, natural galactoside-binding immunoglobulin G subtractions and sarcolectin and also of the expression of calcyclin in thyroid lesions. Histol. Histopathol. 15, 503-513.
65. Nagy N., Bronckart Y., Camby I., Legendre H., Lahm H., Kaltner H., Hadari Y., Van Ham P., Yeaton P., Pector J.C., Zick Y., Salmon I., Danguy A., Kiss R. and Gabius H.-J. (2002). Galectin-8 expression decreases in cancer compared with normal and dysplastic human colon tissue and acts significantly on human colon cancer cell migration as a suppressor. Gut 50, 392-401.
66. Nagy N., Legendre H., Engels O., André S., Kaltner H., Wasano K., Zick Y., Pector J.C., Decaestecker C., Gabius H.-J., Salmon I. and Kiss R. (2003). Refined prognostic evaluation in colon carcinoma using immunohistochemical galectin fingerprinting. Cancer 97, 1849-1858.
67. Nomura K. (2004). Sugar chains in cell adhesion and cell division: comparative glycomics throwing light on glycobiology. Trends Glycosci. Glycotechnol. 16, 125-134.
68. Oka T., Murakami S., Arata Y., Hirabayashi J., Kasai K.-i., Wada Y. and Futai M. (1999). Identification and cloning of rat galectin-2: expression is predominantly in epithelial cells of the stomach. Arch. Biochem. Biophys. 361, 195-201.
69. Ozaki K., Ohnishi Y., Iida A., Sekine A., Yamada R., Tsunoda T., Sato H., Sato H., Hori M., Nakamura Y. and Tanaka T. (2002). Functional SNPs in the lymphotoxin-α gene that are associated with susceptibility to myocardial infarction. Nature Genet. 32, 650-654.
70. Ozaki K., Inoue K., Sato H., Iida A., Ohnishi Y., Sekine A., Sato H., Odashiro K., Nobuyoshi M., Hori M., Nakamura Y. and Tanaka T. (2004). Functional variation in LGALS2 confers risk of myocardial infarction and regulates lymphotoxin-α secretion in vitro. Nature 429, 72-75.
71. Paron I., Scaloni A., Pines A., Bachi A., Liu F.-T., Puppin C., Pandolfi M., Ledda L., Di Loreto C., Damante G. and Tell G. (2003). Nuclear localization of galectin-3 in transformed thyroid cells: a role in transcriptional regulation. Biochem. Biophys. Res. Commun. 302, 545-553.
72. Pavelka M. (1997). Topology of glycosylation - a histochemist's view. In: Glycosciences: Status and perspectives. Gabius H.-J. and Gabius S. (eds). Chapman & Hall. Weinheim-London. pp 115-120.
73. Paz A., Haklai R., Elad-Sfadia G., Ballan E. and Kloog Y. (2001). Galectin-1 binds oncogenic H-Ras to mediate Ras membrane anchorage and cell transformation. Oncogene 20, 7486-7493.
74. Plzák J., Betka J., Smetana K.Jr., Chovanec M., Kaltner H., André S., Kodet R. and Gabius H.-J. (2004). Galectin-3: an emerging prognostic indicator in advanced head and neck carcinoma. Eur. J. Cancer 40, 2324-2330.
75. Purkrábková T., Smetana K. Jr., Dvonánková B., Holiková Z., Böck C., Lensch M., André S., Pytlík R., Liu F.-T., Klíma J., Smetana K., Motlík J. and Gabius H.-J. (2003). New aspects of galectin functionality in nuclei of cultured bone marrow stromal and epidermal cells: biotinylated galectins as tool to detect specific binding sites. Biol. Cell 95, 535-545.
76. Reuter G. and Gabius H.-J. (1999). Eukaryotic glycosylation: whim of nature or multipurpose tool? Cell. Mol. Life Sci. 55, 368-422.
77. Rotblat B., Niv H., André S., Kaltner H., Gabius H.-J. and Kloog Y. (2004). Galectin-1(L11A) predicted from a computed galectin-1 farnesyl-binding pocket selectively inhibits Ras-GTP. Cancer Res. 64, 3112-3118.
78. Rüdiger H. and Gabius H.-J. (2001). Plant lectins. Glycoconjugate J. 18, 589-613.
79. Saussez S., Nonclercq D., Laurent G., Wattiez R., André S., Kaltner H., Gabius H.-J., Kiss R. and Toubeau G. (2005). Toward functional glycomics by localization of tissue lectins: immunohistochemical galectin fingerprinting during diethylstilbestrol-induced kidney tumorigenesis in male Syrian hamster. Histochem. Cell Biol. 123, 29-41.
80. Schreyer S.A., Vick C.M. and LeBoeuf R.C. (2002). Loss of lymphotoxin-α but not tumor necrosis factor-α reduces atherosclerosis in mice. J. Biol. Chem. 277, 12364-12368.
81. Schwarz G., Remmelink M., Decaestecker C., Gielen I., Budel V., Burchert M., Darro F., Danguy A., Gabius H.-J., Salmon I. and Kiss R. (1999). Galectin fingerprinting in tumor diagnosis. Differential expression of galectin-3 and galectin-3-binding sites, but not of galectin-1, in benign versus malignant uterine smooth muscle tumors. Am. J. Clin. Pathol. 111, 623-631.
82. Sharma U.C., Pokharel S., van Brakel T.J., van Berlo J.H., Cleutjens J.P.M., Schroen B., André S., Crijns H.J.G.M., Gabius H.-J., Maessen J. and Pinto Y.M. (2004). Galectin-3 marks activated macrophages in failure-prone hypertrophied hearts and contributes to cardiac dysfunction. Circulation 110, 3121-3128.
83. Sharon N. and Lis H. (1997). Glycoproteins: structure and function. In: Glycosciences: Status and perspectives. Gabius H.-J. and Gabius S. (eds). Chapman & Hall, Weinheim-London. pp 133-162.
84. Sheikholeslam-Zadeh R., Decaestecker C., Delbrouck C., Danguy A., Salmon I., Zick Y., Kaltner H., Hassid S., Gabius H.-J., Kiss R. and Choufani G. (2001). The levels of expression of galectin-3, but not of galectins-1 and -8, correlate with apoptosis in human cholesteatoma. Laryngoscope 111, 1042-1047.
85. Shimura T., Takenaka Y., Tsutsumi S., Hogan V., Kikuchi A. and Raz A. (2004). Galectin-3, a novel binding partner of β-catenin. Cancer Res. 64, 6363-6367.
86. 1 versus bacterial toxins. Biochemistry 42, 14762-14773.
87. Siebert H.-C., Jiménez-Barbero J., André S., Kaltner H. and Gabius H.-J. (2003b). Describing topology of bound ligands by transferred nuclear Overhauser effect spectroscopy and molecular modeling. Methods Enzymol. 362, 417-434.
88. Solmi R., De Sanctis P., Zucchini C., Ugolini G., Rosati G., Del Governatore M., Coppola D., Yeatman T.J., Lenzi L., Caira A., Zanotti S., Taffurelli M., Carinci P., Valvassori L. and Strippoli P. (2004). Search for epithelial-specific mRNAs in peripheral blood of patients with colon cancer by RT-PCR. Int. J. Oncol. 25, 1049-1056.
89. Spicer S.S. and Schulte B.A. (1992). Diversity of cell glycoconjugates shown histochemically: a perspective. J. Histochem. Cytochem. 40, 1-38.
90. Spiro R.G. (2002). Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology 12, 43R-56R.
91. Sturm A., Lensch M., André S., Kaltner H., Wiedenmann B., Rosewicz S., Dignass A.U. and Gabius H.-J. (2004). Human galectin-2: novel inducer of T cell apoptosis with distinct profile of caspase activation. J. Immunol. 173, 3825-3837.
92. Villalobo A., Nogales-Gonzáles A. and Gabius H.-J. (2005). A guide to signaling pathways connecting protein-glycan interaction with the emerging versatile effector functionality of mammalian lectins. Trends Glycosci. Glycotechnol. (in press).
93. Wollina U., Schreiber G., Görnig M., Feldrappe S., Burchert M. and Gabius H.-J. (1999). Sertoli cell expression of galectins-1 and -3 and accessible binding sites in normal human testis and Sertoli cell only-syndrome. Histol. Histopathol. 14, 779-784.
94. Wollina U., Graefe T., Feldrappe S., André S., Wasano K., Kaltner H., Zick Y. and Gabius H.-J. (2002). Galectin fingerprinting by immuno- and lectin histochemistry in cutaneous lymphoma. J. Cancer Res. Clin. Oncol. 128, 103-110.
95. Wu A.M., Wu J.H., Tsai M.-S., Kaltner H. and Gabius H.-J. (2001). Carbohydrate specificity of a galectin from chicken liver (CG-16). Biochem. J. 358, 529-538.
96. Wu A.M., Wu J.H., Tsai M.-S., Liu J.-H., André S., Wasano K., Kaltner H. and Gabius H.-J. (2002). Fine-specificity of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N). Biochem. J. 367, 653-664.
97. Wu A.M., Wu J.H., Liu J.-H., Singh T., André S., Kaltner H. and Gabius H.-J. (2004). Effects of polyvalency of glycotopes and natural modifications of human blood group ABH/Lewis sugars at the Galβ1-terminated core saccharides on the binding of domain-I of recombinant tandem-repeat-type galectin-4 from rat gastrointestinal tract (G4-N). Biochimie 86, 317-326.