Structural biology: Tryptophan 7-halogenase (PrnA) structure suggests a mechanism for regioselective chlorination

Science

Volumn 309, Issue 5744, 2005, Pages 2216-2219

  Dong, Changjiang ^a   Flecks, Silvana ^b   Unversucht, Susanne ^b   Haupt, Caroline ^b   Van Pée, Karl Heinz ^b   Naismith, James H ^a  

^a UNIVERSITY OF ST ANDREWS   (United Kingdom)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; BIOSYNTHESIS; CRYSTAL STRUCTURE; DECOMPOSITION; ENZYMES;

REGIOSELECTIVITY; TRYPTOPHAN;

CHLORINATION;

CHLORIDE ION; CRYPTOPHYCIN A; ENZYME; FLAVIN DEPENDENT MONOOXYGENASE; FLAVINE ADENINE NUCLEOTIDE; HYDROGEN; HYPOCHLOROUS ACID; NATURAL PRODUCT; OXYGEN; PEROXY RADICAL; PEROXYFLAVIN; QUERCETIN; REDUCED FLAVIN ADENINE NUCLEOTIDE; TRYPTOPHAN; TRYPTOPHAN 7 HALOGENASE; UNCLASSIFIED DRUG; UNSPECIFIC MONOOXYGENASE; VANCOMYCIN; 1,5 DIHYDRO FAD; 1,5-DIHYDRO-FAD; 5 METHYLINDOLE; 5-METHYLINDOLE; 7 CHLOROTRYPTOPHAN; 7-CHLOROTRYPTOPHAN; CHLORIDE; DRUG DERIVATIVE; INDOLE DERIVATIVE; OXIDOREDUCTASE; TRYPTOPHAN HALOGENASE;

BIOLOGY;

AROMATIC SUBSTITUTION; ARTICLE; BIOSYNTHESIS; CHLORINATION; CRYSTAL STRUCTURE; DECOMPOSITION; ENZYME CHEMISTRY; ENZYME MECHANISM; ENZYME STRUCTURE; PRIORITY JOURNAL; STEREOCHEMISTRY; SUBSTITUTION REACTION; AMINO ACID SEQUENCE; BINDING SITE; CHEMICAL STRUCTURE; CHEMISTRY; DIMERIZATION; ENZYMOLOGY; HYDROGEN BOND; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR GENETICS; OXIDATION REDUCTION REACTION; PROTEIN CONFORMATION; PROTEIN SECONDARY STRUCTURE; PROTEIN TERTIARY STRUCTURE; PSEUDOMONAS FLUORESCENS; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BINDING SITES; CHLORIDES; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; FLAVIN-ADENINE DINUCLEOTIDE; HYDROGEN BONDING; HYPOCHLOROUS ACID; INDOLES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; OXIDATION-REDUCTION; OXIDOREDUCTASES; OXYGEN; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS FLUORESCENS; TRYPTOPHAN;

EID: 25844517049     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1116510     Document Type: Article

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34. J.H.N. is a Biotechnology and Biological Sciences Research Council (BBSRC) career development fellow. We thank J. Farack for chlorination of 5-methylindole with HOCl and M. Dorward for technical assistance. This work was supported by grants from the BBSRC (J.H.N.), the Wellcome Trust (J.H.N.), the Deutsche Forschungsgemeinschaft [Pe 348/17-1 and 17-2 (K.H.V.P.)], the Sächsische Staatsministerium für Umwelt und Landwirtschaft (K.H.V.P.) and the Fonds der Chemischen Industrie (K.H.V.P.). We thank G. Taylor, D. O'Hagan, and S. Chapman for a critical review of the manuscript. The native structure was determined by the Scottish Structural Proteomics Facility funded by Scottish Higher Education Funding Council and BBSRC. We acknowledge the use of BM14 (UK) and thank M. Walsh for data collection assistance. The use of European Synchrotron Radiation Facility beamlines is acknowledged, as is the BBSRC circular dichroism spectroscopy center in Glasgow. Coordinates and data have been deposited in the Protein Database with codes 2APG, 2AQJ, 2ARD, and 2AR8.