A continuous fluorescent assay for protein prenyltransferases measuring diphosphate release

Analytical Biochemistry

Volumn 345, Issue 2, 2005, Pages 302-311

  Pais, June E ^a   Bowers, Katherine E ^b,c   Stoddard, Andrea K ^b   Fierke, Carol A ^a,b  

^a UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF MICHIGAN   (United States)

^c REGENERON PHARMACEUTICALS INC   (United States)

Author keywords

Diphosphate release; Phosphate binding protein; Protein farnesyltransferase; Protein geranylgeranyltransferase

Indexed keywords

AMINO ACIDS; CONTROLLED DRUG DELIVERY; ESCHERICHIA COLI; FLUORESCENCE; PROTEINS; RATE CONSTANTS;

CHEMOTHERAPEUTIC DRUGS; DIPHOSPHATES; GERANYLGERANYLTRANSFERASE; HIGH THROUGHPUT SCREENING; INORGANIC PYROPHOSPHATASES; PHOSPHATE-BINDING PROTEINS; PRENYL DIPHOSPHATE; PROTEIN FARNESYLTRANSFERASE;

TARGETED DRUG DELIVERY;

COUMARIN; DIMETHYLALLYLTRANSFERASE; INORGANIC PYROPHOSPHATASE; PHOSPHATE; PHOSPHATE BINDING PROTEIN; PYROPHOSPHATE; RAS PROTEIN;

ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE; HIGH THROUGHPUT SCREENING; PRENYLATION; PRIORITY JOURNAL;

ESCHERICHIA COLI;

EID: 25444515175     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2005.07.040     Document Type: Article

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