Sequence specificity and role of proximal amino acids of the histone H3 tail on catalysis of murine G9a lysine 9 histone H3 methyltransferase

Biochemistry

Volumn 44, Issue 39, 2005, Pages 12998-13006

  Chin, Hang Gyeong ^a   Pradhan, Mihika ^a   Estève, Pierre Olivier ^a   Patnaik, Debasis ^a   Evans Jr , Thomas C ^a   Pradhan, Sriharsa ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; ENZYMES; SUBSTRATES;

FUSION PROTEINS; HISTONES; PROXIMAL AMINO ACIDS;

AMINO ACIDS;

ALANINE; AMINO ACID; ENZYME; GLUTATHIONE TRANSFERASE; GREEN FLUORESCENT PROTEIN; HISTONE H3; HYBRID PROTEIN; LYSINE; METHYLTRANSFERASE; PROTEIN G9A; RECOMBINANT ENZYME; SERINE; THREONINE; UNCLASSIFIED DRUG;

ACETYLATION; AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME LOCALIZATION; ENZYME METABOLISM; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOPRECIPITATION; METHYLATION; MOUSE; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; STEADY STATE;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; ANIMALS; CATALYSIS; HISTONE-LYSINE N-METHYLTRANSFERASE; HISTONES; KINETICS; METHYLATION; MICE; PEPTIDE FRAGMENTS; PHOSPHORYLATION; S-ADENOSYLMETHIONINE; SUBSTRATE SPECIFICITY;

MURINAE;

EID: 25444471273     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi0509907     Document Type: Article

References (35)

1. Luger, K., Mader, A. W., Richmond, R. K., Sargent, D. F., and Richmond, T. J. (1997) Crystal structure of the nucleosome core particle at 2.8 Å resolution, Nature 389, 251-260.
2. Van Holde, K. E (1988) Chromatin, Springer-Verlag, New York.
3. Ehrenhofer-Murray, A. E. (2004) Chromatin dynamics at DNA replication, transcription and repair, Eur. J. Biochem. 271, 2335-2349.
4. Rice, J. C., and Allis, C. D. (2001) Histone methylation versus histone acetylation: new insights into epigenetic regulation, Curr. Opin. Cell Biol. 13, 263-273.
5. Prigent, C., and Dimitrov, S. (2003) Phosphorylation of serine 10 in histone H3, what for?, J. Cell Sci. 116, 3677-3685.
6. Jenuwein, T., and Allis, C. D. (2001) Translating the histone code, Science 293, 1074-1080.
7. Strahl, B. D., and Allis, C. D. (2000) The language of covalent histone modifications, Nature 403, 41-45.
8. Lachner, M., O'Sullivan, R. J., and Jenuwein, T. (2003) An epigenetic road map for histone lysine methylation, J. Cell Sci. 116, 2117-2124.
9. Xu, W., Cho, H., and Evans, R. M. (2003) Acetylation and methylation in nuclear receptor gene activation, Methods Enzymol. 364, 205-223.
10. Santos-Rosa, H., Schneider, R., Bannister, A. J., Sherriff, J., Bernstein, B. E., Emre, N. C., Schreiber, S. L., Mellor, J., and Kouzarides, T. (2002) Active genes are tri-methylated at K4 of histone H3, Nature 419, 407-411.
11. Bernstein, B. E., Kamal, M., Lindblad-Toh, K., Bekiranov, S., Bailey, D. K., Huebert, D. J., McMahon, S., Karlsson, E. K., Kulbokas, E. J., III, Gingeras, T. R., Schreiber, S. L., and Lander, E. S. (2005) Genomic maps and comparative analysis of histone modifications in human and mouse, Cell 120, 169-181.
12. Rea, S., Eisenhaber, F., O'Carroll, D., Strahl, B. D., Sun, Z. W., Schmid, M., Opravil, S., Mechtler, K., Ponting, C. P., Allis, C. D., and Jenuwein, T. (2000) Regulation of chromatin structure by site-specific histone H3 methyltransferases, Nature 406, 593-599.
13. Ivanova, A. V., Bonaduce, M. J., Ivanov, S. V., and Klar, A. J. (1998) The chromo and SET domains of the Clr4 protein are essential for silencing in fission yeast, Nat. Genet. 19, 192-195.
14. Schotta, G., Eben, A., Krauss, V., Fischer, A., Hoffmann, J., Rea, S., Jenuwein, T., Dorn, R., and Reuter, G. (2002) Central role of Drosophila SU(VAR)3-9 in histone H3-K9 methylation and heterochromatic gene silencing, EMBO J. 21, 1121-1131.
15. Peters, A. H., Kubicek, S., Mechtler, K., O'Sullivan, R. J., Derijck, A. A., Perez-Burgos, L., Kohlmaier, A., Opravil, S., Tachibana, M., Shinkai, Y., Martens, J. H., and Jenuwein, T. (2003) Partitioning and plasticity of repressive histone methylation States in mammalian chromatin, Mol. Cell 12, 1577-1589.
16. Tachibana, M., Sugimoto, K., Fukushima, T., and Shinkai, Y. (2001) Set domain-containing protein, G9a, is a novel lysine-preferring mammalian histone methyltransferase with hyperactivity and specific selectivity to lysines 9 and 27 of histone H3, J. Biol. Chem. 276, 25309-25317.
17. Tachibana, M., Sugimoto, K., Nozaki, M., Ueda, J., Ohta, T., Ohki, M., Fukuda, M., Takeda, N., Niida, H., Kato, H., and Shinkai, Y. (2002) G9a histone methyltransferase plays a dominant role in euchromatic histone H3 lysine 9 methylation and is essential for early embryogenesis, Genes Dev. 16, 1779-1791.
18. Zhang, X., Tamaru, H., Khan, S. I., Horton, J. R., Keefe, L. J., Selker, E. U., and Cheng, X (2002) Structure of the Neurospora SET domain protein DIM-5, a histone H3 lysine methyltransferase, Cell 111, 117-127.
19. Min, J., Zhang, X., Cheng, X., Grewal, S. I., and Xu, R. M. (2002) Structure of the SET domain histone lysine methyltransferase Clr4, Nat. Struct. Biol. 9, 828-832.
20. Trievel, R. C., Beach, B. M., Dirk, L. M., Houtz, R. L., and Hurley, J. H. (2002) Structure and catalytic mechanism of a SET domain protein methyltransferase, Cell 111, 91-103.
21. Wilson, J. R., Jing, C., Walker, P. A., Martin, S. R., Howell, S. A., Blackburn, G. M., Gamblin, S. J., and Xiao, B. (2002) Crystal structure and functional analysis of the histone methyltransferase SET7/9, Cell 111, 105-115.
22. Zhang, X., Yang, Z., Khan, S. I., Horton, J. R., Tamaru, H., Selker, E. U., and Cheng, X. (2003) Structural basis for the product specificity of histone lysine methyltransferases, Mol. Cell 12, 177-185.
23. Patnaik, D., Chin, H. G., Esteve, P. O., Benner, J., Jacobsen, S. E., and Pradhan, S. (2004) Substrate specificity and kinetic mechanism of mammalian G9a histone H3 methyltransferase, J. Biol. Chem. 279, 53248-53258.
24. Collins, R. E., Tachibana, M., Tamaru, H., Smith, K. M., Jia, D., Zhang, X., Selker, E. U., Shinkai, Y., and Cheng, X. (2005) In vitro and in vivo analyses of a Phe/Tyr switch controlling product specificity of histone lysine methyltransferases, J. Biol. Chem. 280, 5563-5570.
25. Estève, P. O., Patnaik, D., Chin, H. G., Benner, J., Teitell, M. A., and Pradhan, S. (2005) Functional analysis of the N- and C-terminus of mammalian G9a histone H3 methyltransferase, Nucleic Acids Res. 33, 3211-3223.
26. Estève, P. O., Chin, H. G., and Pradhan, S. (2005) Human maintenance DNA (cytosine-5)-methyltransferase and p53 modulate expression of p53-repressed promoters. Proc. Natl. Acad. Sci. U.S.A. 102, 1000-1005.
27. Silva, J., Mak, W., Zvetkova, I., Appanah, R., Nesterova, T. B., Webster, Z., Peters, A. H., Jenuwein, T., Otte, A. P., and Brockdorff, N. (2003) Establishment of histone h3 methylation on the inactive X chromosome requires transient recruitment of Eed-Enx1 polycomb group complexes, Dev. Cell 4, 481-495.
28. Plath, K., Talbot, D., Hamer, K. M., Otte, A. P., Yang, T. P., Jaenisch, R., and Panning, B. (2004) Developmentally regulated alterations in Polycomb repressive complex 1 proteins on the inactive X chromosome, J. Cell Biol. 167, 1025-1035.
29. Rice, J. C., Briggs, S. D., Ueberheide, B., Barber, C. M., Shabanowitz, J., Hunt, D. F., Shinkai, Y., and Allis, C. D. (2003) Histone methyltransferases direct different degrees of methylation to define distinct chromatin domains, Mol. Cell 12, 1591-1598.
30. Yeates, T. O. (2002) Structures of SET domain proteins: protein lysine methyltransferases make their mark, Cell 111, 5-7.
31. Peters, A. H., O'Carroll, D., Scherthan, H., Mechtler, K., Sauer, S., Schofer, C., Weipoltshammer, K., Pagani, M., Lachner, M., Kohlmaier, A., Opravil, S., Doyle, M., Sibilia, M., Jenuwein, T. (2001) Loss of the Suv39h histone methyltransferases impairs mammalian heterochromatin and genome stability, Cell 107, 323-337.
32. Roopra, A., Qazi, R., Schoenike, B., Daley, T. J., and Morrison, J. F. (2004) Localized domains of G9a-mediated histone methylation are required for silencing of neuronal genes, Mol. Cell 14, 727-738.
33. Gyory, I., Wu, J., Fejer, G., Seto, E., and Wright, K. L. (2004) PRDI-BF1 recruits the histone H3 methyltransferase G9a in transcriptional silencing, Nat. Immunol. 5, 299-308.
34. Shi, Y., Lan, F., Matson, C., Mulligan, P., Whetstine, J. R., Cole, P. A., Casero, R. A., and Shi, Y. (2000) Histone demethylation mediated by the nuclear amine oxidase homolog LSD1, Cell 119, 941-953.
35. Metzger et al. (2005) Nature (Aug 3, Epub).