Mechanistic insights into the isochorismate pyruvate lyase activity of the catalytically promiscuous PchB from combinatorial mutagenesis and selection

Journal of Biological Chemistry

Volumn 280, Issue 38, 2005, Pages 32827-32834

  Künzler, Dominik E ^a   Sasso, Severin ^a   Gamper, Marianne ^a   Hilvert, Donald ^a   Kast, Peter ^a  

^a ETH ZURICH   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CATALYSIS; MOLECULAR BIOLOGY; MOLECULAR STRUCTURE; MUTAGENESIS;

ELECTROSTATIC CATALYSIS; HOMOLOGY; RANDOMIZATION; REACTION MECHANISM;

ENZYMES;

ARGININE; CHORISMATE MUTASE; CHORISMIC ACID; GLUTAMIC ACID; GLUTAMINE; ISOCHORISMATE PYRUVATE LYASE; LYASE; PROTEIN PCHB; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CELL VIABILITY; ELECTRICITY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME MECHANISM; EVOLUTION; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTON TRANSPORT; PSEUDOMONAS AERUGINOSA;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BINDING SITES; CATALYSIS; CELL PROLIFERATION; CHORISMATE MUTASE; CIRCULAR DICHROISM; DNA-DIRECTED RNA POLYMERASES; DOSE-RESPONSE RELATIONSHIP, DRUG; ESCHERICHIA COLI; EVOLUTION, MOLECULAR; GENE EXPRESSION REGULATION; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE LIBRARY; GENETIC TECHNIQUES; GLUTAMIC ACID; GLUTAMINE; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, BIOLOGICAL; MODELS, CHEMICAL; MOLECULAR SEQUENCE DATA; MUTAGENESIS; MUTAGENESIS, SITE-DIRECTED; MUTATION; OXO-ACID-LYASES; PLASMIDS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; PSEUDOMONAS AERUGINOSA; SEQUENCE HOMOLOGY, AMINO ACID; SPECTROPHOTOMETRY; VIRAL PROTEINS;

PSEUDOMONAS AERUGINOSA;

EID: 25444445359     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M506883200     Document Type: Article

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