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Volumn 121, Issue 8, 1999, Pages 1756-1757

Heavy atom isotope effects reveal a highly polarized transition state for chorismate mutase [5]

Author keywords

[No Author keywords available]

Indexed keywords

CHORISMIC ACID; PHOSPHOENOLPYRUVATE; PHOSPHOTRANSFERASE; SYNTHETASE;

EID: 0033518886     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja9841759     Document Type: Letter
Times cited : (83)

References (33)
  • 18
    • 0344519795 scopus 로고    scopus 로고
    • note
    • 0 was determined by running the rearrangement reaction to completion (3 h) with wild-type BsCM at 2 mM chorismate and determining the isotope ratio on the mass spectrometer (experiment done in triplicate). The fractional conversion ∫ was determined by measuring the UV absorbance of the chorismate chromophore at 275 nm of the reaction mixture at the endpoint.
  • 21
    • 0345382057 scopus 로고    scopus 로고
    • note
    • 14C isotope effects.
  • 23
    • 0031360609 scopus 로고    scopus 로고
    • An alternative two-step mechanism involving an ion pair intermediate cannot be ruled out with complete confidence. Such an ion pair could only be an intermediate if the partition ratio forward is small, however, which seems unlikely in light of the strongly exothermic nature of the reaction (Kast, P.; Tewari, Y. B.; Wiest, O.; Hilvert, D.; Houk, K. N.; Goldberg, R. N. J. Phys. Chem. B 1997, 101, 10976-10982).
    • (1997) J. Phys. Chem. B , vol.101 , pp. 10976-10982
    • Kast, P.1    Tewari, Y.B.2    Wiest, O.3    Hilvert, D.4    Houk, K.N.5    Goldberg, R.N.6
  • 26
    • 33845375092 scopus 로고
    • and references therein
    • Normal hydrogen bonding probably has only a minor effect on the fractionation factor of an oxygen (the bond order is low), while full protonation has about a 2% effect (Knight, W. B., Weiss, P. M.; Cleland, W. W. J. Am. Chem. Soc. 1986, 108, 2759-2761 and references therein).
    • (1986) J. Am. Chem. Soc. , vol.108 , pp. 2759-2761
    • Knight, W.B.1    Weiss, P.M.2    Cleland, W.W.3
  • 31
    • 0344088558 scopus 로고    scopus 로고
    • personal communication
    • Singleton, D. A., personal communication.
    • Singleton, D.A.1
  • 32
    • 0344519794 scopus 로고    scopus 로고
    • note
    • 7,20 the experimental isotope effects must be considered minimum values.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.