Is chorismate mutase a prototypic entropy trap? - Activation parameters for the Bacillus subtilis enzyme

Tetrahedron Letters

Volumn 37, Issue 16, 1996, Pages 2691-2694

  Kast, Peter ^a   Asif Ullah, M ^a   Hilvert, Donald ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CHORISMATE MUTASE;

ARTICLE; BACILLUS SUBTILIS; ENZYME ACTIVATION; THERMODYNAMICS;

EID: 0029928213     PISSN: 00404039     EISSN: None     Source Type: Journal    
DOI: 10.1016/0040-4039(96)00338-3     Document Type: Article

References (32)

1. Haslam, E. Shikimic Acid: Metabolism and Metabolites; John Wiley & Sons: New York, 1993.
2. Andrews, P. R.; Cain, E. N.; Rizzardo, E.; Smith, G. D. Biochemistry 1977, 16, 4848-4852.
3. Sogo, S. G.; Widlanski, T. S.; Hoare, J. H.; Grimshaw, C. E.; Berchtold, G. A.; Knowles, J. R. J. Am. Chem. Soc. 1984, 106, 2701-2703.
4. Copley, S. D.; Knowles, J. R. J. Am. Chem. Soc. 1985, 107, 5306-5308.
5. Lee, A. Y.; Stewart, J. D.; Clardy, J.; Ganem, B. Chem. Biol. 1995, 2, 195-203.
6. Görisch, H. Biochemistry 1978, 17, 3700-3705.
7. Gray, J. V.; Golinelli-Pimpaneau, B.; Knowles, J. R. Biochemistry 1990, 29, 376-383.
8. Chook, Y. M.; Ke, H.; Lipscomb, W. N. Proc. Natl. Acad. Sci. USA 1993, 90, 8600-8603.
9. Chook, Y. M.; Gray, J. V.; Ke, H.; Lipscomb, W. N. J. Mol. Biol. 1994, 240, 476-500.
10. We noticed that chorismate mutase is easily released from E. coli cells when treated with high osmolarity buffer [20% (w/v) sucrose/30 mM Tris-HCl, pH 8.0/1 mM EDTA]. The highly enriched chorismate mutase fraction was chromatographed on DEAE DE-52 cellulose and then Sephadex G-75. A detailed protocol will be published elsewhere. Electrospray mass spectrometric analysis gave a subunit mass of 14487 ± 2 Da (calculated mass = 14489 Da). A prominent signal at 14357 Da (≈ 25% of 14487 peak) corresponds to a processed protein with cleaved N-terminal methionine. We believe that this truncation (seen in independent purifications and in samples from outside sources) occurs in vivo during protein synthesis and does not affect our results.
11. Rajagopalan, J. S.; Taylor, K. M.; Jaffe, E. K. Biochemistry 1993, 32, 3965-3972.
12. KA12 was constructed by generalized P1 transduction to delete the recA gene from strain KB224 (Balakrishnan, R.; Backman, K. Gene 1988, 67, 97-103.) according to a procedure described earlier: Kast, P.; Hennecke, H. J. Mol. Biol. 1991, 222, 99-124.
13. KA12 was constructed by generalized P1 transduction to delete the recA gene from strain KB224 (Balakrishnan, R.; Backman, K. Gene 1988, 67, 97-103.) according to a procedure described earlier: Kast, P.; Hennecke, H. J. Mol. Biol. 1991, 222, 99-124.
14. 1H-NMR, analytical HPLC and, before each experiment, by quantitative conversion by chorismate mutase.
15. Chorismate mutase concentration was determined using the Pierce BCA Protein Assay Reagent (Pierce, Rockford, IL) with bovine serum albumin as the calibration standard.
16. Connors, K. A. Chemical Kinetics. The Study of Reaction Rates in Solution; VCH Publishers: New York, 1990; pp. 187-243.
17. Andrews, P. R.; Smith, G. D.; Young, I. G. Biochemistry 1973, 12, 3492-3498.
18. Jackson, D. Y.; Liang, M. N.; Bartlett, P. A.; Schultz, P. G. Angew. Chem. Int. Ed. Engl. 1992, 31, 182-183.
19. Hilvert, D.; Carpenter, S. H.; Nared, K. D.; Auditor, M.-T. M. Proc. Natl. Acad. Sci. USA 1988, 85, 4953-4955.
20. Gray, J. V.; Eren, D.; Knowles, J. R. Biochemistry 1990, 29, 8872-8878.
21. Campbell, A. P.; Tarasow, T. M.; Massefski, W.; Wright, P. E.; Hilvert, D. Proc. Natl. Acad. Sci. USA 1993, 90, 8663-8667.
22. Haynes, M. R.; Stura, E. A.; Hilvert, D.; Wilson, I. A. Science 1994, 263, 646-652.
23. Westheimer, F. H. Adv. Enzymol. Relat. Areas Mol. Biol. 1962, 24, 441-482.
24. Addadi, L.; Jaffe, E. K.; Knowles, J. R. Biochemistry 1983, 22, 4494-4501.
25. Copley, S. D.; Knowles, J. R. J. Am. Chem. Soc. 1987, 109, 5008-5013.
26. Gajewski, J. J.; Jurayj, J.; Kimbrough, D. R.; Gande, M. E.; Ganem, B.; Carpenter, B. K. J. Am. Chem. Soc. 1987, 109, 1170-1186.
27. Severance, D. L.; Jorgensen, W. L. J. Am. Chem. Soc. 1992, 114, 10966-10968.
28. Wiest, O.; Houk, K. N. J. Org. Chem. 1994, 59, 7582-7584.
29. Lee, A. Y.; Karplus, P. A.; Ganem, B.; Clardy, J. J. Am. Chem. Soc. 1995, 117, 3627-3628.
30. Xue, Y.; Lipscomb, W. N. Proc. Natl. Acad. Sci. USA 1995, 92, 10595-10598.
31. Kast, P.; Hartgerink, J. D.; Asif-Ullah, M.; Hilvert, D. J. Am. Chem. Soc. 1996, 118, in press.
32. Kast, P.; Asif-Ullah, M.; Jiang, N.; Hilvert, D. Proc. Natl. Acad. Sci. USA 1996, in press.