Stable hybridoma cultivation in a pilot-scale acoustic perfusion system: Long-term process performance and effect of recirculation rate

Biotechnology and Bioengineering

Volumn 91, Issue 7, 2005, Pages 894-900

  Dalm, Marcella C F ^a,b   Jansen, Menno ^b   Keijzer, Timo M P ^c   Van Grunsven, Wout M J ^b   Oudshoorn, Arthur ^c   Tramper, Johannes ^a   Martens, Dirk E ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

^b Small Scale Cell Culture and Development   (Netherlands)

^c Deer Park Business Centre   (Netherlands)

Author keywords

Acoustic perfusion; Hybridoma; Monoclonal antibody; Nutrient gradients; Pilot scale; Productivity

Indexed keywords

ANTIBODIES; CORRELATION METHODS; DRUG PRODUCTS; METABOLISM; NUTRITION; OXYGEN; PARAMETER ESTIMATION; PRODUCTIVITY;

ACOUSTIC PERFUSION; HYBRIDOMA; MONOCLONAL ANTIBODY; NUTRIENT GRADIENTS; PILOT-SCALE;

CELLS;

AMMONIA; GLUCOSE; GLUTAMINE; IMMUNOGLOBULIN G1; LACTATE DEHYDROGENASE; LACTIC ACID; MONOCLONAL ANTIBODY;

ANIMAL CELL; ARTICLE; BIOREACTOR; CELL GROWTH; ENZYME LINKED IMMUNOSORBENT ASSAY; GROWTH RATE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYBRIDOMA CELL CULTURE; MOUSE; NONHUMAN; NUTRIENT;

ANTIBODIES, MONOCLONAL; BIOREACTORS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL SURVIVAL; EFFICIENCY; FERMENTATION; GLUCOSE; GLUTAMINE; HYBRIDOMAS; LACTIC ACID; OXYGEN; PERFUSION;

EID: 25144500122     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20552     Document Type: Article

References (21)

1. Arai T, Yokohama S, Tokashiki M. 1993. 50 L scale perfusion culture of hybridoma cells by gravitational settling for cell separation. In: Kaminogawa S, Ametani A, Hachimura S, editors. Animal cell technology: Basic applied aspects. Dordrecht: Kluwer academic publishers, pp 341-346.
2. Castilho LR, Medronho RA. 2002. Cell retention devices for suspended-cell perfusion cultures. In: Scheper Th, editor. Advances in biochemical engineering/biotechnology, vol. 74. Heidelberg: Springer-Verlag, pp 129-169.
3. Dalm MCF, Cuijten SMR, van Grunsven WMJ, Tramper J, Martens DE. 2004. Effect of feed and bleed rate on hybridoma cells in an acoustic perfusion bioreactor: Part I Cell density, viability and cell-cycle distribution. Biotechnol Bioeng 88:547-557.
4. Deo YM, Mahadevan MD, Fuchs R. 1996. Practical considerations in operations and scale-up of spinfilter based bioreactors for monoclonal antibody production. Biotechnol Prog 12:157-164.
5. Doblhoff-Dier O, Gaida Th, Katinger H. 1994. A novel ultrasonic resonance field device for the retention of animal cells. Biotechnol Prog 10:428-432.
6. Follstad BD, Balcarel ER, Stephanopoulos G, Wang DIC. 1999. Metabolic flux analysis of hybridoma continuous culture steady state multiplicity. Biotechnol Bioeng 63:675-683.
7. Gaida Th, Doblhoff-Dier O, Strutzenberger K, Katinger H, Burger W, Groeschl M, Handle B, Benes E. 1996. Selective retention of viable cells in ultrasonic resonance field devices. Biotechnol Prog 12:73-76.
8. Gorenflo VM, Smith L, Dedinsky B, Persson B, Piret JM. 2002. Scale-up and optimization of an acoustic filter for 200 L/day perfusion of a CHO cell culture. Biotechnol Bioeng 80:438-444.
9. Herbert P, Barros P, Ratola N, Alves A. 2000. HPLC determination of amino acids in musts and port wine using OPA/FMOC derivatives. J Food Science 65:1130-1133.
10. Hiller GW, Aeschlimann AD, Clark DC, Blanch HW. 1991. A kinetic analysis of hybridoma growth and metabolism in continuous suspension culture on serum-free medium. Biotechnol Bioeng 38:733-741.
11. Johnson M, Lanthier S, Massie B, Lefebvre G, Kamen AA. 1996. Use of the centritech lab centrifuge for perfusion culture of hybridoma cells in protein-free medium. Biotechnol Prog 12:855-864.
12. Marks DM. 2003. Equipment design considerations for large scale cell culture. Cytotechnol 42:21-33.
13. Miller WM, Blanch HW, Wilke CR. 1988. A kinetic analysis of hybridoma growth and metabolism in batch and continuous culture: Effect of nutrient concentration, dilution rate and pH. Biotechnol Bioeng 32:947-965.
14. Rhiel M, Murhammer DW. 1995. The effect of oscillating dissolved oxygen concentrations on the metabolism of a Spodoptera frugiperda IPLB-Sf21-AE clonal isolate. Biotechnol Bioeng 47:640-650.
15. Ryll T, Dutina G, Reyes A, Gunson J, Krummen L, Etcheverry T. 2000. Performance of small-scale CHO perfusion cultures using an acoustic cell filtration device for cell retention: Characterization of separation efficiency and impact of perfusion on product quality. Biotechnol Bioeng 69:440-449.
16. Takamatsu H, Hamamoto K, ishimaru K, Yokoyama S, Tokashiki M. 1996. Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones. Appl Microbiol Biotechnol 45:454-457.
17. Trampler F, Sonderhoff SA, Pui PWS, Kilburn DG, Piret JM. 1994. Acoustic cell filter for high density perfusion culture of hybridoma cells. Bio/Technol 12:281-284.
18. Voisard D, Meuwly F, Ruffieux PA, Baer G, Kadouri A. 2003. Potential of cell retention techniques for large-scale high-density perfusion culture of suspended mammalian cells. Biotechnol Bioeng 82:751-765.
19. Werner RG. 2004. Economics aspects of commercial manufacture of biopharmaceuticals. J Biotechnol 113:171-182.
20. Woodside S, Bowen BD, Piret JM. 1998. Mammalian cell retention devices for stirred perfusion bioreactors. Cytotechnol 28:163-175.
21. Yabannavar VM, Singh V, Connelly NV. 1994. Scale-up of spinfilter perfusion bioreactor for mammalian cell retention. Biotechnol Bioeng 43:159-164.