Large-scale perfusion culture process for suspended mammalian cells that uses a centrifuge with multiple settling zones

Applied Microbiology and Biotechnology

Volumn 45, Issue 4, 1996, Pages 454-457

  Takamatsu, H ^a   Hamamoto, K ^a   Ishimaru, K ^a   Yokoyama, S ^a   Tokashiki, M ^a  

^a TEIJIN LTD   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

MAMMALIA; ANIMALIA;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGY; CELL CULTURE; CENTRIFUGE; CONTROLLED STUDY; MAMMAL; MOUSE; NONHUMAN; PERFUSION;

ANIMALS; CELL COUNT; CELL CULTURE TECHNIQUES; CELL SEPARATION; CELLS, CULTURED; CENTRIFUGATION; EQUIPMENT DESIGN; FEASIBILITY STUDIES; FLUOROCARBON POLYMERS; HUMANS; HYBRIDOMAS; MAMMALS; MICE; PERFUSION;

EID: 0030015158     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF00578455     Document Type: Article

References (8)

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2. Feder J, Tolbert WR (1983) The large-scale cultivation of mammalian cells. Scientific American 248:24-31
3. Hamamoto K, Ishimaru K, Tokashiki M (1989) Perfusion culture of hybridoma cells using a centrifuge to separate cells from culture mixture. J Ferment Bioeng 67:190-194
4. Himmelfarb P, Thayer PS, Martin HE (1969) Spin filler culture: the propagation of mammalian cells in suspension. Science 164:555-557
5. Jäger V (1992) High density perfusion culture of animal cells using a novel continuous flow centrifuge. In: Murakami H. Shirahata S, Tachibana H (eds) Animal cell technology: basic and applied aspects, Kluwer, Dordrecht, pp 209-216
6. Murakami H, Shimomura T, Nakamura T, Ohashi H, Shinohara K, Omura H (1984) Development of a basal medium for serum-free cultivation of hybridoma cells in high density. J Agric Chem Soc Jpn 58:575-583
7. Tokashiki M, Arai T (1989) High-density culture of mouse-human hybridoma cells using a perfusion culture apparatus with multisettling zones to separate cells from the culture medium. Cytotechnology 2:5-8
8. Tokashiki M, Arai T, Hamamoto K, Ishimaru K (1990) High density culture of hybridoma cells using a perfusion culture vessel with an external centrifuge. Cytotechnology 3:239-244