Performance of small-scale CHO perfusion cultures using an acoustic cell filtration device for cell retention: Characterization of separation efficiency and impact of perfusion on product quality

Biotechnology and Bioengineering

Volumn 69, Issue 4, 2000, Pages 440-449

  Ryll, Thomas ^a   Dutina, George ^a   Reyes, Arthur ^a   Gunson, Jane ^a   Krummen, Lynne ^a   Etcheverry, Tina ^a  

^a GENENTECH INC   (United States)

Author keywords

Acoustic filtration; Chinese hamster ovary (CHO) cell culture; Perfusion; Product quality; Proteolysis; Separation efficiency

Indexed keywords

LEUPEPTIN; TISSUE PLASMINOGEN ACTIVATOR; TNK TISSUE PLASMINOGEN ACTIVATOR; TNK-TISSUE PLASMINOGEN ACTIVATOR;

ACOUSTICS; ANIMAL; ARTICLE; BIOSYNTHESIS; CELL SURVIVAL; CHO CELL; CULTURE TECHNIQUE; CYTOLOGY; FILTRATION; HAMSTER; INSTRUMENTATION; MACACA; METABOLISM; METHODOLOGY; PERFUSION; WESTERN BLOTTING;

ACOUSTICS; ANIMALS; BLOTTING, WESTERN; CELL CULTURE TECHNIQUES; CELL SURVIVAL; CHO CELLS; CRICETINAE; FILTRATION; LEUPEPTINS; MACACA MULATTA; PERFUSION; TISSUE PLASMINOGEN ACTIVATOR;

BATCH CELL CULTURE; BIOREACTORS; CENTRIFUGATION; ENZYMES; FILTRATION; GROWTH KINETICS;

ACOUSTIC CELL FILTRATION DEVICE; CELL RETENTION; CHINESE HAMSTER OVARY; PERFUSION CULTURES; PRODUCT QUALITY; PROTEOLYSIS; SEPARATION EFFICIENCY;

ANIMAL CELL CULTURE;

EID: 0034238099     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20000820)69:4<440::AID-BIT10>3.0.CO;2-0     Document Type: Article

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