Visualization of inositol phosphate-dependent mobility of Ku: Depletion of the DNA-PK cofactor InsP6 inhibits Ku mobility

Nucleic Acids Research

Volumn 32, Issue 9, 2004, Pages 2776-2784

  Byrum, Jennifer ^b   Jordan, Stephen ^b   Safrany, Stephen T ^a   Rodgers, William ^b  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

^b OKLAHOMA MEDICAL RESEARCH FOUNDATION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CALMODULIN INHIBITOR; CHLORPROMAZINE; GREEN FLUORESCENT PROTEIN; INOSITOL PHOSPHATE; KU ANTIGEN; N (6 AMINOHEXYL) 5 CHLORO 1 NAPHTHALENESULFONAMIDE; PHYTIC ACID; CALMODULIN; CELL NUCLEUS ANTIGEN; DNA BINDING PROTEIN; DNA DEPENDENT PROTEIN KINASE; NUCLEAR PROTEIN; PRKDC PROTEIN, HUMAN; PROTEIN SERINE THREONINE KINASE; SULFONAMIDE;

AMINO TERMINAL SEQUENCE; ARTICLE; BLEACHING; CONTROLLED STUDY; CULTURE MEDIUM; DELETION MUTANT; DEPLETION; DIFFUSION; DIMERIZATION; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN LOCALIZATION; BINDING SITE; CELL LINE; CHEMICAL STRUCTURE; CHEMISTRY; COENZYME; DRUG ANTAGONISM; DRUG EFFECT; GENE DELETION; GENETICS; HELA CELL; METABOLISM; PROTEIN BINDING; PROTEIN TERTIARY STRUCTURE; PROTEIN TRANSPORT;

MAMMALIA;

ANTIGENS, NUCLEAR; BINDING SITES; CALMODULIN; CELL LINE; CHLORPROMAZINE; COENZYMES; DIMERIZATION; DNA-ACTIVATED PROTEIN KINASE; DNA-BINDING PROTEINS; HELA CELLS; HUMANS; MODELS, MOLECULAR; NUCLEAR PROTEINS; PHYTIC ACID; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; PROTEIN TRANSPORT; PROTEIN-SERINE-THREONINE KINASES; SEQUENCE DELETION; SULFONAMIDES;

EID: 2442637866     PISSN: 03051048     EISSN: None     Source Type: Journal    
DOI: 10.1093/nar/gkh592     Document Type: Article

References (45)

1. Smith,G.C. and Jackson,S.P. (1999) The DNA-dependent protein kinase. Genes Dev., 13, 916-934.
2. Mimori,T. and Hardin,J.A. (1986) Mechanism of interaction between Ku protein and DNA. J. Biol. Chem., 261, 10375-10379.
3. Paillard,S. and Strauss,F. (1991) Analysis of the mechanism of interaction of simian Ku protein with DNA. Nucleic Acids Res., 19, 5619-5624.
4. Ono,M., Tucker,P.W. and Capra,J.D. (1994) Production and characterization of recombinant human Ku antigen. Nucleic Acids Res., 22, 3918-3924.
5. Ono,M., Tucker,P.W. and Capra,J.D. (1996) Ku is a general inhibitor of DNA-protein complex formation and transcription. Mol. Immunol., 33, 787-796.
6. Dynan,W.S. and Yoo,S. (1998) Interaction of Ku protein and DNA-dependent protein kinase catalytic subunit with nucleic acids. Nucleic Acids Res., 26, 1551-1559.
7. Dvir,A., Peterson,S.R., Knuth,M.W., Lu,H. and Dynan,W.S. (1992) Ku autoantigen is the regulatory component of a template-associated protein kinase that phosphorylates RNA polymerase II. Proc. Natl Acad. Sci. USA, 89, 11920-11924.
8. Gottlieb,T.M. and Jackson,S.P. (1993) The DNA-dependent protein kinase: requirement for DNA ends and association with Ku antigen. Cell, 72, 131-142.
9. Grawunder,U., Wilm,M., Wu,X., Kulesza,P., Wilson,T.E., Mann,M. and Lieber,M.R. (1997) Activity of DNA ligase IV stimulated by complex formation with XRCC4 protein in mammalian cells. Nature, 388, 492-495.
10. Ramsden,D.A. and Gellert,M. (1998) Ku protein stimulates DNA end joining by mammalian DNA ligases: a direct role for Ku in repair of DNA double-strand breaks. EMBO J., 17, 609-614.
11. Gu,Y., Jin,S., Gao,Y., Weaver,D.T. and Alt,F.W. (1997) Ku70-deficient embryonic stem cells have increased ionizing radiosensitivity, defective DNA end-binding activity and inability to support V(D)J recombination. Proc. Natl Acad. Sci. USA, 94, 8076-8081.
12. Manis,J.P., Gu,Y., Lansford,R., Sonoda,E., Ferrini,R., Davidson,L., Rajewsky,K. and Alt,F.W. (1998) Ku70 is required for late B cell development and immunoglobulin heavy chain class switching. J. Exp. Med., 187, 2081-2089.
13. Nussenzweig,A., Chen,C., da Costa Soares,V., Sanchez,M., Sokol,K., Nussenzweig,M.C. and Li,G.C. (1996) Requirement for Ku80 in growth and immunoglobulin V(D)J recombination. Nature, 382, 551-555.
14. Getts,R.C. and Stamato,T.D. (1994) Absence of a Ku-like DNA end binding activity in the xrs double-strand DNA repair-deficient mutant. J. Biol. Chem., 269, 15981-15984.
15. Smider,V., Rathmell,W.K., Lieber,M.R. and Chu,G. (1994) Restoration of X-ray resistance and V(D)J recombination in mutant cells by Ku cDNA. Science, 266, 288-291.
16. Taccioli,G.E., Gottlieb,T.M., Blunt,T., Priestley,A., Demengeot,J., Mizuta,R., Lehmann,A.R., Alt,F.W., Jackson,S.P. and Jeggo,P.A. (1994) Ku80: product of the XRCC5 gene and its role in DNA repair and V(D)J recombination. Science, 265, 1442-1445.
17. Walker,J.R., Corpina,R.A. and Goldberg,J. (2001) Structure of the Ku heterodimer bound to DNA and its implications for double-strand break repair. Nature, 412, 607-614.
18. Rodgers,W., Jordan,S.J. and Capra,J.D. (2002) Transient association of Ku with nuclear substrates characterized using fluorescence photobleaching. J. Immunol., 168, 2348-2355.
19. Phair,R.D. and Misteli,T. (2000) High mobility of proteins in the mammalian cell nucleus. Nature, 404, 604-609.
20. McNally,J.G., Muller,W.G., Walker,D., Wolford,R. and Hager,G.L. (2000) The glucocorticoid receptor: rapid exchange with regulatory sites in living cells. Science, 287, 1262-1265.
21. Chen,D. and Huang,C. (2001) Nucleolar components involved in ribosome biogenesis cycle between the nucleolus and nucleoplasm in interphase cells. J. Cell Biol., 153, 169-176.
22. Boisvert,F.M., Kruhlak,M.J., Box,A.K., Hendzel,M.J. and Bazett-Jones,D.P. (2001) The transcription coactivator cbp is a dynamic component of the promyelocytic leukemia nuclear body. J. Cell Biol., 152, 1099-1106.
23. Hanakahi,L.A., Bartlet-Jones,M., Chappell,C., Pappin,D. and West,S.C. (2000) Binding of inositol phosphate to DNA-PK and stimulation of double-strand break repair. Cell, 102, 721-729.
24. Hanakahi,L.A. and West,S.C. (2002) Specific interaction of IP6 with human Ku70/80, the DNA-binding subunit of DNA-PK. EMBO J., 21, 2038-2044.
25. Ma,Y. and Lieber,M.R. (2002) Binding of inositol hexakisphosphate, (IP6) to Ku but not to DNA-PKcs. J. Biol. Chem., 277, 10756-10759.
26. Berridge,M.J. and Irvine,R.F. (1989) Inositol phosphates and cell signalling. Nature, 341, 197-205.
27. Dewaste,V., Pouillon,V., Moreau,C., Shears,S., Takazawa,K. and Erneux,C. (2000) Cloning and expression of a cDNA encoding human inositol 1,4,5-trisphosphate 3-kinase C. Biochem. J., 352, 343-351.
28. Minor,D.L.,Jr and Kim,P.S. (1994) Measurement of the beta-sheet-forming propensities of amino acids. Nature, 367, 660-663.
29. Laemmli,U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680-685.
30. Safrany,S.T. and Shears,S.B. (1998) Turnover of bis-diphosphoinositol tetrakisphosphate in a smooth muscle cell line is regulated by beta2-adrenergic receptors through a cAMP-mediated, A-kinase-independent mechanism. EMBO J., 17, 1710-1716.
31. Axelrod,D., Koppel,D.E., Schlessinger,J., Elson,E. and Webb,W.W. (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys. J., 16, 1055-1069.
32. Koike,M., Ikuta,T., Miyasaka,T. and Shiomi,T. (1999) The nuclear localization signal of the human Ku70 is a variant bipartite type recognized by the two components of nuclear pore-targeting complex. Exp. Cell Res., 250, 401-413.
33. Shamsuddin,A.M. and Yang,G.Y. (1995) Inositol hexaphosphate inhibits growth and induces differentiation of PC-3 human prostate cancer cells. Carcinogenesis, 16, 1975-1979.
34. Jin,S. and Weaver,D.T. (1997) Double-strand break repair by Ku70 requires heterodimerization with Ku80 and DNA binding functions. EMBO J., 16, 6874-6885.
35. Wu,X. and Lieber,M.R. (1996) Protein-protein and protein-DNA interaction regions within the DNA end-binding protein Ku70-Ku86. Mol. Cell Biol., 16, 5186-5193.
36. Sawada,M., Hayes,P., Matsuyama,S., Sun,W., Leskov,K. and Boothman,D.A. (2003) Cytoprotective membrane-permeable peptides designed from the Bax-binding domain of Ku70 suppresses the apoptotic translocation of Bax to mitochondria. Nature Cell Biol., 5, 352-357.
37. Sawada,M., Sun,W., Hayes,P., Leskov,K., Boothman,D.A. and Matsuyama,S. (2003) Ku70 suppresses the apoptotic translocation of Bax to mitochondria. Nature Cell Biol., 5, 320-329.
38. Lippincott-Schwartz,J., Snapp,E. and Kenworthy,A. (2001) Studying protein dynamics in living cells. Nature Rev. Mol. Cell Biol., 2, 444-456.
39. Szwergold,B.S., Graham,R.A. and Brown,T.R. (1987) Observation of inositol pentakis- and hexakis-phosphates in mammalian tissues by 31P NMR. Biochem. Biophys. Res. Commun., 149, 874-881.
40. Orchiston,E.A., Bennett,D., Leslie,N.R., Clarke,R.G., Winward,L., Downes,C.P. and Safrany,S.T. (2004) PTEN M-CBR3, a versatile and selective regulator of Ins(1,3,4,5,6)P5. Evidence for Ins(1,3,4,5,6)P5 as a proliferative signal. J. Biol. Chem., 279, 1116-1122.
41. York,J.D., Odom,A.R., Murphy,R., Ives,E.B. and Wente,S.R. (1999) A phospholipase C-dependent inositol polyphosphate kinase pathway required for efficient messenger RNA export. Science, 285, 96-100.
42. Odom,A.R., Stahlberg,A., Wente,S.R. and York,J.D. (2000) A role for nuclear inositol 1,4,5-trisphosphate kinase in transcriptional control. Science, 287, 2026-2029.
43. Saiardi,A., Caffrey,J.J., Snyder,S.H. and Shears,S.B. (2000) Inositol polyphosphate multikinase (ArgRIII) determines nuclear mRNA export in Saccharomyces cerevisiae. FEBS Lett., 468, 28-32.
44. Messenguy,F. and Dubois,E. (1993) Genetic evidence for a role for MCM1 in the regulation of arginine metabolism in Saccharomyces cerevisiae. Mol. Cell Biol., 13, 2586-2592.
45. Luo,H.R., Saiardi,A., Yu,H., Nagata,E., Ye,K. and Snyder,S.H. (2002) Inositol pyrophosphates are required for DNA hyperrecombination in protein kinase c1 mutant yeast. Biochemistry, 41, 2509-2515.