A high-throughput method for development of FRET-based indicators for proteolysis

Biochemical and Biophysical Research Communications

Volumn 319, Issue 1, 2004, Pages 72-77

  Nagai, Takeharu ^a,b   Miyawaki, Atsushi ^a  

^a RIKEN BRAIN SCIENCE INSTITUTE   (Japan)

^b Japan Science and Technology Agency   (Japan)

Author keywords

Apoptosis; Fluorescence resonance energy transfer; Green fluorescent protein; High throughput screening; Imaging; Protease

Indexed keywords

CASPASE 3; CYAN FLUORESCENT PROTEIN; YELLOW FLUORESCENT PROTEIN;

APOPTOSIS; ARTICLE; BACTERIUM COLONY; CARCINOMA CELL; ENZYME ACTIVATION; ENZYME ACTIVITY; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; HUMAN CELL; MAMMAL CELL; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DEGRADATION; UTERINE CERVIX CARCINOMA;

APOPTOSIS; BACTERIAL PROTEINS; CASPASE 3; CASPASES; COLORING AGENTS; DNA, COMPLEMENTARY; ENDOPEPTIDASES; ESCHERICHIA COLI; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; HELA CELLS; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LUMINESCENT PROTEINS; MICROSCOPY, FLUORESCENCE; POLYMERASE CHAIN REACTION; PROTEINS; RECOMBINANT PROTEINS; TIME FACTORS;

BACTERIA (MICROORGANISMS); MAMMALIA;

EID: 2442681401     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.04.147     Document Type: Article

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