Mycobacterial gene cloning and expression, comparative genomics, bioinformatics and proteomics in relation to the development of new vaccines and diagnostic reagents

Medical Principles and Practice

Volumn 14, Issue SUPPL. 1, 2005, Pages 27-34

  Mustafa, A S ^a  

^a KUWAIT UNIVERSITY   (Kuwait)

Author keywords

Antigens; Mycobacterium tuberculosis; Vaccine

Indexed keywords

ANTIGEN; BCG VACCINE; CROSS REACTING ANTIGEN; HEAT SHOCK PROTEIN; HYBRID PROTEIN; RECOMBINANT DNA; SYNTHETIC PEPTIDE;

BACTERIUM IDENTIFICATION; BIOINFORMATICS; CELLULAR IMMUNITY; COMPARATIVE GENOMIC HYBRIDIZATION; CONFERENCE PAPER; GENE EXPRESSION; MOLECULAR BIOLOGY; MOLECULAR CLONING; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PEPTIDE SYNTHESIS; POLYMERASE CHAIN REACTION; PROTEOMICS; SEQUENCE ANALYSIS; TUBERCULOSIS; VACCINATION;

ANTIGENS, BACTERIAL; BACTERIAL VACCINES; CLONING, MOLECULAR; COMPUTATIONAL BIOLOGY; GENE EXPRESSION REGULATION, BACTERIAL; GENETICS, MICROBIAL; HUMANS; MOLECULAR BIOLOGY; MYCOBACTERIUM TUBERCULOSIS; PROTEOMICS; REAGENT KITS, DIAGNOSTIC;

EID: 23844459522     PISSN: 10117571     EISSN: None     Source Type: Journal    
DOI: 10.1159/000086182     Document Type: Conference Paper

References (64)

1. Elimination of leprosy as a public health problem. http://www.who.int/ lep/.
2. Frieden TR, Sterling TR, Munsiff SS, Watt CJ, Dye C: Tuberculosis. Lancet 2003;362:887-899.
3. Fine PE: Variation in protection by BCG: Implications of and for heterologous immunity. Lancet 1995;346:1339-1345.
4. Schlossberg D: Tuberculosis and nontuberculous mycobacterial infections, ed 4. Philadelphia, WB Saunders Company, 1999, p 3.
5. Bloom BR, Mehra V, Young RA: Genes for the protein antigens of the tuberculosis and leprosy bacilli. Biosci Rep 1985;5:839-845.
6. Mustafa AS, Gill HK, Nerland A, Britton WJ, Mehra V, Bloom BR, Young RA, Godal T: Human T-cell clones recognize a major M. leprae protein antigen expressed in E. coli. Nature 1986;319:63-66.
7. Mustafa AS, Oftung F, Gill HK, Natvig I: Characteristics of human T-cell clones from BCG and killed M. leprae vaccinated subjects and tuberculosis patients. Recognition of recombinant mycobacterial antigens. Lepr Rev 1986;57(suppl 2):123-130.
8. Oftung F, Mustafa AS, Husson R, Young RA, Godal T: Human T cell clones recognize two abundant Mycobacterium tuberculosis protein antigens expressed in Escherichia coli. J Immunol 1987;138:927-931.
9. Mustafa AS: Identification of T-cell-activating recombinant antigens shared among three candidate antileprosy vaccines, killed M. leprae, M. bovis BCG, and mycobacterium w. Int J Lepr Other Mycobact Dis 1988;56:265-273.
10. Mustafa AS, Oftung F: Long-lasting T-cell reactivity to Mycobacterium leprae antigens in human volunteers vaccinated with killed M. leprae. Vaccine 1993;11:1108-1112.
11. Mustafa AS, Lundin KE, Oftung F: Human T cells recognize mycobacterial heat shock proteins in the context of multiple HLA-DR molecules: Studies with healthy subjects vaccinated with Mycobacterium bovis BCG and Mycobacterium leprae. Infect Immun 1993;61:5294-5301.
12. Mustafa AS, Lundin KE, Meloen RH, Shinnick TM, Oftung F: Identification of promiscuous epitopes from the mycobacterial 65-kilodalton heat shock protein recognized by human CD4(+) T cells of the Mycobacterium leprae memory repertoire. Infect Immun 1999;67:5683-5689.
13. Mustafa AS: HLA-restricted immune response to mycobacterial antigens: Relevance to vaccine design. Hum Immunol 2000;61:166-171.
14. Silva CL, Lowrie DB: A single mycobacterial protein (hsp 65) expressed by a transgenic antigen-presenting cell vaccinates mice against tuberculosis. Immunology 1994;82:244-248.
15. Silva CL, Silva MF, Pietro RC, Lowrie DB: Protection against tuberculosis by passive transfer with T-cell clones recognizing mycobacterial heat-shock protein 65. Immunology 1994;83:341-346.
16. Tascon RE, Colston MJ, Ragno S, Stavropoulos E, Gregory D, Lowrie DB: Vaccination against tuberculosis by DNA injection. Nat Med 1996;2:888-892.
17. Lowrie DB, Tascon RE, Bonato VL, Lima VM, Faccioli LH, Stavropoulos E, Colston MJ, Hewinson RG, Moelling K, Silva CL: Therapy of tuberculosis in mice by DNA vaccination. Nature 1999;400:269-271.
18. Mustafa AS, Oftung F, Deggerdal A, Gill HK, Young RA, Godal T: Gene isolation with human T lymphocyte probes. Isolation of a gene that expresses an epitope recognized by T cells specific for Mycobacterium bovis BCG and pathogenic mycobacteria. J Immunol 1988;141:2729-2733.
19. Oftung F, Wiker HG, Deggerdal A, Mustafa AS: A novel mycobacterial antigen relevant to cellular immunity belongs to a family of secreted lipoproteins. Scand J Immunol 1997;46:445-451.
20. Al-Attiyah R, Mustafa AS: Computer-assisted prediction of HLA-DR binding and experimental analysis for human promiscuous Th1-cell peptides in the 24 kDa secreted lipoprotein (LppX) of Mycobacterium tuberculosis. Scand J Immunol 2004;59:16-24.
21. Coler RN, Skeiky YA, Vedvick T, Bement T, Ovendale P, Campos-Neto A, Alderson MR, Reed SG: Molecular cloning and immunologic reactivity of a novel low molecular mass antigen of Mycobacterium tuberculosis. J Immunol 1998;16:2356-2364.
22. Dillon DC, Alderson MR, Day CH, Lewinsohn DM, Coler R, Bement T, Campos-Neto A, Skeiky YA, Orme IM, Roberts A, Steen S, Dalemans W, Badaro R, Reed SG: Molecular characterization and human T-cell responses to a member of a novel Mycobacterium tuberculosis mtb39 gene family. Infect Immun 1999;67:2941-2950.
23. Skeiky YA, Lodes MJ, Guderian JA, Mohamath R, Bement T, Alderson MR, Reed SG: Cloning, expression, and immunological evaluation of two putative secreted serine protease antigens of Mycobacterium tuberculosis. Infect Immun 1999;67:3998-4007.
24. Alderson MR, Bement T, Day CH, Zhu L, Molesh D, Skeiky YA, Coler R, Lewinsohn DM, Reed SG, Dillon DC: Expression cloning of an immunodominant family of Mycobacterium tuberculosis antigens using human CD4(+) T cells. J Exp Med 2000;191:551-560.
25. Reed S: TB vaccine development: Preclinical activities. Abstract session 5. Novel vaccines/vaccine strategies for TB. First Int Conf on TB Vaccines for the World, Montreal, September 2003.
26. Andersen P: Host responses and antigens involved in protective immunity to Mycobacterium tuberculosis. Scand J Immunol 1997;45:115-131.
27. Mustafa AS, Shaban FA, Abal AT, Al-Attiyah R, Wiker HG, Lundin KE, Oftung F, Huygen K: Identification and HLA restriction of naturally derived Th1-cell epitopes from the secreted Mycobacterium tuberculosis antigen 85B recognized by antigen-specific human CD4(+) T-cell lines. Infect Immun 2000;68:3933-3940.
28. Al-Attiyah R, Shaban FA, Wiker HG, Oftung F, Mustafa AS: Synthetic peptides identify promiscuous human Th1 cell epitopes of the secreted mycobacterial antigen MPB70. Infect Immun 2003;71:1953-960.
29. Andersen P, Andersen AB, Sorensen AL, Nagai S: Recall of long-lived immunity to Mycobacterium tuberculosis infection in mice. J Immunol 1995;154:3359-3372.
30. Mustafa AS, Amoudy HA, Wiker HG, Abal AT, Ravn P, Oftung F, Andersen P: Comparison of antigen-specific T-cell responses of tuberculosis patients using complex or single antigens of Mycobacterium tuberculosis. Scand J Immunol 1998;48:535-543.
31. Mustafa AS, Oftung F, Amoudy HA, Madi NM, Abal AT, Shaban F, Rosen Krands I, Andersen P: Multiple epitopes from the Mycobacterium tuberculosis ESAT-6 antigen are recognized by antigen-specific human T cell lines. Clin Infect Dis 2000;30(suppl 3):S201-S205.
32. Mustafa AS, Shaban FA, Al-Attiyah R, Abal AT, El-Shamy AM, Andersen P, Oftung F: Human Th1 cell lines recognize the Mycobacterium tuberculosis ESAT-6 antigen and its peptides in association with frequently expressed HLA class II molecules. Scand J Immunol 2003;57:125-134.
33. Skjot RL, Oettinger T, Rosenkrands I, Ravn P, Brock I, Jacobsen S, Andersen P: Comparative evaluation of low-molecular-mass proteins from Mycobacterium tuberculosis identifies members of the ESAT-6 family as immunodominant T-cell antigens. Infect Immun 2000;68:214-220.
34. Ravn P, Demissie A, Eguale T, Wondwosson H, Lein D, Amoudy HA, Mustafa AS, Jensen AK, Holm A, Rosenkrands I, Oftung F, Olobo J, von Reyn F, Andersen P: Human T cell responses to the ESAT-6 antigen from Mycobacterium tuberculosis. J Infect Dis 1999;179:637-645.
35. Olsen AW, Hansen PR, Holm A, Andersen P: Efficient protection against Mycobacterium tuberculosis by vaccination with a single subdominant epitope from the ESAT-6 antigen. Eur J Immunol 2000;30:1724-1732.
36. Al-Attiyah R, Mustafa AS, Abal AT, Madi NM, Andersen P: Restoration of mycobacterial antigen-induced proliferation and interferon-gamma responses in peripheral blood mononuclear cells of tuberculosis patients upon effective chemotherapy. FEMS Immunol Med Microbiol 2003;38:249-256.
37. Ewer K, Deeks J, Alvarez L, Bryant G, Waller S, Andersen P, Monk P, Lalvani A: Comparison of T-cell-based assay with tuberculin skin test for diagnosis of Mycobacterium tuberculosis infection in a school tuberculosis outbreak. Lancet 2003;361:1168-1173.
38. Berthet FX, Rasmussen PB, Rosenkrands I, Andersen P, Gicquel B: A Mycobacterium tuberculosis operon encoding ESAT-6 and a novel low-molecular-mass culture filtrate protein (CFP-10). Microbiology 1998;144:3195-3203.
39. Ahmad S, Amoudy HA, Thole JE, Young DB, Mustafa AS: Identification of a novel protein antigen encoded by a Mycobacterium tuberculosis-specific RD1 region gene. Scand J Immunol 1999;49:515-522.
40. Ahmad S, Ali MM, Mustafa AS: Construction of a modified vector for efficient purification of recombinant Mycobacterium tuberculosis proteins expressed in Escherichia coli. Protein Expr Purif 2003;29:167-175.
41. Mustafa AS, Al-Attiyah R: Tuberculosis: Looking beyond BCG vaccines. J Postgrad Med 2003;49:134-140.
42. Mustafa AS: Biotechnology in the development of new vaccines and diagnostic reagents against tuberculosis. Curr Pharm Biotechnol 2001;2:157-173.
43. Mustafa AS: Development of new vaccines and diagnostic reagents against tuberculosis. Mol Immunol 2002;39:113-119.
44. Mustafa AS: Progress towards the development of new anti-tuberculosis vaccines; in: Progress in Tuberculosis Research. Kuwait. New York, Nova Science Publishers, Inc., Editor: LC Smithe, 2005, p 47-76.
45. Mustafa AS, Cockle PJ, Shaban F, Hewinson RG, Vordermeier HM: Immunogenicity of Mycobacterium tuberculosis RD1 region gene products in infected cattle. Clin Exp Immunol 2002;130:37-42.
46. Cole ST, Brosch R, Parkhill J, Gamier T, Churcher C, Harris D, et al: Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 1998;393:537-544.
47. Behr MA, Wilson MA, Gill WP, Salamon H, Schoolnik GK, Rane S, Small PM: Comparative genomics of BCG vaccines by whole-genome DNA microarray. Science 1999;284:1520-1523.
48. Gordon SV, Brosch R, Billault A, Gamier T, Eiglmeier K, Cole ST: Identification of variable regions in the genomes of tubercle bacilli using bacterial artificial chromosome arrays. Mol Microbiol 1999;32:643-655.
49. Brosch R, Gordon SV, Pym A, Eiglmeier K, Gamier T, Cole ST: Comparative genomics of the mycobacteria. Int J Med Microbiol 2000;290:143-152.
50. Mustafa AS, Al-Attiyah R: Mycobacterium tuberculosis antigens and peptides as new vaccine candidates and immunodiagnostic reagents against tuberculosis. Kuwait Med J 2004;36:171-176.
51. Marmiesse M, Brodin P, Buchrieser C, Gutierrez C, Simoes N, Vincent V, Glaser P, Cole ST, Brosch R: Macro-array and bioinformatic analyses reveal mycobacterial 'core' genes, variation in the ESAT-6 gene family and new phylogenetic markers for the Mycobacterium tuberculosis complex. Microbiology 2004;150:483-496.
52. Liu XQ, Dosanjh D, Varia H, Ewer K, Cockle P, Pasvol G, Lalvani A: Evaluation of T-cell responses to novel RD1- and RD2-encoded Mycobacterium tuberculosis gene products for specific detection of human tuberculosis infection. Infect Immun 2004;72:2574-2581.
53. Demangel C, Brodin P, Cockle PJ, Brosch R, Majlessi L, Leclerc C, Cole ST: Cell envelope protein PPE68 contributes to Mycobacterium tuberculosis RD1 immunogenicity independently of a 10-kilodalton culture filtrate protein and ESAT-6. Infect Immun 2004;72:2170-2176.
54. Okkels LM, Brock I, Follmann F, Agger EM, Arend SM, Ottenhoff TH, Oftung F, Rosenkrands I, Andersen P: PPE protein (Rv3873) from DNA segment RD1 of Mycobacterium tuberculosis: Strong recognition of both specific T-cell epitopes and epitopes conserved within the PPE family. Infect Immun 2003;71:6116-6123.
55. Agger EM, Brock I, Okkels LM, Arend SM, Aagaard CS, Weldingh KN, Andersen P: Human T-cell responses to the RD1-encoded protein TB27.4 (Rv3878) from Mycobacterium tuberculosis. Immunology 2003;110:507-512.
56. Cockle PJ, Gordon SV, Lalvani A, Buddle BM, Hewinson RG, Vordermeier HM: Identification of novel Mycobacterium tuberculosis antigens with potential as diagnostic reagents or subunit vaccine candidates by comparative genomics. Infect Immun 2002;70:6996-7003.
57. Lewis KN, Liao R, Guinn KM, Hickey MJ, Smith S, Behr MA, Sherman DR: Deletion of RD1 from Mycobacterium tuberculosis mimics bacille Calmette-Guerin attenuation. J Infect Dis 2003;187:117-123.
58. Pym AS, Brodin P, Brosch R, Huerre M, Cole ST: Loss of RD1 contributed to the attenuation of the live tuberculosis vaccines Mycobacterium bovis BCG and Mycobacierium microti. Mol Microbiol 2002;46:709-717.
59. Pym AS, Brodin P, Majlessi L, Brosch R, Demangel C, Williams A, Griffiths KE, Marchal G, Leclerc C, Cole ST: Recombinant BCG exporting ESAT-6 confers enhanced protection against tuberculosis. Nat Med 2003;9:533-539.
60. Betts JC: Transcriptomics and proteomics: Tools for the identification of novel drug targets and vaccine candidates for tuberculosis. IUBMB Life 2002;53:239-242.
61. Jungblut PR, Muller EC, Mattow J, Kaufmann SH: Proteomics reveals open reading frames in Mycobacterium tuberculosis H37Rv not predicted by genomics. Infect Immun 2001;69:5905-5907.
62. Dobos KM, Spencer JS, Orme IM, Belisle JT: Proteomic approaches to antigen discovery. Methods Mol Med 2004;94:3-17.
63. Covert BA, Spencer JS, Orme IM, Belisle JT: The application of proteomics in defining the T cell antigens of Mycobacterium tuberculosis. Proteomics 2001;1:574-586.
64. Mattow J, Schaible UE, Schmidt F, Hagens K, Siejak F, Brestrich G, Haeselbarth G, Muller EC, Jungblut PR, Kaufmann SH: Comparative proteome analysis of culture supernatant proteins from virulent Mycobacterium tuberculosis H37Rv and attenuated M. bovis BCG Copenhagen. Electrophoresis 2003;24:3405-3420.