Use of cell wall-less bacteria (L-forms) for efficient expression and secretion of heterologous gene products

Current Opinion in Biotechnology

Volumn 9, Issue 5, 1998, Pages 506-509

  Gumpert, Johannes ^a   Hoischen, Christian ^a  

^a FRITZ LIPMANN INSTITUTE   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PROTEINASE; RECOMBINANT PROTEIN;

BACTERIAL CELL WALL; CELL INCLUSION; CELL MEMBRANE; DEGRADATION; ESCHERICHIA COLI; GENE EXPRESSION SYSTEM; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN SYNTHESIS; PROTEUS MIRABILIS; PROTOPLAST; SHORT SURVEY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; MIRABILIS; PROKARYOTA; PROTEUS MIRABILIS;

EID: 0032190666     PISSN: 09581669     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0958-1669(98)80037-2     Document Type: Article

References (19)

1. Madoff S. The Bacterial L-Forms. 1986;Marcel Dekker Inc, New York.
2. Mattmann LH. Cell Wall Deficient Forms. 1993;CRC Press, Boca Raton.
3. Gumpert J, Taubeneck U. Characteristic properties and biological significance of stable protoplast type L-forms. Protoplasts, Lecture Proceedings of the 6th International Protoplast Symposium: Basel. Experientia. 46:1983;227-241. (suppl).
4. Gumpert J. Celluar growth without a murein sacculus - the nucleoid-associated compartmentation concept. De Pedro MA, Höltje V, Löffelhardt W. Bacterial Growth and Lysis: Metabolism and Structure of the Bacterial Sacculus. 1993;459-463 Plenum Publishing, New York.
5. Taubeneck U. Untersuchungen über die L-Form von Proteus mirabilis, Hauser II Entwicklung und Wesen der L-Form. Z All Mikrobiol. 2:1962;132-156. [Title translation: Studies of the L-form of Proteus mirabilis Hauser, II: Development and nature of the L-form.].
6. +.].
7. Gura K. Charakterisierung der Membranlipide aus stabilen Protoplastentyp L-Formen und N-Formen von Escherichia coli, Proteus mirabilis und Streptomyces hygroscopicus. PhD Thesis. 1998;University of Halle-Wittenberg, Germany. [Title translation: Characterisation of the membrane lipids from the stable protoplast type L-form and N-form of Escherichia coli, Proteus miribalis and Streptomyces hygroscopicus.].
8. Hoischen C, Gura K, Luge C, Gumpert J. Lipid and fatty acid composition of cytoplasmic membranes from Streptomyces hygroscopicus and its stable protoplast type L-form. J Bacteriol. 179:1997;3430-3436.
9. Klessen CH, Schmidt KH, Gumpert J, Grosse HH, Malke H. Complete secretion of activable bovine prochymosin by genetically engineered L-forms of Proteus mirabilis. Appl Environ Microbiol. 55:1989;1009-1015.
10. Laplace F, Müller J, Gumpert J, Malke H. Novel shuttle vectors for improved streptokinase expression in streptococci and bacterial L-forms. FEMS Microbiol Lett. 65:1989;89-94.
11. Laplace F, Egerer R, Gumpert J, Kraft R, Kostka R, Malke H. Heterologous signal peptide processing in fusion interferon synthesis by engineered L-forms of Proteus mirabilis. FEMS Microbiol Lett. 59:1989;59-64.
12. Gumpert J, Cron H, Plapp R, Niersbach H, Hoischen C. Synthesis and secretion of recombinant penicillin G acylase in bacterial L-forms. J Basic Microbiol. 36:1996;89-98.
13. Kujau M, Hoischen C, Riesenberg D, Gumpert J. Expression and secretion of functional miniantibodies McPC603scFvDhlx in cell wall-less L-form strains of Proteus mirabilis and Escherichia coli: a comparison of the synthesis capacities of L-form strains with an E. coli producer strain. of outstanding interest Appl Microbiol Biotechnol. 49:1998;51-58 The results show that L-form cells of P. mirabilis have a similar high-synthesis capacity for a miniantibody protein as the producer strain E. coli RV308 and that the portion of the active antibody can be improved by lowering the growth temperature and by special supplements. They demonstrate further that a periplasmic compartment is not a prerequisite for the correct folding and modification of the miniantibody molecules.
14. Sieben S. Die stabilen Protoplasten-Typ L-Formen von Proteus mirabilis als neues Expressionssystem für sekretorische proteine und integrale Membranproteine. PhD Thesis. 1998;University of Jena, Germany. [Title translation: Stable protoplast-type L-forms of Proteus mirabilis represent a new expression system for secretory and integral membrane proteins.].
15. Schlott B, Hartmann M, Gührs KH, Birch-Hirschfeld E, Pohl HD, Vanderschueren S, van de Werf F, Michoel A, Collen D, Behnke D. High yield production and purification of recombinant staphylokinase for thrombolytic therapy. Biotechnology. 12:1994;185-189.
16. Pack P, Kujau M, Schroekh V, Knüpfer U, Wenderoth R, Riesenberg D, Plückthun A. Improved bivalent miniantibodies, with identical avidity as whole antibodies, produced by high cell density fermentation of Escherichia coli. Biotechnology. 11:1993;1271-1277.
17. Plückthun A. Antibodies from Escherichia coli. Rosenberg M, Moore GP. Handbook of Experimental Pharmacology 113. 1994;269-315 Springer, Berlin.
18. Schumacher G, Sizmann D, Haug H, Buckel P, Böck A. Penicillin acylase from E. coli: unique gene-protein relation. Nucleic Acids Res. 14:1986;5713-5728.
19. Bushueva AM, Shevelev AB, Gumpert J, Chestukhina GG, Serkina AV, Hoischen C, Matz MV, Kuryatova MV, Stepanov VM. Expression of the carboxypeptidase T gene from Thermoactinomyces vulgaris in stable protoplast type L-forms of Proteus mirabilis. FEMS Microbiol Lett. 159:1998;145-150.