Enhancing recombinant protein yields in Escherichia coli using the T7 system under the control of heat inducible λP(L) promoter

Journal of Biotechnology

Volumn 68, Issue 2-3, 1999, Pages 125-134

  Gupta, Jagdish C ^a   Jaisani, Manish ^a   Pandey, Gaurav ^a   Mukherjee, K J ^a  

^a JAWAHARLAL NEHRU UNIVERSITY   (India)

Author keywords

galactosidase; P(L) promoter; E. coli; Fed batch; Heat shock; Overexpression; Recombinant; T7 promoter

Indexed keywords

DENSITY (SPECIFIC GRAVITY); ENZYMES; ESCHERICHIA COLI; RNA;

HEAT SHOCK;

GENETIC ENGINEERING;

BETA GALACTOSIDASE; DNA DIRECTED RNA POLYMERASE; RECOMBINANT PROTEIN;

ARTICLE; CELL DENSITY; DRUG MANUFACTURE; ESCHERICHIA COLI; GENE EXPRESSION; GENE TRANSFER; HEAT SHOCK; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; TECHNIQUE;

BACTERIOPHAGE T7; BETA-GALACTOSIDASE; CULTURE MEDIA; DNA-DIRECTED RNA POLYMERASES; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; HEAT; HEAT-SHOCK RESPONSE; LAC OPERON; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 0032993771     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(98)00193-X     Document Type: Article

References (22)

1. Chamberlin M., McGrath J., Waskell L. New RNA polymerase from Escherichia coli infected with bacteriophage T7. Nature (London). 228:1970;227-237.
2. Gourse R.L., Takebe Y., Sharrock R.A., Nomura M. Feedback regulation of rRNA and tRNA synthesis and accumulation of free ribosomes after conditional expression of rRNA genes. Proc. Natl. Acad. Sci. USA. 82:1985;1069-1073.
3. Hui A., Jhurani P., de Boer H.A. Directing ribosomes to a single mRNA species: a method to study ribosomal RNA mutations and their effects on translation of a single messenger in Escherichia coli. Methods Enzymol. 153:1987;432-452.
4. Jensen E.B., Carlsen S. Production of recombinant human growth hormone in Escherichia coli: expression of different precursors and physiological effects of glucose, acetate and salts. Biotechnol. Bioeng. 36:1990;1-11.
5. Kim J.Y., Ryu D.D.Y. The effects of plasmid content, transcription efficiency on the productivity of a cloned gene protein in Escherichia coli. Biotechnol. Bioeng. 38:1991;1271-1279.
6. Kwon S., Kim S., Kim E. Effects of glycerol on β-lactamase production during high cell density cultivation of recombinant Escherichia coli. Biotechnol. Prog. 12:1996;205-208.
7. Lee J., Ramirez W.F. Mathematical modeling of induced foreign protein production by recombinant bacteria. Biotechnol. Bioeng. 39:1992;635-646.
8. Miller J.H. Assay of β-galactosidase. Experiments in Molecular Genetics. 1972;352-355 Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
9. Okita B., Arcuri E., Turner K., Sharr D., Del Tito B., Swanson J., Shatzman A., Zabriskie D. Effect of induction temperature on the production of malaria antigens in recombinant E. coli. Biotechnol. Bioeng. 34:1989;854-862.
10. L promoter of Coliphage Lambda. Gene. 15:1981;59-76.
11. Remaut E., Stanssens P., Fritsch W. Inducible high level synthesis of mature human fibroblast interferon in Escherichia coli. Nucleic Acids Res. 11:1983;4677-4688.
12. Sakamoto S., Iijima M., Matsuzawa H., Ohta T. Production of thermophilic protease by glucose-controlled fed-batch culture of recombinant Escherichia coli. J. Ferment. Bioeng. 78:1994;304-309.
13. Sambrook J., Maniatis T., Fritsch E.F. Molecular Cloning: A Laboratory Manual. 2nd edition:1989;Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
14. Seo J.H., Bailey J.E. Effects of recombinant plasmid on growth properties and cloned gene product formation in Escherichia coli. Biotechnol. Bioeng. 27:1985;1668-1674.
15. Shatzmant A.R., Rosenberg M. Efficient expression of heterologous genes in Escherichia coli. Ann. NY Acad. Sci. 428:1986;233-248.
16. Shiloach J., Baner S. High yield growth of E. coli at different temperatures in a bench scale fermentor. Biotechnol. Bioeng. 17:1975;227-239.
17. Shimizu M., Iijima S., Kobayashi T. Production of insecticidal protein of Bacillus thuringiensis by cultivation of recombinant Escherichia coli. J. Ferment. Bioeng. 74:1992;163-168.
18. Studier F.W., Rosenberg A.H., Dunn J.J., Dubendorff J.W. Use of T7 polymerase to direct expression of cloned genes. Methods Enzymol. 185:1990;60-89.
19. Studier W., Moffait B.A. Use of bacteriophage T7 RNA polymerase to direct selective high level expression of specific genes. J. Mol. Biol. 189:1986;115-130.
20. Tabor S., Richardson C.C. A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes. Proc. Natl. Acad. Sci. USA. 82:1985;1074-1078.
21. Yee L., Blanch H.W. Defined media optimization for growth of recombinant Escherichia coli X90. Biotechnol. Bioeng. 41:1993a;221-230.
22. Yee L., Blanch H.W. Recombinant trypsin production in high cell density fed-batch cultures in Escherichia coli. Biotechnol. Bioeng. 44:1993b;819-829.