Effect of zwf gene knockout on the metabolism of Escherichia coli grown on glucose or acetate

Metabolic Engineering

Volumn 6, Issue 2, 2004, Pages 164-174

  Zhao, Jiao ^a   Baba, Tomoya ^a   Mori, Hirotada ^a   Shimizu, Kazuyuki ^a,b  

^a KEIO UNIVERSITY   (Japan)

^b KYUSHU INSTITUTE OF TECHNOLOGY   (Japan)

Author keywords

Acetate; Escherichia coli; Glucose; Knockout; Oxidative pentose phosphate pathway; Zwf gene

Indexed keywords

ESCHERICHIA COLI;

EID: 2342565059     PISSN: 10967176     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymben.2004.02.004     Document Type: Article

References (28)

1. Canonaco, F., Hess, T.A., Heri, S., Wang, T., Szyperski, T., Sauer, U., 2001. Metabolic flux response to phosphoglucose isomerase knockout in Escherichia coli and impact of overexpression of the soluble transhydrogenase Udha. FEMS Microbiol. Lett. 204, 247-252.
2. Colowick, S.P., 1963. Preparation and assay of enzymes. Methods Enzymol. 6, 1-640.
3. Datsenko, K.A., Wanner, B.L., 2000. One-step inactivation of chromosomal genes in Escherichia coli K12 using PCR products. Proc. Natl. Acad. Sci. USA. 97, 6640-6645.
4. Dauner, M., Bailey, J.E., Sauer, U., 2001. Metabolic flux analysis with a comprehensive isotopomer model in Bacillus subtilis. Biotechnol. Bioeng. 76, 144-156.
5. Emmerling, M., Dauner, M., Ponti, A., Fiaux, J., Hochuli, M., Szyperski, T., Wuthrich, K., Bailey, J.E., Sauer, U., 2002. Metabolic flux responses to pyruvate kinase knockout in Escherichia coli. J. Bacteriol. 184, 152-164.
6. Fraenkel, D.G., 1968. Selection of mutants lacking glucose-6-phosphate dehydrogenase or gluconate-6-phosphate dehydrogenase. J. Bacteriol. 95, 1267-1271.
7. Genshi, Z., Winkler, M.E., 1994. An Escherichia coli K12- tktA tktB mutant deficient in transketolase activity requires pyridoxine (vitamine B6) as well as the aromatic amino acids and vitamins for growth. J. Bacteriol. 176, 6134-6138.
8. Holger, J., Bernhard, K., Peter, K., Karl-Dieter, E., 1996. Mutants that show increased sensitivity to hydrogen peroxide reveal an important role for the pentose phosphate pathway in protection of yeast against oxidative stress. Mol. Gen. Genet. 252, 456-464.
9. Holms, H., 1996. Flux analysis and control of the central metabolic pathways in Escherichia coli. FEMS Microbiol. Rev. 19, 85-116.
10. Josephson, B.L., Fraenkel, D.G., 1969. Transketolase mutants of Escherichia coli. J Bacteriol. 100, 1289-1295.
11. Josephson, B.L., Fraenkel, D.G., 1974. Sugar metabolism in transketolase mutants of Escherichia coli. J. Bacteriol. 118, 1082-1089.
12. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193, 265-275.
13. Marx, A., Graaf, A.A., Wiechert, W., Eggeling, L., Sahm, H., 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spectroscopy combined with metabolite balancing. Biotechnol. Bioeng. 49, 111-129.
14. Möllney, M., Wiechert, W., Kownatzki, D., Graaf, A.A., 1999. Bidirectional reaction steps in metabolic networks: IV. Optimal design of isotopomer labelling experiments. Biotechnol. Bioeng. 66, 86-103.
15. Neidhardt, FC., Ingraham, JL., Schaechter, M., 1990. Physiology of the Bacterial Cell: A Molecular Approach. Sinauer Associate, Sunderland Inc., MA.
16. Oh, M.K., Rohlin, L., Kao, K.C., Liao, J.C., 2002. Global expression profiling of acetate-grown Escherichia coli. J. Biol. Chem. 277, 13175-13183.
17. Park, S.M., Sinskey, A.J., stephanopoulos, G., 1997. Metabolic and physiological studies of Corynebacterium glutamicum mutant. Biotechnol. Bioeng. 55, 864-878.
18. Paul Lee, W.N., 1991. Mass isotopomer analysis: theoretical and practical consideration. Biol. Mass Spectrometry 20, 451-458.
19. Sauer, U., Lasko, D.R., Fiaux, J., Hochuli, M., Glaser, R., Szyperski, K.W., Bailey, J.E., 1999. Metabolic flux ratio analyis of genetic and environmental modulations of Escherichia coli central carbon metabolism. J. Bacteriol. 181, 6679-6688.
20. Schmidt, K., Nielsen, J., Villadsen, J., 1997. Modeling isotopomer distributions in metabolic networks using isotopomer mapping matrices. Biotechnol. Bioeng. 55, 831-840.
21. Schmidt, K., Nielsen, J., Villadsen, J., 1999. Quantitative analysis of metabolic fluxes in Eshcerichia coli using two-dimensional NMR spectroscopy and complete isotopomer models. J. Biotechnol. 71, 175-190.
22. Sprenger, G.A., 1995. Genetics of pentose-phosphate pathway enzymes of Escherichia coli K12. Arch. Microbiol. 164, 324-330.
23. 13C-labeling of proteinogenic amino acids - an efficient analytical tool to investigate intermediary metabolism. Eur. J. Biochem. 232, 433-448.
24. Walsh, K., Koshland Jr, D.E., 1985. Branch point control by the phosphorylation state of isocitrate dehydrogenase. J. Biol. Chem. 260, 8430-8437.
25. Wiechert, W., Siefke, C., Graaf, A.A., Marx, A., 1997. Biodirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis. Biotechnol. Bioeng. 55, 118-135.
26. Winzeler, E., Shoemaker, D., Astromoff, A., Liang, H., Anderson, K., Andre, B., Bangham, R., Benito, R., Boeke, J., Bussey, H., Chu, A., Connelly, C., Davis, K., Dietrich, F., Dow, S., El, B., Foury, M.F., Friend, S., Gentalen, E., Giaever, G., Hegemann, J., Jones, T., Laub, M., Liao, H., Liebundguth, N., Lockhart, D., Lucau-Danila, A., Lussier, M., M'Rabet, N., Menard, P., Mittmann, M., Pai, C., Rebischung, C., Revuelta, J., Riles, L., Roberts, C., Ross-MacDonald, P., Scherens, B., Snyder, M., Sookhai-Mhadeo, S., Storms, R., Veronneau, S., Voet, M., Volckaert, G., Ward, T., Wysocki, R., Yen, G., Yu, K., Zimmermann, K., Philippsen, P., Johnston, M., Davis, R., 1999. functional characterization of the Saccharomyces cervisiae genome by precise deletion and parallel analysis. Science 285, 901-906.
27. Wittmann, C., Heinzle, E., 1999. Mass spectrometry for metabolic flux analysis. Biotechnol. Bioeng. 62, 739-750.
28. Zhao, J., Shimizu, K., 2003. Metabolic flux analysis of Escherichia coli K12 grown labeled acetate and glucose using GC-MS and powerful flux calculation method. J. Biotechnol. 101 (2), 101-117.