Metabolic and physiological studies of Corynebacterium glutamicum mutants

Biotechnology and Bioengineering

Volumn 55, Issue 6, 1997, Pages 864-879

  Park, S M ^a   Sinskey, A J ^b   Stephanopoulos, Gregory ^a  

^a USA   (United States)

^b MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

Corynebacterium glutamicum mutants; Intracellular flux analysis; Transconjugation

Indexed keywords

ADENOSINETRIPHOSPHATE; ALDEHYDES; BACTERIA; BIOASSAY; BIOSYNTHESIS; CATALYST ACTIVITY; FERMENTATION; GENES; GLUCOSE; GROWTH KINETICS; METABOLISM; REDUCTION;

CORYNEBACTERIUM GLUTAMICUM MUTANTS; GENE TRANSCONJUGATION; INTRACELLULAR FLUX ANALYSIS;

GENETIC ENGINEERING;

ADENOSINE TRIPHOSPHATE; BACTERIAL ENZYME; CARBON 13; CARBON DIOXIDE; GLUCOSE; NICOTINAMIDE ADENINE DINUCLEOTIDE (PHOSPHATE) TRANSHYDROGENASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; TRICARBOXYLIC ACID;

ARTICLE; BACTERIAL METABOLISM; BACTERIUM MUTANT; CARBON DIOXIDE FIXATION; CATALYSIS; CORYNEBACTERIUM GLUTAMICUM; ENERGY METABOLISM; ENZYME ACTIVITY; FERMENTATION; NONHUMAN;

EID: 0343196707     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19970920)55:6<864::AID-BIT5>3.0.CO;2-E     Document Type: Article

References (26)

1. Aiba, S., Matsuoka, M. 1979. Identification of metabolic model: citrate production from glucose by Candida lipolytica. Biotechnol Bioeng. 21: 1373-1386.
2. Boles, E., Lehnert, W., Zimmermann, F. K. 1993. The role of the NAD-dependent dehydrogenase in restoring growth on glucose of a Saccharomyces cerevisiae phosphoglucose isomerase mutant. Eur. J. Biochem. 217: 469-477.
3. Cortassa, S., Aon, J. C. Aon, M. A. 1995. Fluxes of carbon, phosphorylation, and redox intermediates during growth of Saccharomyces cerevisiae on different carbon sources. Biotechnol. Bioeng. 47: 193-208.
4. Diaz-Ricci, J. C., Tsu, M., Bailey, J. E. 1992. Influence of expression of the pet operon on intracellular metabolic fluxes of Escherichia coli. Biotechnol. Bioeng. 39: 59-65.
5. Gubler, M., Park, S. M. Jetten, M., Stephanopoulos, G., Sinskey, A. J. 1994a. Effects of phosphoenol pyruvate carboxylase deficiency on metabolism and lysine production in Corynebacterium glutamicum. Appli. Microbiol. Biotechnol. 40: 857-863.
6. Gubler, M., Jetten, M., Lee, S. H., Sinskey, A. J. 1994b. Cloning of the pyruvate kinase gene (pyk) of Corynebacterium glutamicum and site-specific inactivation of pyk in a lysine producing Corynebacterium lactofermentum strain. Appl. Microbiol. Biotechnol. 60: 2494-2500.
7. Hoek, J. B., Rydström, J. 1988. Physiological roles of nicotinamide nucleotide transhydrogenase Biochem. J. 254: 1-10.
8. Jørgensen, H., Nielsen, J., Villadsen, J., Mollgaard, H. 1995. Metabolic flux distributions in Penicillium chrysogenum during fed-batch cultivations. Biotechnol. Bioeng. 46: 117-131.
9. Kiss, R. D., Stephanopoulos, G. 1991. Metabolic activity control of the L-lysine fermentation by restrained growth fed-batch strategies. Biotechnol. Prog. 7: 501-509.
10. Mori, M., Shiio, I. 1985. Purification and some properties of phosphoenol pyruvate carboxylase from Brevibacterium flavum and its over producing mutant. J. Biochem. 97: 1119-1128.
11. Neidhardt, F. C., Ingraham, J. L., Schaechter, M. 1990. Physiology of the Bacterial Cell. Sinauer Associates, Inc., Sunderland, MA.
12. Papoutsakis, E. T. 1984. Equations and calculations for fermentations of butyric acid bacteria. Biotechnol Bioeng. 26: 174-187.
13. Papoutsakis, E. T., Meyer, C. L. 1985a. Fermentation equations for propionic-acid bacteria and production of assorted oxychemicals from various sugars. Biotechnol. Bioeng. 27: 67-80.
14. Papoutsakis, E. T., Meyer, C. L. 1985b. Equations and calculations of product yields and preferred pathways for butanediol and mixed-acid fermentations. Biotechnol. Bioeng. 27: 50-66.
15. 13C-NMR spectroscopy and GC-MS. Appl. Microbiol. Biotechnol. 47: 430-440.
16. Phelps, D. C., Hatefi, Y. 1981. Inhibition of the mitochondrial nicotin-amide nucleotide transhydrogenase by dicyclohexylcarbodiimide and diethylpyrocarbonate. J. Biol. Chem. 256: 8217-8221.
17. Reardon, K. F., Scheper, T. H., Bailey, J. E. 1987. Metabolic pathway rates and culture fluorescence in batch fermentations of Clostridium acetobutylicum. Biotechnol. Prog. 3: 153-167.
18. Sambrook, J., Fritsch, E. F., Maniatis, T. 1989. Molecular cloning-a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
19. Vallino, J. J., Stephanopoulos G. 1987. Intelligent sensors in biotechnology applications for the monitoring of fermentations and cellular metabolism. Annals N.Y. Acad. Sci. 506: 415-430.
20. Vallino, J. J., Stephanopoulos, G. 1990. Flux determination in cellular bioreaction networks. Application to Lysine Fermentations. Frontiers in Bioprocessing, S. Sikdar, M. Bier, P. Todd (eds.), pp. 205-219, CRC Press, Inc., Boca Raton, FL.
21. Vallino, J. J., Stephanopoulos, G. 1993. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction. Biotechnol. Bioeng. 41: 633-646.
22. Vallino, J. J., Stephanopoulos, G. 1994a. Carbon flux distributions at the glucose-6-phosphate branch point in Corynebacterium glutamicum during lysine overproduction. Biotechnol. Prog. 10: 327-334.
23. Vallino, J. J. and Stephanopoulos, G. 1994b. Carbon flux distributions at the pyruvate branch point in C. glutamicum during lysine overproduction. Biotechnology Progress 10: 327-334.
24. Wang, N. S., Stephanopoulos, G. 1983. Application of macroscopic balances to the identification of gross measurement errors. Biotechnol Bioeng. 25: 2177-2208.
25. Zahl, K. J., Rose, C., Hanson, R. L. (1978). Isolation and partial characterization of a mutant of Escherichia coli lacking pyridine nucleotide transhydrogenase. Arch. Biochem. Biophys. 190: 598-602.
26. 13C NMR. Biotechnol. Bioeng. 45: 292-303.