Metabolic flux analysis with a comprehensive isotopomer model in Bacillus subtilis

Biotechnology and Bioengineering

Volumn 76, Issue 2, 2001, Pages 144-156

  Wiebe, Marilyn G ^a   Karandikar, Atul ^a   Robson, Geoff D ^a   Trinci, Anthony P J ^a   Candia, Juana L Flores ^b   Trappe, Susanne ^b   Wallis, Gregg ^b   Rinas, Ursula ^b   Derkx, Patrick M F ^c   Madrid, Susan M ^c   Sisniega, Heidi ^d   Faus, Ignacio ^d   Montijn, Roy ^e   Van Den Hondel, Cees A M J J ^e   Punt, Peter J ^e  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b HELMHOLTZ CENTRE FOR INFECTION RESEARCH   (Germany)

^c DANISCO A S   (Denmark)

^d Department of Biotechnology   (Spain)

^e TNO   (Netherlands)

Author keywords

Bacillus subtilis; Central metabolism; Isotopomer model; Metabolic flux analysis; NMR spectroscopy

Indexed keywords

CHEMOSTATS; GENETIC ENGINEERING; GLUCOSE; METABOLISM; NUCLEAR MAGNETIC RESONANCE;

ISOTOPOMER MODEL;

BIOTECHNOLOGY;

AMINO ACID; CARBON 13; GLUCOSE; MALIC ACID; PHOSPHOPYRUVATE CARBOXYLASE; RIBOFLAVIN;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIUM CULTURE; BIOENGINEERING; BIOMASS; CARBON DIOXIDE FIXATION; CARBON NUCLEAR MAGNETIC RESONANCE; CHEMICAL COMPOSITION; CHEMOSTAT; CITRIC ACID CYCLE; CONTROLLED STUDY; ENZYME MECHANISM; EXPERIMENTAL MODEL; GLUCONEOGENESIS; GLYCOLYSIS; NONHUMAN; PENTOSE PHOSPHATE CYCLE; PREDICTION; PROTON NUCLEAR MAGNETIC RESONANCE; RELIABILITY; STATISTICAL ANALYSIS; VALIDATION PROCESS;

AMINO ACIDS; BACILLUS SUBTILIS; BIOMASS; BIOREACTORS; CARBON; CARBON ISOTOPES; CITRIC ACID CYCLE; GENETIC ENGINEERING; GLUCONEOGENESIS; GLUCOSE; GLYCOLYSIS; KINETICS; MAGNETIC RESONANCE SPECTROSCOPY; MODELS, BIOLOGICAL; MODELS, STATISTICAL; PREDICTIVE VALUE OF TESTS; RIBOFLAVIN; SENSITIVITY AND SPECIFICITY; STATISTICS;

BACILLUS SUBTILIS;

EID: 0034847930     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.1154     Document Type: Article

References (43)

1. An overview of evolutionary algorithms for parameter optimization
2. Metabolic flux analysis of hybridoma cells in different culture media using mass balances
3. Flux analysis of underdetermined metabolic networks: The quest for the missing constraints
4. Isotopomer analysis using GC-MS
5. GC-MS analysis of amino acids rapidly provides rich information for isotopomer balancing
6. Stoichiometric growth model for riboflavin-producing Bacillus subtilis
7. Role of pyruvate carboxylase, phosphoenolpyruvate carboxykinase, and malic enzyme during growth and sporulation of Bacillus subtilis
8. Effect of reversible reactions on isotope label redistribution - Analysis of the pentose phosphate pathway
9. Experimentelle aspekte der NMR-spektroskopie
10. Eco Cyc: Encyclopedia of Escherichia coli genes and metabolism
11. The use of glucose-C-14 for the evaluation of the pathways of glucose metabolism
12. 13C] glucose
13. Optimization by simulated annealing
14. 2 data, with applications to peripheral ganglia of chicken embryos
15. Determination of the fluxes in the central metabolism of Corynebacterium by nuclear magnetic resonance spectroscopy combined with metabolite balancing
16. Response of the central metabolism in Corynebacterium glutamicum to the use of an NADH-dependent glutamate dehydrogenase
17. Carbon isotope effects on the pyruvate dehydrogenase reaction and their importance for relative carbon-13 depletion in lipids
18. Bidirectional reaction steps in metabolic networks: IV. Optimal design of isotopomer labeling experiments
19. A simplex method for function minimization
20. Genetic engineering of Bacillus subtilis for the commercial production of riboflavin
21. In vivo quantification of parallel and bidirectional fluxes in the anaplerosis of Corynebacterium glutamicum
22. Metabolic fluxes in riboflavin-producing Bacillus subtilis
23. Physiology and metabolic fluxes of wild-type and riboflavin-producing Bacillus subtilis
24. Automated sampling device for monitoring intracellular metabolite dynamics
25. Modeling isotopomer distributions biochemical networks using isotopomer mapping matrices
26. Quantitative analysis of metabolic fluxes in Escherichia coli, using two-dimensional NMR spectroscopy and complete isotopomer models
27. 13C coupling constraints on the isotopomer distribution in labeled biomass components
28. 13C-labeling of proteinogenic amino acids. An efficient analytical tool to investigate intermediary metabolism
29. 13C-NMR MS, and metabolic flux balancing in biotechnology research
30. 13C labeling and two-dimensional NMR spectroscopy
31. Metabolic flux distributions in Corynebacterium glutamicum during growth and lysine overproduction
32. Linear constraint relations in biochemical reaction systems: I. Classification of the calculability and the balanceability of conversion rates
33. Metabolic flux balancing: Basic concepts, scientific and practical use
34. In vivo dynamics of the pentose phosphate pathway in Saccharomeyces cerevisiae
35. Application of macroscopic balances to the identification of gross measurement errors
36. Carbon isotope discrimination in a plant possessing the C4 dicarboxylic acid pathway
37. Bidirectional reaction steps in metabolic networks: I. Modeling and simulation of carbon isotope labelling experiments
38. Bidirectional reaction steps in metabolic networks: II. Flux estimation and statistical analysis
39. Numerical isotopomer analysis: Estimation of metabolic activity