Increasing the molecular contacts between maurotoxin and Kv1.2 channel augments ligand affinity

Proteins: Structure, Function and Genetics

Volumn 60, Issue 3, 2005, Pages 401-411

  M'Barek, Sarrah ^a   Chagot, Benjamin ^b   Andreotti, Nicolas ^a,c   Visan, Violeta ^d   Mansuelle, Pascal ^a   Grissmer, Stephan ^d   Marrakchi, Mohamed ^e   El Ayeb, Mohamed ^f   Sampieri, François ^a   Darbon, Hervé ^b   Fajloun, Ziad ^c   De Waard, Michel ^g   Sabatier, Jean Marc ^a,c  

^a CNRS   (France)

^b Centre National de la Recherche Scientifique   (France)

^c Laboratoire Cellpep SA   (France)

^d UNIVERSITY OF ULM   (Germany)

^e FACULTÉ DES SCIENCES DE TUNIS   (Tunisia)

^f PASTEUR INSTITUTE OF TUNIS   (Tunisia)

^g CEA GRENOBLE   (France)

Author keywords

Butantoxin; Chimera toxin; K+ channels; Maurotoxin; Molecular contacts; Scorpion toxin; Toxin affinity

Indexed keywords

BUTANTOXIN; CYSTINE; MAUROTOXIN; POTASSIUM CHANNEL; POTASSIUM ION; SCORPION VENOM; UNCLASSIFIED DRUG;

ARTICLE; BINDING AFFINITY; CHIMERA; CIRCULAR DICHROISM; COMPLEX FORMATION; DISULFIDE BOND; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LIGAND BINDING; MOLECULAR INTERACTION; MOLECULAR MODEL; NONHUMAN; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; STRUCTURE ANALYSIS; THREE DIMENSIONAL IMAGING; TOXIN ANALYSIS;

SCORPIONES;

EID: 23044500282     PISSN: 08873585     EISSN: None     Source Type: Journal    
DOI: 10.1002/prot.20509     Document Type: Article

References (38)

1. Kharrat R, Mansuelle P, Sampieri F, Crest M, Oughideni R, Van Rietschoten J, Martin-Eauclaire M-F, Rochat H, El Ayeb M. Maurotoxin, a four disulfide bridge toxin from Scorpio maurus venom: purification, structure and action on potassium channels. FEBS Lett 1997;406:284-290.
2. Kharrat R, Mabrouk K, Crest M, Darbon H, Oughideni R, Martin-Eauclaire MF, Jacquet G, El Ayeb M, Van Rietschoten J, Rochat H, Sabatier JM. Chemical synthesis and characterization of maurotoxin, a short scorpion toxin with four disulfide bridges that acts on K+ channels. Eur J Biochem 1996;242:491-498.
3. Fajloun Z, Ferrat G, Carlier E, Fathallah M, Lecomte C, Sandoz G, di Luccio E, Mabrouk K, Legros C, Darbon H, and others. Synthesis, 1H NMR structure, and activity of a three-disulfide-bridged maurotoxin analog designed to restore the consensus motif of scorpion toxins. J Biol Chem 2000;275:13605-13612.
4. 2+-activated potassium channels. Mol Pharmacol 2003;63:409-418.
5. Fajloun Z, Mosbah A, Carlier E, Mansuelle P, Sandoz G, Fathallah M, di Luccio E, Devaux C, Rochat H, De Waard M, and others. Maurotoxin versus Pi1/HsTx1 scorpion toxins. Toward new insights in the understanding of their distinct disulfide bridge patterns. J Biol Chem 2000;15:39394-39402.
6. + channels. J Biol Chem 2003;278:31095-31104.
7. di Luccio E, Azulay D, Regaya I, Fajloun Z, Sandoz G, Mansuelle P, Kharrat R, Fathallah M, Carrega L, Estéve, E, and others. Parameters affecting in vitro oxidation/folding of maurotoxin, a four-disulphide-bridged scorpion toxin. Biochem J 2001;358:681-692.
8. di Luccio E, Matavel A, Opi S, Regaya I, Sandoz G, M'Barek S, Carlier E, Estéve E, Carrega L, Fajloun Z, and others. Evolution of maurotoxin conformation and blocking efficacy towards Shaker B channels during the course of folding and oxidation in vitro. Biochem J 2002;361:409-416.
9. Regaya I, Beeton C, Ferrat G, Andreotti N, Darbon H, De Waard M, Sabatier JM. Evidence for domain-specific recognition of SK and Kv channels by MTX and HsTx1 scorpion toxins. J Biol Chem 2004;279:55690-55696.
10. Holaday SK, Martin BM, Fletcher PR, Krishna NR. NMR solution structure of butantoxin. Arch Biochem Biophys 2000;379:18-27.
11. Pimenta AM, Mansuelle P, Diniz CR, Martin-Eauclaire MF. Covalent structure and some pharmacological features of native and cleaved alpha-KTx12-1, a four disulfide-bridged toxin from Tityus serrulatus venom. J Peptide Sci 2003;9:132-140.
12. + channels, types Kv1.1, 1.2, 1.3, 1.5, and 3.1, stably expressed in mammalian cell lines. Mol Pharmacol 1994;45:1227-1234.
13. Merrifield RB. Solid phase synthesis. Science 1986;232:341-347.
14. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ. Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Arch 1981;391:85-100.
15. Rauer H, Grissmer S. Evidence for an internal phenylalkylamine action on the voltage-gated potassium channel Kv1.3. Mol Pharmacol 1996;50:1625-1634.
16. Marion D, Ikura M, Tschudin R, Bax A. Rapid recording of 2D NMR spectra without phase cycling. Application to the study of hydrogen exchange in proteins. J Magn Res 1989;85:393-399.
17. Marion D, Wüthrich, K. Application of phase sensitive two-dimensional correlated spectroscopy (COSY) for measurements of 1H-1H spin-spin coupling constants in proteins. Biochem Biophys Res Commun 1983;113:967-974.
18. Piotto M, Saudek V, Sklenar V. Gradient-tailored excitation for single-quantum NMR spectroscopy of aqueous solutions. J Biomol NMR 1992;2:661-665.
19. Wüthrich, K, editor. NMR of proteins and nucleic acids. New-York: Wiley; 1986.
20. Eccles C, Guntert P, Billeter M, Wüthrich, K. Efficient analysis of protein 2D NMR spectra using the software package EASY. J Biomol NMR 1991;1:111-130.
21. Linge JP, Habeck M, Rieping W, Nilges M. ARIA: automated NOE assignment and NMR structure calculation. Bioinformatics 2003; 19:315-316.
22. Brunger AT, Adams PD, Clore GM, DeLano WL, Gros P, Grosse-Kunstleve RW, Jiang JS, Kuszewski J, Nilges M, Pannu NS, and others. Crystallography & NMR system: a new software suite for macromolecular structure determination. Acta Crystallogr D Biol Crystallogr 1998;54:905-921.
23. Roussel A, Cambillau C. Silicon Graphics Geometry Partner Directory. Mountain View, CA: Silicon Graphics; 1989. p 77-78.
24. Laskowski RA, Rullmannn JA, MacArthur MW, Kaptein R, Thornton JM. AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 1996;8:477-486.
25. Jiang Y, Lee A, Chen J, Ruta V, Cadene M, Chait BT, Mackinnon R. X-ray structure of a voltage-dependent K+ channel. Nature 2003;423:33-41.
26. Palma PN, Krippahl L, Wampler JE, Moura JJ. BiGGER: a new (soft) docking algorithm for predicting protein interactions. Proteins 2000;39:372-384.
27. Mouhat S, Jouirou B, Mosbah A, De Waard M, Sabatier JM. Diversity of folds in animal toxins acting on ion channels. Biochem J 2004;378:717-726.
28. Nicholls A, Sharp KA, Honig B. Protein folding and association: insights from the interfacial and thermodynamic properties of hydrocarbons. Proteins 1991;11:281-296.
29. Stocker U, van Gunsteren WF. Molecular dynamics simulation of hen egg white lysozyme: a test of the GROMOS96 force field against nuclear magnetic resonance data. Proteins 2000;40:145-153.
30. Wallace AC, Laskowski RA, Thornton JM. LIGPLOT: a program to generate schematic diagrams of protein-ligand interactions. Protein Eng 1995;8:127-134.
31. Carlier E, Fajloun Z, Mansuelle P, Fathallah M, Mosbah A, Oughideni R, Sandoz G, di Luccio E, Geib S, Regaya I, and others. Disulfide bridge reorganization induced by proline mutations in maurotoxin. FEBS Lett 2001;489:202-207.
32. Fajloun Z, Ferrat G, Carlier E, M'Barek S, Regaya I, Fathallah M, Rochat H, Darbon H, De Waard M, Sabatier JM. Synthesis, 3-D structure, and pharmacology of a reticulated chimeric peptide derived from maurotoxin and Tsk scorpion toxins. Biochem Biophys Res Comm 2002;291:640-648.
33. +-channels. Toxicon 2004;43:865-875.
34. Bontems F, Roumestand C, Gilquin B, Ménez A, Toma F. Refined structure of charybdotoxin: common motifs in scorpion toxins and insect defensins. Science 1991;254:1521-1523.
35. Blanc E, Sabatier JM, Kharrat R, Meunier S, El Ayeb M, Van Reitschoten J, Darbon H. Solution structure of maurotoxin, a scorpion toxin from Scorpio maurus, with high affinity for voltage-gated potassium channels. Proteins 1997;29:321-333.
36. Johnson WC. Circular dichroism and its empirical application to biopolymers. Methods Biochem Anal 1985;31:61-163.
37. + channels. Mol Pharmacol 1996;50:1167-1177.
38. Fu W, Cui M, Briggs JM, Huang X, Xiong B, Zhang Y, Luo X, Shen J, Ji R, Jiang H, and others. Brownian dynamics simulations of the recognition of the scorpion toxin maurotoxin with the voltage-gated potassium ion channels. Biophys J 2002;83:2370-2385.